The invention provides a bee pollen active probiotic beverage and a preparation method thereof. The bee pollen active probiotic beverage is made of bee pollen as a raw material, mixed with a certain proportion of maltose and / or galactose, mixed and fermented by two kinds of probiotics, Lactobacillus BL1 and Lactobacillus BL2, and then formulated. In view of the fact that the bee pollen raw material is highly acidic and difficult to be utilized by microorganisms, the present invention selects two strains with strong acid resistance and various excellent properties. By controlling the fermentation process, the number of viable probiotic bacteria in the product can be kept at 1011cfu / L within 30 days The above, and can biotransform bee pollen flavonoid glycosides to obtain a large amount of flavonoid aglycone, and improve the bioavailability of bee pollen flavonoids. At the same time, it is rich in other bee pollen and probiotic fermentation products. It is rich in nutrition, rich in fragrance, sweet and sour, and has the functions of beautifying and nourishing the skin, enhancing immunity, lowering cholesterol, regulating intestinal flora and preventing constipation.