The invention relates to a rhubarb health care toothpaste and is applied to teeth cleaning and health care of users in daily life. The rhubarb toothpaste comprises, by weight, 40-50kg of calcium carbonate, 15-25kg of sorbitol, 0.2-1.5kg of sodium carboxy methyl cellulose, 0.1-1kg of guar gum, 0-0.5kg of saccharin sodium, 0.5-2.5kg of sodium lauryl sulfate, 0-1kg of sodium pyrophosphate, 1-5kg of silicon dioxide, 0-0.5kg of sodium methyl, 1-3.5kg of propylene glycol, 15-30kg of deionized water, 0-0.8kg of essence, 0-1kg of menthol crystal, 0.2-1.5kg of rhubarb biological extract, and 0-0.8kg of sea-buckthorn extract. A preparation method of the rhubarb toothpaste includes adding and evenly mixing the sorbitol, the sodium carboxy methyl cellulose, the guar gum, the sodium lauryl sulfate, the sodium pyrophosphate, the silicon dioxide, the sodium methylparaben and the propylene glycol in a vacuum unguent machine; adding deionized water; adding sequentially the rhubarb biological extract, the sea-buckthorn extract, the saccharin sodium, the calcium carbonate, the menthol crystal and the essence; and performing stirring in high speed, fully emulsification and even mixing in a vacuum state.