Sea lettuce polysaccharide, preparation and use thereof

Abstract

The invention relates to Monostroma nitidum polysaccharide, which is characterized in that: sugar chains of the Monostroma nitidum polysaccharide contain rhamnose groups, xylose groups, glucose groups, galactose groups, mannose groups and glucuronic acid groups, and the rhamnose group polysaccharide is a linear long chain molecule which is connected by alpha-1, 2-glycosidic bonds and alpha-1, 3-glycosidic bonds. Under the condition of preparation, algae frond is immersed into water for homogenate, and then heated and extracted, and a clear solution is separated, concentrated and desalinated; the desalinated solution is concentrated and deproteinized; the deproteinized solution is concentrated, alcohol deposited, dried, dissolved and then separated through an ion exchange chromatographic column and a gel chromatographic column in turn; the collected solution is concentrated and desalinated; and the desalinated solution is concentrated, alcohol deposited, cleaned, dried and crushed. The Monostroma nitidum polysaccharide can be used for preparing a novel anticoagulant or antithrombotic reagent.

Description

technical field [0001] The invention relates to a reef membrane polysaccharide, a preparation method and application thereof. Background technique [0002] Studies have found that heparin, which has been widely used as an anticoagulant drug for more than 70 years, has many side effects, such as inducing thrombocytopenia and bleeding. In addition, the concentration of heparin in the main raw material for preparing heparin is low, and the occurrence of "mad cow disease" related to cattle makes it urgent to find alternatives to anticoagulant drugs. In recent years, in addition to continuing to research and develop low-molecular-weight heparins, such as enoxaparin, nadroparin calcium, dalteparin sodium and tinzaparin sodium, people have also modified the structure of some natural polymers according to the structural characteristics of heparin to achieve similar purpose. Experiments have proved that these heparins have certain anticoagulant activity, but they also have certain ...

AI Technical Summary

Problems solved by technology

At present, the annual global market value of antithrombotic drugs is about 12 billion U.S. dollars, and is increasing at an annual rate of 15%; the global market value of anticoagulant drugs is about 4 billion U.S. dollars, and is increasing at a rate of 13%. Coagulation and antithrombotic drugs are far from meeting the growing needs of people

Seaweed is a natural and rich source of anticoagulant polysaccharides. At present, the research and development of seaweed polysaccharides mainly focus on red algae polysaccharides and fucoidan, while the research and development of green algae polysaccharides is rare, and the literature on reef polysaccharides has not been reported yet.

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