A method for simultaneous separating total flavonol glycosides and
ginkgolides from
ginkgo biloba extract is provided. The method is characterized in that the
ginkgo biloba extract is taken as a
raw material,
column chromatography enrichment and purification through
polyamide and
macroporous resin are conducted, after eluent is concentrated, ester solution is added for extraction, an organic phase part is washed with water, standing is conducted for stratification, an aqueous phase and an organic phase are combined correspondingly, an
solvent is recovered,
drying and smashing are conducted, and then the high-purity total flavonol glycosides and the
ginkgolides are obtained. According to the method for simultaneous separating the total flavonol glycosides and the
ginkgolides from the
ginkgo biloba extract, it is guaranteed that the purity of the total flavonol glycosides simultaneously separated from the
ginkgo biloba extract reaches 90% or more, the transfer rate is not less than 65%, the purity of the total of the sum of ginkgolides A, ginkgolides B and ginkgolides C is more than 85% or more, the transfer rate of the sum of the ginkgolides is not less than 55%, and total
ginkgolic acid contained is less than 1mg / kg; organic extraction is combined with
water washing so that the high-purity total flavonol glycosides and the ginkgolides can be simultaneously obtained with rapid speed and low cost, residue organic solvents is low, and
sensitization substance is less or few.