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Method for separating purified pelargonidin anthocyanin monomer from rubus hirsutus

A technique for separation and purification of pelargonidin, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of undiscovered separation and preparation of geranidin-3-O-glucoside research and reports, and achieve the goal of overcoming Sample adsorption loss, large injection volume, good repeatability of continuous injection

Inactive Publication Date: 2017-06-13
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no research and report on the separation and preparation of geranidin-3-O-glucoside from pomegranate by high-speed countercurrent chromatography

Method used

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  • Method for separating purified pelargonidin anthocyanin monomer from rubus hirsutus
  • Method for separating purified pelargonidin anthocyanin monomer from rubus hirsutus
  • Method for separating purified pelargonidin anthocyanin monomer from rubus hirsutus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Wash 1000g of fresh pomegranates, add 70% ethanol solution containing 0.1% (v / v) hydrochloric acid according to the ratio of material to liquid ratio of 1g: 6mL, mix well, and ultrasonically extract for 90min (control the temperature below 45°C, Protect from light), vacuum pumping rate after the ultrasonic wave, the obtained filter residue was repeatedly extracted once according to the above conditions (changing the ratio of solid to liquid to 1g: 4mL), combined the filtrate, and removed ethanol by vacuum rotary evaporation at 40°C to obtain crude anthocyanin liquid;

[0029] (3) According to the ratio of crude anthocyanin extract: ethyl acetate volume ratio of 1:1, add ethyl acetate for extraction, let stand to separate, extract four times, collect and combine the water phase. Remove residual ethyl acetate by vacuum rotary evaporation at 40°C to obtain anthocyanin loading solution;

[0030] (4) The AB-8 macroporous resin soaked in ethanol for 24h is packed into a ...

Embodiment 2

[0038] Accurately weigh 5kg of fresh pomegranates, add 80% ethanol solution containing 0.5% (v / v) formic acid according to the ratio of material to liquid: 1g: 8mL, mix thoroughly, and extract by ultrasonic for 240min (control the temperature below 45°C, avoid light) , filtered with three layers of gauze after the ultrasonic wave, and the obtained filter residue was repeatedly extracted once according to the above conditions (changing the ratio of solid to liquid to 1g:2mL), combined the filtrate, and removed ethanol by vacuum rotary evaporation at 50°C to obtain the crude extract;

[0039] Add ethyl acetate for extraction according to the volume ratio of the crude anthocyanin:ethyl acetate volume ratio of 2:1, let stand to separate layers, extract four times, collect and combine the aqueous phases. Remove residual ethyl acetate by vacuum rotary evaporation at 50°C to obtain anthocyanin loading solution;

[0040] Put the AB-8 macroporous resin soaked in ethanol for 24 hours i...

Embodiment 3

[0043] Accurately weigh 10kg of fresh fruit, add 90% ethanol solution containing 1% (v / v) hydrochloric acid according to the ratio of solid to liquid: 1g: 10mL, mix thoroughly, and extract by ultrasonic for 240min (control the temperature below 45°C, avoid light) , the vacuum pumping rate after the ultrasonic wave is over, and the ethanol is removed by vacuum rotary evaporation at 60°C to obtain the crude extract of fenugreek anthocyanins;

[0044] According to the ratio of crude anthocyanin extract: ethyl acetate volume ratio of 1:1, ethyl acetate was added for extraction, the layers were left to stand, extracted six times, and the aqueous phases were collected and combined. Remove residual ethyl acetate by vacuum rotary evaporation at 60°C to obtain anthocyanin loading solution;

[0045] Put the AB-8 macroporous resin soaked in ethanol for 24 hours into a chromatographic column, wash it with water until there is no alcohol smell, and wash it with 4% HCL solution, deionized w...

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Abstract

The invention discloses a method for separating a purified pelargonidin anthocyanin monomer from rubus hirsutus. According to the method, high-purity pelargonidin-3-O-glucoside is obtained by separated and purificated from the rubus hirsutus through alcohol extraction concentration, ethyl acetate extraction, AB-8 macroporous resin adsorption and high-speed countercurrent chromatography, and the purity of the pelargonidin-3-O-glucoside is greater than 99 percent. By using the method, 113.3mg of pelargonidin-3-O-glucoside can be obtained from 1000g of fresh rubus hirsutus fruits; compared with a traditional separation method, the purity and yield thereof are improved; moreover, the process is simple, so industrialized production can be realized, and a new idea is provided for comprehensive development and utilization of rubus hirsutus resources of China.

Description

technical field [0001] The invention relates to the separation and purification of natural products, in particular to a method for separating and purifying geranium anthocyanin monomers from pomegranates. Background technique [0002] Anthocyanins are a class of water-soluble pigments that widely exist in fruits and vegetables. Different types of anthocyanins have different structures and colors, which endow fruits and vegetables with bright colors ranging from red, purple red to blue. In recent years, a large number of studies have confirmed that anthocyanins from natural fruits and vegetables have biological activities such as anti-oxidation, anti-tumor, prevention of cardiovascular diseases, alleviation of diabetes symptoms and obesity control. Pelargonidin 3-O-glucoside (Pg3G for short) is the most representative compound among anthocyanins and has excellent biological activity. Studies have shown that Pg3G has the functions of anti-oxidation and protection against oxid...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08
CPCC07H17/065C07H1/08
Inventor 陈卫徐阳鲍涛
Owner ZHEJIANG UNIV
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