1498 results about "Rhamnose" patented technology

The present invention provides an isolated microorganism strain, Lactobacillus rhamnosus GM-020, which is found to be effective in treating obesity and a complication thereof. The use of the Lactobacillus rhamnosus GM-020 in treating obesity and a complication thereof is also provided.

The invention provides a method for preparing germinated brown rice milk beverage by compound lactobacillus fermentation, which comprises the following steps of: preparing four lactobacillus direct-throwing fermentation agents such as lactobacillus rhamnosus, pediococcus acidilactici, streptococcus thermophilus and lactobacillus brevis; then soaking brown rice for 10 hours at 30 DEG C, and germinating for 12 hours at 2 DEG C to prepare germinated brown rice; adding 3 times water into the germinated brown rice, grinding milk by adopting colloid, gelatinizing and saccharifying the milk, adding 150 grams of direct-throwing fermentation agents into each ton of liquid rice milk, and performing standing fermentation for 6 hours at the temperature of between 38 and 40 DEG C; and centrifuging the liquid rice milk at 5,000 revolutions per minute, removing residue, adding xylitol according to the clear liquid volume for blending, filtering the liquid by using a hollow fiber membrane, performing ultrahigh-temperature instantaneous sterilization for 2 seconds at 143 DEG C, and filling the liquid with pillow-type package or tetra package to obtain a finished product.

The invention discloses a preparing method of sweet chrysanthemum glycosides and chromocor extract from sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, labroid glycosides A, cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside], derivant and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; producing extract; counting 5-100%(w / w) of each sweet chrysanthemum glycosides element and chromocor element content in sweet chrysanthemum glycosides and chromocor extract; setting the chromocor element sum in sweet chrysanthemum glycosides, labroid glycosides A, cyanidenon-7-0-beta-D glycosides, quercetin, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] at 5-100%(w / w).

The present disclosure generally relates to pediatric nutritional compositions including a prebiotic mixture of galacto-oligosaccharide and / or polydextrose, a probiotic, such as Lactobacillus rhamnosus GG, and human milk oligosaccharides. More particularly, the present disclosure relates to a nutritional composition having: (i) a protein source, (ii) a lipid source, (iii) a carbohydrate source, (iv) a human milk oligosaccharide or a precursor thereof, (v) polydextrose and / or galacto-oligosaccharides, and (vi) a probiotic. The disclosed nutritional compositions advantageously promote the gut-brain axis.

The invention discloses a preparation method for fast separating flavonoid glycosides from oil-tea-cakes with a medium pressure column, which comprises the following steps of: decorticating and crushing camellia oleifera abel seeds, degreasing the camellia oleifera abel seeds with non-polar solvents, performing extraction with ethanol water, filtering and condensing the extract to obtain crude extract concrete, performing fast separation with the medium pressure column to obtain an over 90 percent flavonoid glycoside mixture, and further adopting a high performance liquid chromatography to prepare over 95 percent flavonoid glycoside monomers, wherein the flavonoid glycoside monomers are kaemplerol 3-O-[2-O-beta-D-galactose-6-O-alpha-L-rhamnose]-beta-D-glucoside (I) and kaemplerol 3-O-[2-O-beta-D-xylose-6-O-alpha-L-rhamnose]-beta-D-glucoside (II) respectively. The method can be used for batch preparation, and has the advantage of providing quality raw materials for the development of flavonoid glycoside medicaments and healthcare functional products in the oil-tea-cakes.

A two-column process for extracting L-arabinose from acacia gum includes hydrolyzing in inorganic acid, alkali neutralizing, extracting in alcohol, filtering, dissolving in acetic acid at 60-90 deg.Cto obtain coarse L-arabinose mixture, separating by the first column to obtain the mixture of pure rhamnose and mixture of L-arabinose and galactose, and separating by the second column to obtain pure L-arabinose. The cellulose, alumina, starch, silica gel and developing solvent are filled in columns. Its advantages are low cost and high yield and purity of product.

The invention relates to a composite soil conditioner, comprising biogas residues, pyroligneous acid, mineral powder, soluble chitosan, diethylene triaminepentaacetic acid pentasodium, a surfactant, tobacco powder, trehalose, di-rhamnolipid, a flocculating component, a penetrating agent, enteromorpha powder, modified zeolite, boron-zinc-magnesium-containing fungicide, a nano additive, an accelerant, pentaerythritol triacrylate and ionic liquid, preferably also comprising 4-methyl phenyl valerate which is equal to the ionic liquid in weight. The novel composite soil conditioner has excellent soil improvement and recovery performance, the utilization rate of the fertilizer by the crop is greatly increased, and the composite soil conditioner can significantly increase the yield, simultaneously also can improve the permeability of the soil and avoids side effects of heavy metals such as cadmium; thus, the composite soil conditioner is beneficial to crop growth and has a wide industrial application prospect.

The invention relates to a bacterium S2 for efficiently generating biosurfactant and a fermentation culture medium thereof, belonging to the field of biotechnology. The bacterium S2 is gram-negative bacterium which is rod-shaped, is (6-8)*(8-12) micrometers in size and has no spores and capsules. The bacterial strain belongs to the pseudomonas aeruginosa through identifying by morphological and physio-biochemical characteristics and molecular biology, and the collection code is CGMCC No.3034. The best fermentation culture medium is a novel culture medium which takes 50g / L of rapeseed oil as the only carbon source. The rhamnolipid is the only fermentation product of the bacterial strain and has high yield. The CMC (Critical Micelle Concentration) value of the fermentation liquid is 0.25 g / L; compared with the common chemical surfactant, the bacterium has more obvious emulsification and durability on hydrophobic substances, such as oils and the like.

The invention relates to a method for preparing icaritin through converting total flavones of epimedium by an enzyme method. The method comprises the step of converting multicomponent flavone glycosides in the total flavones of epimedium by a dual-enzyme system consisting of heat-resistant alpha-L-rhamnosidase and heat-resistant beta-glucosidase, so as to produce the icaritin. According to the method, multicomponent flavone compounds (such as icariin, Epimedin A, Epimedin B and Epimedin C) in the total flavones of epimedium can be almost completely converted into the target product; the optimal acting temperature is high, so that a substrate and intermediate products have good solubility, and a cosolvent is not required; and the enzymolysis time is short, and the yield is high. The icaritin prepared by the method has a remarkable higher propagation inhibiting effect on liver cancers, lung cancers, colon cancers and mammary cancers compared with each monomer of the total flavones of epimedium before conversion.

The invention relates to application of an alpha-L-rhamnoside enzyme in the directional synthesis of isoquercitrin by the biological conversion of rutin. Because the crude enzyme preparation or the immobilized enzyme preparation of the alpha-L-rhamnoside enzyme is used for catalyzing and hydrolyzing the rutin and directionally and biologically synthesizing the isoquercitrin, the application has the advantages of wide source, easy preparation and low cost of catalysts, high stability, and high catalysis efficiency and specificity of the enzyme preparation, and is easy to store. Thus, the alpha-L-rhamnoside enzyme can greatly reduce the production cost of quercetin, the catalysis and conversion rate of the enzymes is high, and the products are single.

The invention discloses a rhamnolipid high-yielding bacterial strain, and applications thereof. The rhamnolipid high-yielding bacterial strain is named as pseudomonas aeruginosa KT1115, is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC M 2016686. The rhamnolipid high-yielding bacterial strain is stable in hereditary features and high in rhamnolipid yield, and is obtained via ARTP induced mutation, blue gel plate screening, and secondary screening. According to fermentation broth of pseudomonas aeruginosa KT1115, liquid surface tension is reduced to 24.8mN / m<2>, and emulsification value is as high as 80%. Optimization of fermentation conditions including carbon source and nitrogen source single factors, cheap crude glycerine and swill-cooked dirty oil are taken as a composite carbon source, so that production cost is reduced greatly. The rhamnolipid yield of pseudomonas aeruginosa KT1115 is 50.7g / L, is increased by 18.1 times compared with that of a starting strain, conversion rate is 0.75g / g, and is relatively high. Application prospect of pseudomonas aeruginosa KT1115 in further industrialized application is promising.

A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xylanase) and a β-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic, endogenous microflora-promoting strain; or combinations thereof. The DFM may be selected from the group consisting of: Bacillus subtilis AGTP BS3BP5, Bacillus subtilis AGTP BS442, B. subtilis AGTP BS521, B. subtilis AGTP BS918, Bacillus subtilis AGTP BS1013, B. subtilis AGTP BS1069, B. subtilis AGTP 944, B. pumilus AGTP BS 1068 or B. pumilus KX11-1, Enterococcus faecium ID7, Propionibacterium acidipropionici P169, Lactobacillus rhamnosus CNCM-1-3698, Lactobacillus farciminis CNCM-1-3699, a strain having all the characteristics thereof, any derivative or variant thereof, and combinations thereof and the further fibre degrading enzyme may be selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91), a β-glucosidase (E.C. 3.2.1.21), a β-xylosidase (E.C. 3.2.1.37), a feruloyl esterase (E.C. 3.1.1.73), an α-arabinofuranosidase (E.C. 3.2.1.55), a pectinase (e.g. an endopolygalacturonase (E.C. 3.2.1.15), an exopolygalacturonase (E.C. 3.2.1.67) or a pectate lyase (E.C. 4.2.2.2)), or combinations thereof.

The invention relates to a microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as a preparation method and an application of the microbial agent. The microbial agent comprises the following effective components: mycobacterium gilvum CP13, trichoderma viride and rhamnolipid. The preparation method comprises the following steps: mixing bacterial solutions obtained by respectively culturing mycobacterium gilvum CP13 and trichoderma viride, simultaneously adding a composite carrier of diatomaceous earth and modified peanut shells and rhamnolipid and carrying out microbial immobilization to prepare the microbial agent. The microbial agent has super strong polycyclic aromatic hydrocarbons removal capability and meanwhile has the advantages of low cost and long shelf life; and by virtue of the microbial agent, over 90% of polycyclic aromatic hydrocarbons, such as high-concentration (50mg / kg) pyrene contained in the vegetable garden soil can be removed under conditions of room temperature (20 + / -5 DEG C) and pH of 4-9 within 36 days.

The present invention discloses lactobacillus rhamnosus, named lactobacillus rhamnosus HCS01-003. The strain is preserved on August 14, 2018 in the "China General Microbiological Culture Collection Center", and the preservation number is CGMCC No.16257. The invention also discloses an application of the strain used as an additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing foods, health foods or pharmaceutical compositions. The invention further discloses an application of the strain used as a food additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing infant foods.

The invention discloses an enteromorpha se-polysaccharide with a biological activity, which is characterized in that the enteromorpha se-polysaccharide is heteropolysaccharide consisting of rhamnose, xylose, mannose, glucose and selenide groups. The invention further discloses a preparation method and an application of enteromorpha se-polysaccharide. According to the invention, an enteromorpha se-polysaccharide pure product with high added values and biological activity is prepared, selenium-containing content of polysaccharide is improved, so that physiological and pharmacological functions of selenium and enteromorpha se-polysaccharide are optimized, the activity is higher than selenium and enteromorpha se-polysaccharide, enteromorpha se-polysaccharide is beneficial to absorption and utilization of a human body, waste is changed into things of value, and enteromorpha se-polysaccharide has good social benefits, economic benefits and a wide application prospect.

The invention provides a preparation method of probiotic fermented rice milk. Three probiotics of lactobacillus rhamnosus, lactobacillus paracasei subsp. paracasei, and lactobacillus brevis are used as original strains, and compound probiotic freeze-dried powder is obtained through amplification culture, powdery freeze-dried strain preparation, and freeze-dried powder complex formulation. The probiotic fermented rice milk is prepared by the following steps: obtaining rice, brown rice and drinking water according to a weight ratio of 2:1:7, performing pulp refining in a colloid mill, sieving by a 80-mesh fine sieve to prepare mixed rice milk pulp, adding 5% white sugar, performing liquefaction by high temperature resistant alpha-amylase, performing saccharification by glucoamylase, performing hydrolyzation by papain, inoculating the compound probiotic freeze-dried powder according to a ratio of 0.01%, allowing the powder to stand and performing fermentation for 4 hours, well mixing, and performing aseptic filling.

The invention discloses a method for preparing neohesperidin by an enzymic method, comprising the following steps: performing coordination between hesperidin and metal ions and dissolving in water, and realizing conversion from hesperidin to neohesperidin under the action of immobilized rhamnosidase and immobilized rhamnoside transferase. The method provided by the invention is adopted to solve the problem that production cost of neohesperidin widely used in medical, functional food and its cosmetic fields is high, and simultaneously opens up a new approach for recycling of industrial waste orange peel and high value-added utilization of hesperidin.

The invention relates to a process of recovering arabinose and optionally other monosaccharides from vegetable fiber rich in heteropolymeric arabinose, such as gum arabic. Said other monosaccharides are typically selected from galactose and rhamnose. The process of the invention comprises controlled hydrolysis of the arabinose-rich vegetable fiber and fractionation of the hydrolysis product to obtain a fraction enriched in arabinose and optionally other product fractions followed by crystallization of arabinose. The invention also relates to a novel method of crystallizing arabinose from biomass-derived material. Furthermore, the invention relates to novel crystalline L-arabinose.

The invention discloses an effective part of stevia and the activity and the application thereof. The effective part mainly comprises stevioside category and flavone category which are obtained by extracting and separating from dried stevia leaves, wherein the sum of the percentage content of the stevioside components in the stevioside part is 5 to 100 percent (w / w), and the stevioside components mainly contains the stevioside, stevibioside, rebaudioside A, B, C, D, E, and F, dulcoside A and the derivative thereof, etc.; the sum of the percentage content of the flavone components in the flavone part is 5 to 100 percent (w / w), and the flavone components mainly contains luteolin, quercetin, luteolin-7-O-Beta-D-glucoside, apigenin-7-O-Beta-D-glucoside, quercitrin, quercetin-3-O-Beta-D-arabinoside, quercetin-3-O-(4-O-anti form-caffeoyl acyl-Alpha-L-rhamnose-(1 to 6)-Beta-D-galactoside) and the derivative thereof, etc., and 4, 5-dicaffeoylquinic acid and the derivative thereof, etc. The effective part has significant sugar-reducing and fat-reducing effects, can be used singly or combined with any other Chinese medicines and Western medicines or foods in any proportion, is used for preparing medicines or functional foods, and is used for treating hyperglycemia and hyperlipoidemia.

Alpha-L-rhamnosidase and a preparing method and applications thereof are disclosed. The amino acid sequence of the alpha-L-rhamnosidase is shown as SEQ ID NO.1. The recombinase is high in optimum temperature, good in thermal stability, and capable of efficiently expressing target protein under preferred conditions, so that the recombinase has wide applications in the fields of selective hydrolysis of rutoside, hesperidin, naringin, prunin, saikoside and other flavonoid compounds connecting alpha-rhamnoside or rhamnose in terpene fragrance precursor compounds, and the like.

The invention discloses a method for separating and purifying a flavone compound of momordica grosvenori leaves by HSCCC and the product thereof, wherein, the method of the invention comprises the following steps: the grosvenori leaves are added with water or other polar solvents so as to carry out reflux extraction; the obtained extracts adopt the mixed solution of ethyl acetate, normal butanol and water as a solvent system and are separated and purified by a preparation-type high-speed counter current chromatograph, and then kaempferol-3-O-Alpha-L- rhamnose-7-O-[Beta-D-glucosyl group(1-2)-O-L-rhamnoside] and kaempferol-3,7-Alpha-L-2- rhamnoside. In the method, by applying the HSCCC method to separate and purify the extracts of the momordica grosvenori leaves, two flavone compounds with higher purity are successfully obtained by separation, thereby extending the source of the raw materials for flavone products; moreover, the method has simple preparation technique, easy operation and high purification efficiency, lays the solid first stone for extraction and utilization of the effective components in the momordica grosvenori leaves, and can avoid environment pollution caused by the waste and throw of a large amount of momordica grosvenori leaves.

The invention belongs to the field of health-care products, and particularly relates to a probiotics and Chinese herbal medicine compound preparation with a hypoglycemic function. The preparation is formed by mixing the components in parts by weight as follows: 25-30 parts of dioscorea roots, 15-25 parts of pueraria roots, 20-30 parts of cinnamon, 20-40 parts of lycium berries and 30-40 parts of freeze-dried probiotics powder, wherein probiotics comprises bacteria in percentage by weight as follows: 40% of bifidobacterium adolescentis, 40% of bifidobacterium longum, 10% of lactobacillus plantarum and 10% of lactobacillus rhamnosus. The preparation has the hypoglycemic function.

The invention relates to preparation of polycarboxylate superplasticizer, in particular to polycarboxylate superplasticizer with improved workability and encapsulation and a preparation method thereof. An effective dose of monomers, polysaccharide with the molecule side chain containing rhamnose, hydroxide radical and carboxyl, sodium hypophosphite, sodium bisulfoxylate formaldehyde powder, vitamin C, sodium hydrogen sulfite, crylic acid, hydrogen peroxide and sodium hydroxide are dissolved in water and then subjected to blending, fed-batch blending and other steps, and then the polycarboxylate superplasticizer is obtained. The technical problems that in the prior art, during using, the process operation is complex are solved. The polycarboxylate superplasticizer has the advantages that the preparation process and a use method are simple, the polycarboxylate superplasticizer meets national standard requirements, and after being used, the polycarboxylate superplasticizer is good in workability and does not separate or bleed.

The invention provides a probiotic composition and a preparation method and applications thereof, and belongs to the technical field of health-care foods. The probiotic composition comprises probiotics and prebiotics. The probiotics comprise lactobacillus acidophilus NCFM, lactobacillus rhamnosus HN001, lactic acid bacteria composite powder GI, lactic acid bacteria composite powder OH, bifidobacterium animalis B94, bifidobacterium bifidum R0071, lactobacillus casei R0215, live bacillus coagulans GBI30,6086 and lactobacillus rhamnosus GG. The prebiotics include fructo-oligosaccharides, resistant dextrins and lactitol. The mass ratio of the probiotics to the prebiotics is (300-350):(1300-1400). The total viable bacteria concentration of the probiotics in the probiotics composition is more than or equal to 9.0x10<9> CFU / g. The probiotic composition provided by the invention can obviously improve sleep quality, and significantly relieve anxiety and depression.

The invention discloses two types of flavonoid glycoside compounds which are kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(2'' ', 4'' '-O-diacetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-glucopyrose galactopyranoside and kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(4'' '-O-acetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-D-glucopyrose galactopyranoside respectively. The invention also discloses a method for preparing the two types of flavonoid glycoside compounds. Moreover, pharmacological tests prove that the two types of flavonoid glycoside compounds have quite good effects of reducing blood sugar and blood fat and resisting oxidation and cerebral ischemia, and thus, the flavonoid glycoside compounds of the invention can be used for preparing medicaments, food and cosmetics for preventing and treating diabetes, reducing blood fat and resisting oxidation and cerebral ischemia.

The invention relates to lactobacillus rhamnosus grx19 and its application. The collection number of the lactobacillus rhamnosus grx19 is CGMCC No.5519. The stain can be used to produce live bacteria lactobacillus beverages with long shelf-life and fermented dairy products. Lactobacillus rhamnosus grx19 is taken as culture starter alone or in combination with other lactic acid bacterias and then live bacteria lactobacillus beverages and fermented dairy products are prepared according to routine methods. After heat treatment at the temperature of 70-75 DEG C for 15-20 seconds, by the adoption of aseptic filling or sanitary filling, live bacteria lactobacillus beverages and fermented dairy products sold at room temperature can be produced.

The invention discloses aprobiotic solid beverage for infants. The probioticsolid beverage comprises components in percentage by weight as follows: 15-25% of bifidobacterium powder, 15-25% of lactobacillus rhamnosus powder, 10-30% of rice protein powder, 10-20% of bovine colostrum powder, 5-15% of maltodextrin, 5-10% of fructo-oligosaccharide and 5-10% of galactooligosaccharide. The probioticsolid beverage is natural in component, scientific in formula, balanced in nutrition, convenient to drink, free of caking, good in dissolving ability and unique in taste and has effects of improving intestinal tracts of the infants, improving digestion and enhancing the immunity.