Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

619 results about "Countercurrent chromatography" patented technology

Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by centrifugal force and is used to separate, identify, and quantify the chemical components of a mixture. In its broadest sense, countercurrent chromatography encompasses a collection of related liquid chromatography techniques that employ two immiscible liquid phases without a solid support. The two liquid phases come in contact with each other as at least one phase is pumped through a column, a hollow tube or a series of chambers connected with channels, which contains both phases. The resulting dynamic mixing and settling action allows the components to be separated by their respective solubilities in the two phases. A wide variety of two-phase solvent systems consisting of at least two immiscible liquids may be employed to provide the proper selectivity for the desired separation.

Novel valve and process for interrupted continuous flow chromatography

A unique valve assembly for simulated bed moving chromatography employs a combination of pressure compatible sealable materials that permit minimal frictional interaction between moving parts, ensures alignment of parts, and provides a method for conducting SMB countercurrent chromatography on a small scale, in which purity and separation efficiency are enhanced. Machine designs are disclosed which embody the SMB concepts. Finally, a quick disconnect connector and adaptor is provided for mounting chromatographic columns in an SMB device. Demonstration of this system in a fructose-glucose isomer separation with purity-recovery trade-off for this device is also presented.
Owner:SEMBA

Method for separating purified pelargonidin anthocyanin monomer from rubus hirsutus

InactiveCN106831911AOvercome the disadvantages such as adsorption lossLarge injection volumeSugar derivativesSugar derivatives preparationCountercurrent chromatographyAlcohol
The invention discloses a method for separating a purified pelargonidin anthocyanin monomer from rubus hirsutus. According to the method, high-purity pelargonidin-3-O-glucoside is obtained by separated and purificated from the rubus hirsutus through alcohol extraction concentration, ethyl acetate extraction, AB-8 macroporous resin adsorption and high-speed countercurrent chromatography, and the purity of the pelargonidin-3-O-glucoside is greater than 99 percent. By using the method, 113.3mg of pelargonidin-3-O-glucoside can be obtained from 1000g of fresh rubus hirsutus fruits; compared with a traditional separation method, the purity and yield thereof are improved; moreover, the process is simple, so industrialized production can be realized, and a new idea is provided for comprehensive development and utilization of rubus hirsutus resources of China.
Owner:ZHEJIANG UNIV +1

Extraction method and and purification method of Sanghuang mushroom polysaccharide

The invention pertains to the field of extracting active ingredients of natural products and particularly relates to an extracting method and a purifying method of crude phellinus igniarius polysaccharides. The technical problems needed to be solved are to disclose a method for extracting the extract of crude phellinus igniarius polysaccharides and an efficient method for preparing phellinus igniarius polysaccharides through countercurrent chromatography. An ultrasonic extraction is adopted by the invention to extract crude phellinus igniarius polysaccharides from phellinus igniarius fruit bodies; the high-speed countercurrent chromatography is adopted to further purify the crude phellinus igniarius polysaccharides. The extracting method and the purifying method of the invention have high yield of crude phellinus igniarius polysaccharides, and can take up 6 percent of crude drugs; the purity of the further purified phellinus igniarius polysaccharides is greatly improved to be about 75 percent.
Owner:SHANGHAI PUCHENG BIOTECHOLOGY

New method for extracting and separating high-purity huperzine A from thousand-layer column

The invention relates to a new method for extracting and separating high-purity huperzine A from a thousand-layer column. The method comprises the following three technological steps: first, the preparation of extract liquor, second, the pre-separation of macroporous resin, and third, the purification by high-speed countercurrent chromatogram. The preparation of the extract liquor comprises immersing the grinded thousand-layer column in acid liquor, implementing diacolation and ultrasonic extraction, and obtaining the extract liquor for further use. The pre-separation of the macroporous resin comprises regulating the pH value of the extract liquor to 8 to 10, delivering the extract liquor through a macroporous resin column, eluting the extract liquor with alcohol of different concentration, and obtaining extract extractum after recovering solvent with alcohol eluent. The purification by high-speed countercurrent chromatogram comprises taking petroleum ether, n-butyl alcohol and phosphate buffer as the solvent system to purify the extractum by a high-speed countercurrent chromatogram method, and obtaining the high-purity huperzine A.
Owner:LIAOCHENG UNIV

Amoxicillin sodium and sulbactam sodium for injection and preparation of freeze-dried injection thereof

The invention discloses a preparation method of amoxicillin sodium and sulbactam sodium for injection and a freeze-dried powder injection thereof as well as a separating and purifying method of the amoxicillin sodium and sulbactam sodium for injection. By adopting high speed countercurrent chromatography, the invention prepares trichloromethane, ethyl acetate, methanol and water to form a solvent system with an immobile phase and a mobile phase and separates and purifies amoxicillin sodium and sulbactam sodium to obtain the amoxicillin sodium and sulbactam sodium for injection; the purity of the obtained product can reach more than 98% and the prepared injection has improved stability.
Owner:海南华旗药业销售有限公司

Method and apparatus for counterconcurrent chromatography

A countercurrent chromatography apparatus includes a plurality of plates, at least (16) having first and second interleaved spiral flow channels (52, 54, 56, 58) therein. Each spiral flow channels (52, 54, 56, 58) has a first end (I1, I2, I3, I4) near the central axis and a second ends (O1, O2, O3, O4) near the periphery. The outlet of the first channel (O1) is connected to the inlet of the second channel (I2) by a connecting channel (72). Septa may be provided between the plates to connect the spiral channels of one plate to the spiral channels of the next plate.
Owner:US DEPT OF HEALTH & HUMAN SERVICES

Method for separating and purifying Momordica grosvenori leaf chromocor compound by high-speed countercurrent chromatography and products thereof

The invention discloses a method for separating and purifying a flavone compound of momordica grosvenori leaves by HSCCC and the product thereof, wherein, the method of the invention comprises the following steps: the grosvenori leaves are added with water or other polar solvents so as to carry out reflux extraction; the obtained extracts adopt the mixed solution of ethyl acetate, normal butanol and water as a solvent system and are separated and purified by a preparation-type high-speed counter current chromatograph, and then kaempferol-3-O-Alpha-L- rhamnose-7-O-[Beta-D-glucosyl group(1-2)-O-L-rhamnoside] and kaempferol-3,7-Alpha-L-2- rhamnoside. In the method, by applying the HSCCC method to separate and purify the extracts of the momordica grosvenori leaves, two flavone compounds with higher purity are successfully obtained by separation, thereby extending the source of the raw materials for flavone products; moreover, the method has simple preparation technique, easy operation and high purification efficiency, lays the solid first stone for extraction and utilization of the effective components in the momordica grosvenori leaves, and can avoid environment pollution caused by the waste and throw of a large amount of momordica grosvenori leaves.
Owner:GUANGXI NORMAL UNIV

Method for separating flavone compound from hericium erinaceus by applying high-speed countercurrent chromatography

The invention discloses a method for separating a flavone compound from hericium erinaceus by applying a high-speed countercurrent chromatography. The method comprises the following steps: 1) subjecting hericium erinaceus fermented mycelia to extraction by adopting high-purity ethanol, then carrying out filtration, carrying out pressure-reducing concentration so as to recover ethanol, and carrying out extraction with petroleum ether and ethyl acetate so as to obtain a crude extract; 2) mixing chloroform, dichloromethane, methanol and water according to a volume ratio of 4: 2: 3: 2 so as to obtain a mixed solution used as a two-phase solvent system, and carrying out standing and stratifying, wherein the upper phase is used as a stationary phase, and the lower phase is used as a mobile phase; and 3) carrying out separation through the high-speed countercurrent chromatography so as to obtain the flavone compound. The method is simple and convenient, has good reproducibility, can be used for large-scale preparation of compounds, and lays a material foundation for further research on activity.
Owner:ZHEJIANG UNIV OF TECH

Process for rapid preparation of high pure pharmaceutical matters from patrinia villosa juss

The invention provides a rapid preparation method of some high pure pharmaceutical matter of patrinia villosa juss. The process comprises : (1) extraction of medical materials; (2) preparation of crude extract; (3) separation and purification of obtained crude extract by high-speed countercurrent chromatography, comprising solvent system for stationary phase and mobile phase, filling stationary phase in countercurrent chromatographic column, running the column, pumping the mobile phase into column, injecting sample via injection valve, and receiving the demanded components according to the detection spectrogram, wherein, the said two phase solvent system is composed of n-hexane, ethyl acetate, methanol, water and ethyl acetate, n-butyl alcohol, water.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY

Process for extracting xanthohumol from lupulus

The invention discloses a method for extracting xanthohumol from hops, which comprises the following steps: firstly pulverizing hops, adding petroleum ether to degrease fully, extracting with ethanol, filtering, concentrating the filtrate under reduced pressure to obtain a thick extract, distributing the thick extract with ethyl acetate / water, concentrating the ester phase under reduced pressure, vacuum-drying to obtain crude hop xanthohumol extract, and further separating and purifying the crude hop xanthohumol extract by two-step high-speed counter-current chromatography to finally obtain high-purity xanthohumol. The method overcomes the disadvantages of the conventional separation method such as the irreversible absorption effect of a sample, thereby improving the sample recovery rate. The yield of xanthohumol prepared from hops is up to 0.17% or more, and the purity of the prepared xanthohumol is up to 98% or more.
Owner:SHANXI UNIV

Method for separating and purifying polydatin and resveratrol from traditional Chinese medicine giant knotweed

The invention discloses a separating and purifying method of black falsehellebore and veralkalcohol from traditional Chinese medicine giant knotweed rhizome, which comprises the following steps: 1. grinding giant knotweed rhizome; extracting alcohol through immersing method and ultrasound; separating the extract and drug slag; extracting drug slag through alcohol; combining the extract and first extract; separating to remove solid impurity; 2. adding extract on the big hole adsorption resin column; eluting through different density of alcohol to obtain extract I and extract II; 3. purifying extract I and extract II through high-speed reflow chromatography to obtain the product.
Owner:LIAOCHENG UNIV

Method for preparing lycium barbarum lutein by HSCCC (high-speed countercurrent chromatography) separation

The invention discloses a method for preparing lycium barbarum lutein by HSCCC (high-speed countercurrent chromatography) separation. The method mainly comprises the following steps: grinding and extracting lycium barbarum berry, concentrating extract, saponifying, eluting and dissolving, and carrying out HSCCC separation to obtain a lycium barbarum lutein solution; concentrating, and freeze-drying to obtain a lutein pure product of which the purity is over 90.6 percent by means of HPLC analysis and detection. According to the method, a methanol and tetrahydrofuran composite solvent is adopted for ultrasonic extraction, and a saponification technology is combined and synergized with HSCCC, so that the problem that main carotinoid lutein and zeaxanthine in lycium barbarum belong to isomers and are difficult to separate can be successfully solved; autumn berry of lycium barbarum is utilized as a raw material, so that the production cost is greatly reduced, effective separation of lutein and zeaxanthine can be successfully realized by repeatedly regulating the solvent proportion in the counter-current chromatography separating and purifying phase, a high-purity lutein monomer can be obtained, and a new thought is provided to implementation of separating and purifying lutein from lycium barbarum and implementation of industrial production.
Owner:NINGXIA ACADEMY OF AGRI & FORESTRY SCI

Method for extracting morroniside and loganin from Macrocarpium officinalis

The present invention relates to a method for extracting morroniside and loganin from Macrocarpium officinalis. The method comprises ultrasonically extracting with 70% ethanol, extracting with n-butanol, concentrating an extractive solution and recovering n-butanol to near dryness to obtain crude morroniside and loganin, adopting an n-butanol-ethanol-water system as a two-phase solvent system, dissolving the crude morroniside and loganin in a bottom phase, separating by high-speed countercurrent chromatography, concentrating eluates under a reduced pressure to near dryness, respectively, crystallizing with n-butanol and ethyl acetate for 2-3 times, respectively, filtering out crystal, and drying to obtain the final products. The method has a simple process and low cost. The products have high purity.
Owner:苏州宝泽堂医药科技有限公司

Apparatus for countercurrent chromatography

A plate apparatus for use in countercurrent chromatography is disclosed which comprises a disk shaped tube support (60) having a tube support pattern forme in an upper surface (63) of the support and configured to accommodate at leas one layer of fluid flow tubing (70), wherein the pattern comprises at least one spiral groove (62) and at least one return path (72).
Owner:HEALTH & HUMAN SERVICES THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC

Method for preparing flavonoid glycoside and stibene glucoside type compound by separating from fenugreek

InactiveCN103304605AEfficient separationOvercome the disadvantages of dead adsorption loss and low yieldSugar derivativesEther separation/purificationSeparation technologyRhapontigenin
The invention discloses a method for preparing a flavonoid glycoside and stibene glucoside type compound by separating from fenugreek. By virtue of a high-speed countercurrent chromatography (HSCCC) separation technology, isomerides such as vitexin and isovitexin and stibene glucoside type compounds such as rhaponticin, deoxyrhapontin and rhapontigenin which have the similar polarity to that of the isomerides in the fenugreek are subjected to separation preparation, so that the separation time is effectively shortened, and the shortcomings of complicated operation, sample die-adsorption loss, low yield and the like of the conventional preparation method are overcome. According to the method, the technology is simple, the reproducibility is high, and the separation efficiency is high; the obtained monomer content is higher than 96.0 percent and can meet the requirements of a type of botanical drugs and standard contrasts; and the method is suitable for industrial production of medical medicines, standard substances, health protection food, health protection medicines and the like.
Owner:湖州藏源生物科技有限公司

Method for separating and extracting Sirolimus from fermentation liquid

The invention provides a method for separating and extracting Sirolimus from fermentation liquid. The extraction process comprises the following steps of: preparing Sirolimus fermentation mycelia; performing extraction and decoloration; performing high-speed countercurrent chromatography separation; and drying. In the method, the multi-chromatography steps in the traditional extraction process are replaced by countercurrent chromatography separation technology; extraction is combined with countercurrent; and a Rapamycin product with purity of near 90 percent can be obtained by one-step countercurrent separation. Compared with the relatively complex traditional Rapamycin extraction process, the fermentation mycelia crude extraction and high-speed countercurrent chromatography method has the advantages of simple separation route, contribution to amplification of the process and suitability for quickly separating and preparing high-additional-value Rapamycin monomers from the fermentation liquid in large scale. The method for separating and extracting the Sirolimus from the fermentation liquid has the advantages of high yield, simple extraction steps, solvent saving and the like, and can be applied to industrial actual amplified production.
Owner:SOUTHWEST UNIV

Method for preparing high-purity taste-removing purple sweet potato pigment

The invention discloses a method for preparing a high-purity taste-removing purple sweet potato pigment, and belongs to the field of food additives and agricultural product deep processing. The method for preparing the high-purity taste-removing purple sweet potato pigment comprises the steps that 1, pretreatment is conducted on purple sweet potato raw materials; 2, the purple sweet potato pigment is extracted from low-frequency microwave auxiliary acidified aqueous solution; 3, preliminary purification is conducted on macroporous resins; 4, high-speed counter-current chromatography separation and purification are conducted, and the taste-removing purple sweet potato pigment with concentration more than 95% is obtained. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, the microwave auxiliary acidified aqueous solution is used for extraction, flocculants are added to flocculate mucoproteins, then macroporous resin static adsorption and dynamic elution are conducted, and the taste-removing and enrichment purification process is preliminarily accomplished, and the high-purity taste-removing purple sweet potato pigment is finally prepared through the high speed counter-current chromatography method in a separation mode. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, technological processes are advanced, separation efficiency is high, recovery rate is high, product purity is more than 95%, preparation amount is large, an obtained product is free of peculiar smell, has few impurities and is good in solubility, and method for preparing the high-purity taste-removing purple sweet potato pigment is easy to popularize and use.
Owner:NINGBO HAITONG FOOD SCI & TECH CO LTD +1

Mezlocillin sodium and sulbactam sodium for injection and freeze-dried injection preparation thereof

The invention provides a preparation method of mezlocillin sodium-sulbactam sodium for injection and the freeze-dried powder injection thereof as well as a separating and purifying method of the mezlocillin sodium and sulbactam sodium for injection. By adopting high speed countercurrent chromatography, the invention prepares trichloromethane, methanol and water to form a solvent system with an immobile phase and a mobile phase and separates and purifies mezlocillin sodium and sulbactam sodium to obtain the mezlocillin sodium and sulbactam sodium for injection; the purity of the obtained product can reach more than 99% and the prepared injection has improved stability.
Owner:海南华旗药业销售有限公司

Reciprocal Symmetry Plots as a New Representation of Countercurrent Chromatograms

InactiveUS20080201085A1Good and more reliablyAssess instrument performanceComponent separationChemical methods analysisAnalyteData set
Various methods for visualization of output from a liquid-liquid chromatographic instrument are provided. One or more analytes detected by a liquid-liquid chromatographic instrument are visualized by providing a data set comprising a plurality of data points corresponding to one or more analytes detected by the instrument, wherein the data points comprise at least one parameter related to a K-value or a parameter from which a K-value can be determined. A K-value is calculated for at least a portion of the data set, and at least a portion of those K-values transformed by a reciprocal transformation to generate output data having a transformed K-value, wherein the transformed K-value is a real number for all K undergoing the transformation, thereby ensuring that all analytes detected by the instrument are plotted in a single chromatogram. The output data is provided to a user. The output data may be used for instrument performance testing, design, calibration, or for selecting suitable solvent systems to detect analytes of interest.
Owner:THE BOARD OF TRUSTEES OF THE UNIV OF ILLINOIS

Preparation method for high-purity di-coffee mesitoyl quinine acid compounds

The invention discloses a method for preparing a high purity dicaffeoylquinic acid compound by high-efficient separation, mainly comprising: using an alcohol-water solution to extract taraxacum fresh plant or dry plant, carrying out the column chromatographic separation after the concentration, preparing a crude extract rich in dicaffeoylquinic acid compound, then isolating and purifying the crude extract by high-speed countercurrent chromatography, and finally adopting a recrystallization process for refining so as to obtain 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid with high purity. The method is applied to the preparation of high purity monomers by taking various natural products or natural product extracts containing one or a plurality of ingredients in the dicaffeoylquinic acid compound and acquired through various approaches as raw materials, and has advantages of simple steps, simple operation, high efficiency, low cost, easy repetition, and large output of separation and preparation. The purity of dicaffeoylquinic acid compound prepared by the method can reach 98 percent.
Owner:施树云 +1

Method for separating and purifying yam polysaccharide

InactiveCN103910809ANo antibacterial activityAntibacterial activity is not strongWater bathsCountercurrent chromatography
The invention discloses a method for separating and purifying yam polysaccharide. The method comprises such steps as pre-treating, extracting by hot water bath, removing protein by use of a Sevage method, roughly separating polysaccharide by use of high-speed counter-current chromatography and further purifying by use of a SephadexG-100 chromatographic column. In the method disclosed by the invention, the extraction process flow and process parameters of the yam polysaccharide are determined, the chromatographic conditions of the high speed counter-current chromatography and the SephadexG-100 purification of the yam polysaccharide are determined, the structure of the extracted yam polysaccharide is verified, and the antioxidant activity and antibacterial activity of the yam polysaccharide are measured.
Owner:ZHEJIANG GONGSHANG UNIVERSITY

Method for preparing lactuca indica and lactucin

The invention relates to a method for preparing lactuca indica and lactucin, which is a method for using two separation processes to obtain lactuca indica and lactucin from cichorium glandulosum by a high-speed countercurrent chromatography. The method has the advantages of large fractional dose, small sample loss, high recovery rate, mild separation environment and solvent saving. A great amountof lactuca indica and lactucin crude products can be placed in a countercurrent chromatograph, the separation result achieves the high purity and good separation effect. The method is not only suitable for preparing the product with high purity from plant crude extract, but also suitable for purifying lactuca indica and lactucin crude extracts obtained by various approaches.
Owner:XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI

High-speed countercurrent chromatographic method for separating terpene component in tree moss extract

ActiveCN104155395AConducive to accurate characterizationEasy to quantifyComponent separationAcetic acidStationary phase
The invention discloses a high-speed countercurrent chromatographic method for separating a terpene component in a tree moss extract. The method comprises the following steps: 1, mixing n-hexane with ethyl acetate, ethanol and water according to a volume ratio of 0.7-1.1:0.7-1.1:0.8-1.1:0.8-1.1, carrying out acute oscillation, and allowing the obtained mixture to stand in order to form two layers comprising an upper layer used as a stationary phase and a lower layer used as a mobile phase; 2, dispersing the tree moss extract by the mobile phase to obtain a sample solution to be separated; and 3, separating the sample solution through high-speed countercurrent chromatography, collecting a corresponding fraction according to an ultraviolet absorption map, and carrying out solvent removal on the obtained fraction to obtain the terpene component with the relative content of above 90%. The method has the advantages of mild conditions, high separation efficiency, good stability, simple process, and easy realization of popularization use and industrial production.
Owner:CHINA TOBACCO JIANGSU INDAL

Method for extracting and purifying tanshinone monomeric compounds from red sage root

InactiveCN102167721ALess polar impuritiesLow recovery temperatureQuinone separation/purificationSteroidsDichloromethaneCountercurrent chromatography
The invention discloses a method for extracting and purifying tanshinone monomeric compounds from red sage root, which comprises the steps of: (1) preparing an extract: smashing the red sage roots, immersing in dichloromethane, extracting by percolation, ultrasound or refluxing, and recovering solvent from an extracting solution to obtain the extract of the red sage root; and (2) purifying with high-speed countercurrent chromatography: purifying the obtained extract by the high-speed countercurrent chromatography with petroleum ether-n-butyl alcohol-methanol-water as a two-phase solvent system, so as to obtain seven monomeric compounds with purity over 95%, including dihydrotanshinone I, 1,2,15,16-tetrahydrotanshinquinone, cryptotanshinon, tanshinone I, neo-przewaquinone A, tanshinone II A and miltirone.
Owner:LIAOCHENG UNIV

Method for separating and extracting cannabidiol by high-speed countercurrent chromatography

The invention discloses a method for separating and extracting cannabidiol by high-speed countercurrent chromatography, belonging to the field of industrial hemp extraction. The method comprises the following steps: 1) drying and crushing industrial hemp flowers and leaves; 2) extracting the cannabidiol with methanol or ethanol to obtain a cannabidiol alcohol extract; 3) extracting with petroleum ether to obtain a petroleum ether phase, and removing the petroleum ether to obtain extract; 4) separating the extract by high-speed countercurrent chromatography, and collecting eluent; and 5) concentrating and crystallizing the eluent to obtain a cannabidiol product.
Owner:黑龙江康源生物科技有限公司

Method for separating active ingredients from aquilaria sinensis lamina by utilizing high-speed countercurrent chromatography

The invention discloses a method for separating active ingredients from an aquilaria sinensis lamina by utilizing a high-speed counter current chromatography. The method comprises the following steps of: extracting the dried and smashed aquilaria sinensis lamina by virtue of a percolation extract method at the room temperature to obtain an aquilaria sinensis lamina extracting solution extractum; extracting the extracting solution extractum for 3-4 times by sequentially utilizing petroleum ether,and ethyl acetate, respectively decompressing and concentrating a petroleum ether extract liquor and an ethyl acetate extract liquor to extractums; then respectively separating the petroleum ether part extractum and the ethyl acetate part extractum to obtain an active ingredient A of 7,4'-dimethoxy-5-hydroxy flavone by utilizing the high-speed counter current chromatography; and then separating the ethyl acetate part extractum through high-speed countercurrent chromatography to obtain an active ingredient B of 3,5,7,3',4'-pentahydroxy-flavone and an active ingredient C of 3,5,7,4'-tetrahydroxy-flavone after coarsely separating the ethyl acetate part extractum by virtue of a column chromatography on silica gel. The active ingredients separated by the method are low in loss, high in separation speed, high in purity, stable and easy to apply.
Owner:北京利科德科技有限公司

Method for enriching and purifying aloe polysaccharides in aloe

The invention belongs to the field of natural organic chemistry, and relates to a method for purifying aloe polysaccharides, particularly a preparation method for enriching and purifying aloe polysaccharides in aloe by a high-speed countercurrent chromatography separation process. The method is characterized by purifying natural plant aloe leaching liquor twice by a high-speed countercurrent chromatography separation process to obtain aloe polysaccharides of which the purity is higher than 95%. The method is suitable for preparing high-purity aloe polysaccharides from natural plants containing aloe polysaccharides and extracts of the natural plants. The method is suitable for preparing aloe polysaccharides by separating with various types of countercurrent chromatographs, and the aloe polysaccharides prepared by the high-speed countercurrent chromatography process can be continuously, efficiently and quickly separated by liquid-liquid partition chromatography; and thus, the method has the characteristics of great separation amount, no sample loss, high recovery rate, mild separation environment, solvent saving and the like.
Owner:DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products