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Method for separating and purifying polydatin and resveratrol from traditional Chinese medicine giant knotweed

A technology for the separation and purification of resveratrol glycosides, which is applied in the field of traditional Chinese medicine preparations and its preparation, can solve problems such as environmental hazards and troublesome operation, and achieve the effect of simple and fast operation and high purity

Inactive Publication Date: 2007-05-16
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The above method may require the use of halogenated hydrocarbons as solvents, which is a serious hazard to the environment, or requires repeated column chromatography, which is cumbersome to operate

Method used

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  • Method for separating and purifying polydatin and resveratrol from traditional Chinese medicine giant knotweed
  • Method for separating and purifying polydatin and resveratrol from traditional Chinese medicine giant knotweed

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Take 100 g of pulverized Polygonum cuspidatum medicinal material, add 1.5 L of 95% ethanol, soak at room temperature for 24 hours, heat and reflux for 3 hours, and separate the medicinal liquid. Add 1 L of 95% ethanol, heat to reflux for 2 hours, and separate the liquid medicine. The extracted medicinal liquids are combined, and the impurities in the extraction liquid are removed by filtering. The extraction solvent is recovered by vacuum distillation to obtain the ethanol extract of Polygonum cuspidatum.

[0032] Put the ethanol extract obtained above on a D101 macroporous adsorption resin column, wash it with water until it becomes colorless, elute it with 15% ethanol until it is colorless, collect the 15% ethanol eluate, and distill under reduced pressure to recover the solvent to obtain the extract I. It was then eluted with 20% ethanol until it was colorless, and the 20% ethanol eluate was collected, and the solvent was recovered by distillation under reduced pre...

Embodiment 2

[0035] Take 100 g of pulverized Polygonum cuspidatum, add 1 L of 95% ethanol, soak at room temperature for 10 hours, and ultrasonically treat for 60 minutes to separate the medicinal liquid. Add 1L of 95% ethanol, sonicate for 30min. Combine the extracted medicinal liquids, and filter to remove impurities in the extracted liquids. The extraction solvent is recovered by vacuum distillation to obtain the ethanol extract of Polygonum cuspidatum.

[0036] Put the ethanol extract obtained above on a D101 macroporous adsorption resin column, wash it with water until it becomes colorless, elute it with 15% ethanol until it is colorless, collect the 15% ethanol eluate, and distill under reduced pressure to recover the solvent to obtain the extract I. It was then eluted with 20% ethanol until it was colorless, and the 20% ethanol eluate was collected, and the solvent was recovered by distillation under reduced pressure to obtain extract II.

[0037]Using petroleum ether-ethyl acetat...

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Abstract

The invention discloses a separating and purifying method of black falsehellebore and veralkalcohol from traditional Chinese medicine giant knotweed rhizome, which comprises the following steps: 1. grinding giant knotweed rhizome; extracting alcohol through immersing method and ultrasound; separating the extract and drug slag; extracting drug slag through alcohol; combining the extract and first extract; separating to remove solid impurity; 2. adding extract on the big hole adsorption resin column; eluting through different density of alcohol to obtain extract I and extract II; 3. purifying extract I and extract II through high-speed reflow chromatography to obtain the product.

Description

1. Technical field [0001] The invention relates to a traditional Chinese medicine preparation and a preparation method thereof, in particular to a method for separating and purifying resveratrol glycosides and resveratrol from traditional Chinese medicine Polygonum cuspidatum. 2. Background technology [0002] Resveratrol glycosides and resveratrol have various biological effects beneficial to the human body, such as anti-tumor, anti-aging, lowering cholesterol, inhibiting platelet aggregation, and antibacterial. [0003] Polygonum cuspidatum is rich in resveratrol glycosides and resveratrol, and methods for extracting and separating resveratrol glycosides and resveratrol from Polygonum cuspidatum have been reported in the literature. [0004] Zhu Lixian [Research on the Extraction and Separation Technology of Resveratrol in Polygonum cuspidatum, Anhui Agricultural Sciences, Issue 01, 2005] Using single factor and orthogonal experiment to study the extraction conditions of r...

Claims

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Application Information

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IPC IPC(8): C07C37/68C07C39/21C07H1/08C07H15/18A61K36/704
Inventor 柳仁民孙爱玲
Owner LIAOCHENG UNIV
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