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Method for separating and purifying Momordica grosvenori leaf chromocor compound by high-speed countercurrent chromatography and products thereof

A high-speed counter-current chromatography, separation and purification technology, which is applied in the field of high-speed counter-current chromatography for separation and purification of flavonoids in Luo Han Guo leaves, can solve the problems of complex structures of flavonoids, and achieve the effects of broadening the source of raw materials, simple preparation process and easy operation.

Inactive Publication Date: 2009-01-28
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are some flavonoids with complex structures, including alkaloid flavones such as ficus and isoficine.
However, there is no report on the separation and purification of a large number of flavonoid monomers with higher purity from the leaves of Luo Han Guo

Method used

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  • Method for separating and purifying Momordica grosvenori leaf chromocor compound by high-speed countercurrent chromatography and products thereof
  • Method for separating and purifying Momordica grosvenori leaf chromocor compound by high-speed countercurrent chromatography and products thereof
  • Method for separating and purifying Momordica grosvenori leaf chromocor compound by high-speed countercurrent chromatography and products thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 of the present invention: the steps of separating and purifying flavonoids from Luo Han Guo leaves by high-speed countercurrent chromatography are as follows:

[0019] (1) Extraction of Luo Han Guo leaves: Weigh non-rotten dried Luo Han Guo leaves, add water (the water must completely submerge the Luo Han Guo leaves), boil and extract 3 times, each time for 50 minutes, filter, combine the filtrates and spin dry to a paste, and obtain the rough extraction of Luo Han Guo leaves things, spare.

[0020] (2) Solvent preparation: put ethyl acetate, n-butanol and water with a volume ratio of 4:1:5 in a separatory funnel, vibrate vigorously to balance the two phases, separate after standing and layering, The lower phase was the mobile phase, and the upper phase was the stationary phase, which were subjected to ultrasonic degassing for 30 minutes, and cooled naturally for later use.

[0021] (3) Sample preparation: accurately weigh 90 mg of Luo Han Guo leaf crude e...

Embodiment 2

[0023] Embodiment 2 of the present invention: the steps of separating and purifying flavonoids from Luo Han Guo leaves by high-speed countercurrent chromatography are as follows:

[0024] (1) Extraction of Luo Han Guo leaves: Weigh Luo Han Guo leaves, add 60% ethanol, reflux extraction at 80°C for 3 times, 60 min each time, filter, combine the filtrates and concentrate to a paste to obtain a crude Luo Han Guo leaf extract, which is set aside.

[0025] (2) Preparation of solvent: mix ethyl acetate, n-butanol and water with a volume ratio of 3.6: 1.2: 5.2, oscillate and shake evenly, so that the two phases reach equilibrium, separate after standing and layering, and the lower phase is the mobile phase. The upper phase was the stationary phase, which were subjected to ultrasonic degassing for 40 minutes, and cooled for later use.

[0026] (3) Sample preparation: accurately weigh 90 mg of Luo Han Guo leaf crude extract, dissolve in equal volumes of upper phase and lower phase solv...

experiment example

[0029] 1.1 Materials and Instruments

[0030] Material: Mangosteen leaves (provided by Guilin Yiyuansheng Modern Biotechnology Co., Ltd.)

[0031] Instruments: TBE-300 high-speed countercurrent chromatograph (Shanghai Tongtian Biochemical Technology Co., Ltd.); TBP-50A constant pressure and constant flow micropump (Shanghai Tongtian Biochemical Technology Co., Ltd.); 8823B ultraviolet detector (Beijing Binda Ying Chuang Technology Co., Ltd., Beijing New Technology Application Research Institute); HX-1050 constant temperature circulator (Beijing Boyikang Experimental Instrument Co., Ltd.); SHB-IIIA circulating water multi-purpose vacuum pump (Zhengzhou Great Wall Science and Trade Co., Ltd.); AR1140 / C-type electronic analytical balance; Shimadzu high-performance liquid chromatography (Shimadzu International Trading (Shanghai) Co., Ltd.); UV-1100 ultraviolet-visible spectrophotometer; RE-52AA rotary evaporator; DZF-150 digital display small vacuum drying Box; SHB-III circulati...

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Abstract

The invention discloses a method for separating and purifying a flavone compound of momordica grosvenori leaves by HSCCC and the product thereof, wherein, the method of the invention comprises the following steps: the grosvenori leaves are added with water or other polar solvents so as to carry out reflux extraction; the obtained extracts adopt the mixed solution of ethyl acetate, normal butanol and water as a solvent system and are separated and purified by a preparation-type high-speed counter current chromatograph, and then kaempferol-3-O-Alpha-L- rhamnose-7-O-[Beta-D-glucosyl group(1-2)-O-L-rhamnoside] and kaempferol-3,7-Alpha-L-2- rhamnoside. In the method, by applying the HSCCC method to separate and purify the extracts of the momordica grosvenori leaves, two flavone compounds with higher purity are successfully obtained by separation, thereby extending the source of the raw materials for flavone products; moreover, the method has simple preparation technique, easy operation and high purification efficiency, lays the solid first stone for extraction and utilization of the effective components in the momordica grosvenori leaves, and can avoid environment pollution caused by the waste and throw of a large amount of momordica grosvenori leaves.

Description

(1) Technical field: [0001] The invention relates to a method for separating and purifying flavonoid compounds from Luo Han Guo leaves by high-speed countercurrent chromatography and a product thereof, and belongs to the technical field of purification of active ingredients used in traditional Chinese medicine from Luo Han Guo leaves. (two) background technology: [0002] As a traditional specialty in northern Guangxi, Luo Han Guo is also a unique medicinal plant in my country. It has the reputation of "Oriental God Fruit" and has a long-standing reputation in China, Hong Kong, Macao, Southeast Asia, and European and American countries. It is mainly distributed in Guangdong, Guangxi, Yunnan, Jiangxi, and southern Hunan. It is mainly produced in Yongfu, Lingui, and Longsheng counties in Guangxi. There are 7 species of Luo Han Guo plants, among which Luo Han Guo (Siraitia grosvenorii Swingle) and winged Luo Han Guo [S.siamensis (Craib) C. Jeffrey] can be used as medicine, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K36/42
Inventor 潘英明朱志仁王恒山
Owner GUANGXI NORMAL UNIV
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