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Method for separating flavone compound from hericium erinaceus by applying high-speed countercurrent chromatography

A technology of high-speed countercurrent chromatography and flavonoids, which is applied in the field of separation and purification of flavonoids, can solve the problems of large amount of reagents, time-consuming, environmental pollution, etc., and achieve the effect of good reproducibility and simple method

Active Publication Date: 2017-06-20
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the separation of natural products is mainly carried out by column chromatography. This method is easy to cause environmental pollution, takes a long time, requires a large amount of reagents, and is easy to form irreversible adsorption on the sample.

Method used

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  • Method for separating flavone compound from hericium erinaceus by applying high-speed countercurrent chromatography
  • Method for separating flavone compound from hericium erinaceus by applying high-speed countercurrent chromatography
  • Method for separating flavone compound from hericium erinaceus by applying high-speed countercurrent chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The fermented mycelium powder of Hericium erinaceus in this example was purchased from Beijing Fuerkang Biology Co., Ltd.

[0033] 1. Preparation of sample material for high-speed countercurrent chromatography (HSCCC) (crude extract of fermented mycelium of Hericium erinaceus)

[0034] Get Hericium erinaceus fermented mycelium powder 1000g, use the ethanol of 95% mass concentration that is 20 times of weight of Hericium erinaceus fermented mycelium powder to reflux extract at 30 ℃ for 3 times, three extraction times are 8h, collect extract , concentrated by filtration, concentrated under reduced pressure, recovered ethanol, and obtained the extract. First extract with petroleum ether to remove the oily substance, then extract with ethyl acetate, collect the extract, concentrate under reduced pressure to obtain the crude extract of fermented mycelium of Hericium erinaceus. As a sample loading material for high-speed countercurrent chromatography.

[0035] 2. HPLC detec...

Embodiment 2

[0053] Get Hericium erinaceus fermented mycelium powder 1000g, use the ethanol of 95% mass concentration that is 20 times of weight of Hericium erinaceus fermented mycelium powder to reflux extract at 30 ℃ for 3 times, three extraction times are 8h, collect extract , concentrated by filtration, concentrated under reduced pressure, recovered ethanol, and obtained the extract. First extract with petroleum ether to remove the oily substance, then extract with ethyl acetate, collect the extract, concentrate under reduced pressure to obtain the crude extract of fermented mycelium of Hericium erinaceus. As a sample loading material for high-speed countercurrent chromatography.

[0054] HPLC detection method is the same as embodiment 1

[0055] For the separation and extraction of high-speed countercurrent chromatography, add chloroform, dichloromethane, methanol, and water (volume ratio: 6:4:5:3) into the separatory funnel, mix and static layer, ultrasonically degas, and separate t...

Embodiment 3

[0059] Get Hericium erinaceus fermented mycelium powder 1000g, use the ethanol of 90% mass concentration that is 15 times of weight of Hericium erinaceus fermented mycelium powder to reflux extract at 30 ℃ for 3 times, three extraction times are 12h, collect extract , concentrated by filtration, concentrated under reduced pressure, recovered ethanol, and obtained the extract. First extract with petroleum ether to remove the oily substance, then extract with ethyl acetate, collect the extract, concentrate under reduced pressure to obtain the crude extract of fermented mycelium of Hericium erinaceus. As a sample loading material for high-speed countercurrent chromatography.

[0060] HPLC detection method is the same as embodiment 1

[0061] For the separation and extraction of high-speed countercurrent chromatography, add chloroform, dichloromethane, methanol, and water (volume ratio: 4:2:3:2) into the separatory funnel, mix and static layer, ultrasonically degas, and separate ...

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Abstract

The invention discloses a method for separating a flavone compound from hericium erinaceus by applying a high-speed countercurrent chromatography. The method comprises the following steps: 1) subjecting hericium erinaceus fermented mycelia to extraction by adopting high-purity ethanol, then carrying out filtration, carrying out pressure-reducing concentration so as to recover ethanol, and carrying out extraction with petroleum ether and ethyl acetate so as to obtain a crude extract; 2) mixing chloroform, dichloromethane, methanol and water according to a volume ratio of 4: 2: 3: 2 so as to obtain a mixed solution used as a two-phase solvent system, and carrying out standing and stratifying, wherein the upper phase is used as a stationary phase, and the lower phase is used as a mobile phase; and 3) carrying out separation through the high-speed countercurrent chromatography so as to obtain the flavone compound. The method is simple and convenient, has good reproducibility, can be used for large-scale preparation of compounds, and lays a material foundation for further research on activity.

Description

technical field [0001] The invention relates to a method for separating and purifying flavonoids, in particular to a method for separating and purifying high-purity flavonoids in Hericium erinaceus by high-speed countercurrent chromatography. Background technique [0002] Hericium erinaceus belongs to the genus Hericium erinaceus. Its mature fruiting bodies and mycelia produced by fermentation can be used as raw materials of traditional Chinese medicine, with mild and stable properties and sweet and delicious taste. Its main components are pyrone, steroids, alkaloids, aromatics, and diterpenoids, which have the functions of anti-tumor and repairing nerve tissue. [0003] Modern studies have shown that flavonoids have various physiological functions and pharmacological effects such as anti-cancer, anti-oxidation, anti-inflammatory, anti-virus, and immune regulation. Their extracts can be used in food, medicine, cosmetics and other products. Genistein can inhibit colon cance...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40
CPCC07D311/36C07D311/40
Inventor 何晋浙樊鹏孙培龙
Owner ZHEJIANG UNIV OF TECH
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