365results about How to "Improve enrichment effect" patented technology

The invention discloses a bioremediation method for soil polluted by heavy metals. The bioremediation method comprises the following steps of: adding earthworm feeds into the soil polluted by the heavy metals, turning the soil, uniformly mixing, and putting earthworms into the soil and cultivating the earthworms; after the earthworms are cultivated for 28 to 35 days, collecting earthworm excrements in the upper layer soil, collecting adult earthworms by adopting a natural lighting method, and retaining young earthworms and earthworm eggs in the soil; planting plants by adopting a hedge planting method, cradling the parts of the plants growing for 78 to 90 days, which are positioned above the ground, and removing the whole plants after the plants grow for another 50 to 70 days; collecting the adult earthworms and the earthworm excrements for 1 to 3 days by adopting an outdoor collection method every 28 to 35 days during planting of the plants; and after the whole plants are removed, collecting all the adult earthworms and the young earthworms by adopting the natural lighting method, and collecting the earthworm excrements and the earthworm eggs. The soil is subjected to remediationby combining animals, plants and microorganisms according to a proper remediation sequence and a remediation period, so that the bioremediation method is economic, environment-friendly, easy to operate, high in remediation efficiency and easy to popularize, and is particularly suitable for mild or moderate copper, cadmium and lead combined polluted soils.

The invention discloses a method for recycling platinum group metal from waste catalysts. The method comprises the following steps that (1) the waste catalysts containing the platinum group metal, nickel type collecting agents, calcium oxide and silicon dioxide are crushed and mixed uniformly according to a proportion; (2) the proportioned mixture is smelted at the high temperature, and the constant temperature is kept for a period of time, so that molten sulfonium with nickel as main components is deposited below a slag layer of metallic oxide; (3) water cooling is conducted on the sulfonium at the high temperature, so that metal particles are obtained; (4) the crushed metal particles are leached through sulfuric acid at the high pressure, and valuable metal such as nickel is recycled from leaching liquid; and (5) the platinum group metal is further extracted from leaching slag. Compared with the prior art, the method is simple in development process, good in enrichment effect and capable of being effectively used for separation and extraction of valuable metal in the waste catalysts.

The invention provides an enriching method of circulating tumor cells (CTCs) and a kit thereof. The enriching method is applied to quickly and efficiently enriching CTCs from blood. The enriching method comprises the following steps of coupling an anti-epithelial cell adhesion molecule (EpCAM) antibody onto a magnetic nanosphere to prepare an immune-magnetic bead; adding the immune-magnetic bead into a blood sample for hatching for 5 minutes, and realizing separating, enriching and purifying of CTCs by the steps such as magnetic separating and washing. The enriching method disclosed by the invention is high in capturing efficiency, short in enriching time, good in specificity and very reliable. Activity ratio of the captured cell is kept at (90.5 + / -1.2)%, and the captured cell can be directly used for cultivation, reverse transcriptase polymerase chain reaction (RT-PCR) and immune cell chemical analysis. Besides, the enriching method provides powerful means for detecting and researching CTCs, and has extremely important medical significance.

The invention relates to a magnetic solid-phase extraction agent based on a covalent organic framework material and a preparation method and application thereof. The magnetic solid-phase extraction agent comprises a ferriferous oxide magnetic nanoparticle core, a polydopamine layer and a covalent organic framework material COF-5 layer, and the polydopamine layer and the covalent organic framework material COF-5 layer are sequentially coated outside the core. A method includes: adding functionalized magnetic nanoparticles into a to-be-treated glycoprotein sample, sufficiently stirring for extraction, using a magnet to separate the magnetic nanoparticles from a solution, adding elution liquid to elute off the glycoprotein from the magnetic nanoparticles, and measuring. The covalent organic framework material can selectively enrich glycoprotein and effectively separate the glycoprotein from impurities, so that the method has good extraction effect on the glycoprotein. The solid-phase extraction method is simple in operation step, short in time, high in recycling rate, low in organic solvent consumption and suitable for enrichment and measurement of the glycoprotein in complex biological samples like plasma and urine.

The invention relates to a method for analyzing carbon isotopes of micro single hydrocarbons in natural gas. The method is characterized by utilizing a divinyl benzene / carbon molecular sieve / polydimethylsiloxane (DVB / CAR / PDMS) composite adsorbing material to enrich the microcomponents in the hydrocarbons, carrying out thermal desorption at the gas chromatography injection port and carrying out separation through gas chromatography and analyzing the carbon isotopes of the single hydrocarbons by isotope ratio mass spectrometry. The method has the following beneficial effects: the method has the advantages of high carbon isotope testing precision and good repeatability, conforms to the research needs, is simple to operate and has good use and popularization values.

A preparation method of a thermosensitive microgel asymmetric supported nano silver catalyst comprises the steps of preparing poly-(N-isopropylacrylamide-styrene) / poly-(N-isopropylacrylamide-methacrylic acid) core-shell microgel by a soap-free emulsion polymerization method and a seed emulsion polymerization method, and then synthesizing the asymmetric supported nano silver catalyst taking the microgel as a template by a pickering emulsion template method. The thermosensitive microgel asymmetric supported nano silver catalyst prepared by the method shows good thermosensitivity, and good catalytic activity as the catalytic reaction efficiency is regulated and controlled by temperature and the thermosensitivity of the microgel simultaneously during catalytic reduction of p-nitrophenol.

The invention discloses a method of quickly repairing a cadmium polluted cultivated land by crop rotation of a cadmium super-enriching plant and an energy plant. The method comprises the following step: planting the cadmium super-enriching plant and the energy plant in the cadmium polluted cultivated land in the natural growth periods thereof for conventional cultivation to achieve the purpose ofrepairing the cadmium polluted cultivated land by means of adsorbing and enriching abilities of the cadmium super-enriching plant and the energy plant, wherein the cadmium super-enriching plant is sedum plumbizincicola and the energy plant is sorghum or oilseed rape. The invention provides a planting mode which is not limited by growth seasons. Based on one year as the repair period, the soil repair efficiency of the polluted cultivated land is increased by means of a relatively long growth period in multi-cropping plantation, and the method is short in repair period, high in operability and low in cost, and repairs the land more thoroughly.

The invention discloses a method for preparing pneumocandin B0. The method comprises the following steps of: a) centrifuging the fermentation liquid of echinocandin B0, taking mycelium, leaching the echinocandin B0 in the mycelium with a first solvent and filtering and removing the mycelium; b) distilling the solvent in the first solvent leaching solution of echinocandin B0 to dryness, soaking with a second solvent and then filtering and removing the insoluble substances; c) distilling the second solvent soak solution of echinocandin B0 to dryness and then dissolving in the first solvent, andcollecting effluent liquid through overly acidic alumina column; d) distilling the collected solution of echinocandin B0 to dryness and then dissolving in the first solvent, utilizing adsorbent resin,performing eluting with the first solvent and then collecting the part with higher purity; e) distilling the collected solution of echinocandin B0 to dryness and then dissolving in the first solvent,utilizing inverse resin, performing eluting with the first solvent and collecting the part with higher purity; and f) distilling the collected solution of echinocandin B0 to dryness and then dissolving in the first solvent, adding in a small amount of water by dripping to achieve the purpose of separation by crystallization after supersaturation, and then preparing the echinocandin B0. The methodfor preparing the echinocandin B0 not only can well remove the pigment, but also leads the purity of the echinocandin B0 to be improved by more than 96 percent.

The invention relates to a biochip for enriching and detecting circulating tumor cells and preparation method thereof. The invention comprises the following steps: placing a clean substrate on flame, obtaining a cigarette ash layer composed of nano cigarette ash particles on surface of the substrate by deposition, using the nano cigarette ash particle as template, and deposing a layer of silica housing layer on external surface of nano cigarette ash particles by chemical vapor deposition method; removing nano cigarette ash particles in the silica housing layer by high temperature calcining, and obtaining a silicon dioxide layer composed of nano silica particles with micrometer thickness; finally, modifying an anti-EpCam antibody which can specifically identify tumor cells on the surface of the silicon dioxide layer, thereby obtaining a biochip for enriching and detecting circulating tumor cells. The biochip can enrich circulating tumor cells with high efficiency and high sensitivity, and the product has high transparency under water and can be used for detecting real time captured circulating tumor cells.

The invention discloses a method and a reagent kit for constructing BRCA 1 / 2 (breast cancer susceptible genes 1 / 2) detection libraries. The method includes carrying out fragmentation and tail-end repair on homogenized DNA (deoxyribonucleic acid) samples in DNA fragmentation and tail-end repair systems under the effects of interruption enzymes and tail-end repair enzymes; linking products obtained at the previous step with linker sequences under the effects of DNA ligase; utilizing linker linking products obtained at the previous step as templates and carrying out PCR (polymerase chain reaction) amplification by the aid of primers of linker sequences under the effects of DNA polymerases; hybridizing probe sequences with BRCA 1 / 2 and products obtained at the previous step to trap DNA fragments with the BRCA 1 / 2 and carrying out elution to obtain hybridization trapped products; carrying out cyclization on single strands of the hybridization trapped products under the effects of ligase to obtain the BRCA 1 / 2 detection libraries. The method and the reagent kit have the advantages that steps for constructing the libraries are simple, convenient and speedy, the cost can be effectively reduced, workload can be relieved, and variation types are comprehensive and accurate and are high in flux.

The invention belongs to the technical field of electroanalytical chemistry and specifically discloses a novel ionic liquid 4-hydroxy-1-methyl-1-(3-pyrrole propyl)-piperidine bromine salt and an electrochemical sensor based on an ionic liquid-graphene oxide composite nanometer material modified electrode. According to the invention, interface characteristics of the modified electrode are inspected by AC (alternating current) impedance spectroscopy and electrochemical behaviors of honokiol on the modified electrode are researched by voltammetry. As shown by the result, honokiol has a pair of reversible redox peaks on the modified electrode. Compared with a bare glassy carbon electrode, the modified electrode has the advantages that the peak current of the redox peaks of the honokiol on the modified electrode is greatly reinforced, a good linear relation is built between the peak current and honokiol of which the concentration is between 3.0*10<-8> and 1.0*10<-5> mol.L-1, and the detection limit is low. The electrochemical sensor prepared by the invention is successfully applied to the detection of honokiol in traditional Chinese medicine cortex magnoliae officinalis, so that the industrial prospect is good.

The invention discloses a solid phase microextraction fiber for polycyclic aromatic hydrocarbon enriching detection and a manufacturing method thereof, and belongs to the technical field of contaminant detection. The invention provides a method capable of being used for manufacturing a solid phase microextraction coating on different fiber substrates and realizing combined use with gas chromatography or gas chromatography-mass spectrum, and the method is used for polycyclic aromatic hydrocarbon contaminant detection. Metal fiber is used as a substrate; dopamine is polymerized on the surface ofthe substrate so as to provide functional groups for polydopamine; the polydopamine is firstly combined with COF material single ligands so as to provide an anchor point; a PDA-COP composite layer isfinally synthesized and is used for solid phase microextraction. The solid phase microextraction fiber has the advantages that through the great pi bond effects between the solid phase microextraction fiber and the polycyclic aromatic hydrocarbon, the adsorption effect on the polycyclic aromatic hydrocarbon can be enhanced; during the enrichment, a certain separation effect is achieved; the substrate pretreatment step of samples can be effectively simplified; meanwhile, the enriching capability of the polycyclic aromatic hydrocarbon contaminants can be greatly improved; the detection limit ofthe polycyclic aromatic hydrocarbon contaminants can be reduced.

The invention discloses a method enabling lucid ganoderma and conidial powder of the lucid ganoderma to be rich in microelements. The method at least includes the following measures that firstly, dedicated lucid ganoderma substitute cultivation culture media are prepared; secondly, special moisturizing water is prepared and sprayed so that moisturizing can be achieved; thirdly, cut-log cultivation is adopted; fourthly, cut-log cultivation is adopted, but when the third measure is not executed, microelement nutrient solutions are diluted by clear water by 300-500 times, then serve as the special moisturizing water and are sprayed to lucid ganoderma in the growing period for conducting moisturizing for 2-3 times according to a conventional method, wherein spraying is carried out every five to seven days; fifthly, the second measure or the fourth measure should be repeatedly executed in cultivation and management for the lucid ganoderma and the conidial powder of the lucid ganoderma rich in microelements in the second crop and / or the third crop.

The invention discloses a method for processing heavy metal Cd contaminated soil, which is characterized by comprising the steps of adding rhamnolipid aqueous solution with concentration of 0.5-2g / L into a negative electrolytic tank, adding deionized water into a positive electrolytic tank, installing a filter cloth between the Cd contaminated soil needed to process and the negative electrolytic tank as well as the positive electrolytic tank, installing a permeable reaction wall on an enrichment place of the heavy metal Cd far away from five eighth of an anode S3, and switching on to electronically repair the Cd contaminated soil. The Cd concentration rate is above 4 times higher than an initial value; and under the function, the concentration rate is reduced to about 3.63 times, the total removal ratio of the heavy value Cd of the whole soil is about 40%, and the contribution ratio of the permeable reaction wall on the removal ratio is about 90%, so that the alkaline rhamnolipid solution proves that the process of the heavy metal from enrichment to removal can be realized by the electrical repair and the permeable reaction wall. The method is simple in operation condition, wide in range of application, low in cost, high in efficiency, liable to realize, and has the better social benefit and biological benefit on the heavy metal Cd contaminated soil.

The invention discloses a method enabling shiitake mushrooms to be rich in microelements. The method at least includes the following measures that firstly, dedicated shiitake mushroom substitute cultivation culture media are prepared; secondly, special moisturizing water is prepared and sprayed so that moisturizing can be achieved; thirdly, cut-log cultivation is adopted; fourthly, cut-log cultivation is adopted, but when the third measure is not executed, microelement nutrient solutions are diluted by clear water by 300-500 times, then serve as the special moisturizing water and are sprayed to shiitake mushrooms in the growing period for moisturizing for 2-3 times according to a conventional method, wherein spraying is carried out every five to seven days; fifthly, the second measure or the fourth measure should be repeatedly executed in cultivation and management for the shiitake mushrooms rich in microelements in the second crop and / or the third crop.

The invention relates to a method for separating and enriching low-grade adamantane compounds in petroleum and hydrocarbon source rocks, which comprises the following steps: extracting a hydrocarbon source rock sample with dichloromethane and methanol solution to obtain a hydrocarbon source rock extract; removing normal saturated hydrocarbons and asphaltene components in the hydrocarbon source rock extract or crude oil sample by using a ZSM-5 molecular sieve, passing through silicagel columns to obtain isomeric saturated hydrocarbon components, adding beta-cyclodextrin solution into the isomeric saturated hydrocarbon solution, and separating the suspension to obtain a precipitate; moving the precipitate into a hydrochloric acid solution, putting into a baking oven at 40-95 DEG C, and keeping the temperature; and after the acid-catalysis hydrolysis reaction of the beta-cyclodextrin finishes, cooling to room temperature, and extracting with a saturated hydrocarbon solvent to obtain the low-grade adamantane compounds. By using the method, the recovery rate of the single-adamantane compounds can maximally reach higher than 18%, the recovery rate of the double-adamantane compounds can maximally reach higher than 35%, and the recovery rate of the tertiary-adamantane compounds can maximally reach higher than 1.5%.

The invention relates to a super-hydrophilic micro-well sensing interface for enrichment and trace detection and a preparation method of the super-hydrophilic micro-well sensing interface. The method comprises the following steps: repeatedly translating a clean substrate above candle flame at constant speed, carrying out physical deposition on the surface of the substrate to obtain uniformly-distributed carbon nanolayers with a certain thickness, carrying out chemical vapor deposition on silicon dioxide by taking the substrate as a template, and thus obtaining a nano-composite structure with carbon particles being coated by the silicon dioxide; calcining at high temperature to remove carbon cores, and thus obtaining a uniform hollow nano silicon dioxide layer with a micron thickness; after treating the surface of nano silicon dioxide by a plasma, modifying a silanization reagent on the surface by adopting a monomolecular-film self-assembly method; covering a circular photomask plate, and carrying out ultraviolet light degradation on the silanization reagent in the uncovered area by adopting a photoetching technique to obtain the super-hydrophilic micro-well sensing interface for enrichment and trace detection. By utilizing the advantage of a controllable patterning designated limited range, a super-hydrophilic micro-well has good concentration and enrichment effects on micro droplets of an extremely dilute solution and can be used for real-time trace detection of target molecules.

The invention relates to a method of the smelting technical field, in particular to a method for extracting yellow gold from gold extracting residue and complex and unmanageable hard-to-deal electrum. The method successively comprises the following steps of: (1) proportioning raw materials in the following parts by weight to prepare mixed materials; (2) putting the mixed materials prepared in thestep (1) into an oxygen enrichment side-blown bath for melting; (3) discontinuously releasing copper matte produced in the step (2) from the oxygen enrichment side-blown bath to be blown into yellow gold by the oxygen enrichment side-blown bath; and (4) carrying out flotationon melt residue and blowing residue, and returning the flotation concentrate used as concentrate residue to step (1). The invention aims to solve technical problems that gold extracting residue and electrum difficult to deal are difficult to deal, and are hard to be sufficiently used. The invention has advantages of: (1) a great quantity of copper concentrate or a small quantity of copper concentrate is mixed to enter the oxygen enrichment side-blown bath to be melted to form yellow gold, so the recovery rate of gold and silver is improved; (2) copper matte has favorable enriching performance, and valuable elements in the copper matte can be recovered.

The invention provides a surface enhanced Raman scattering substrate, which comprises a polylactic acid electrostatic spin film and nano metal particles on the surface or in the inner of the polylactic acid electrostatic spin film, wherein the nano metal particles carry positive charges. The surface enhanced Raman scattering substrate has the advantages of high enhancing ability, good repeatability, long storage period, and capability of enriching articles to be detected. The invention also provides a preparation method of the surface enhanced Raman scattering substrate. The preparation method comprises the following steps: (1) dissolving polylactic acid into an organic solvent to obtain a spinning liquid, and using the spinning liquid to carry out electrostatic spinning to obtain a polylactic acid electrostatic spin film; (2) arranging nano metal particles on the surface and in the inner of the obtained polylactic acid electrostatic spin film through a spotting mode so as to obtain the surface enhanced Raman scattering substrate. The preparation method is simple and is suitable for massive production. The prepared surface enhanced Raman scattering substrate can be applied to food analysis, environment analysis, etc.

The invention discloses a detecting method for trace amount aroma components in a natural aromatic product. The detecting method mainly comprises steps of: puffing and trapping pre-treatment, puffing and trapping of volatile components, thermal desorption cold hydrazine system enrichment components, GC-MS analyzing and the like. The detecting method enriches substances of the natural product by a sample pretreatment technique-puffing and trapping / thermal desorption; compared with the traditional static headspace, the puffing and trapping technique can analyze volatility and semi-volatile compound with high molecular weight, has stronger analytical capacity on the identification of olefine, is particularly suitable for analyzing trace amount terpene compound with volatility components such as essential oil and the like, does not need using a dissolvent for extraction, can cause experimenters not to expose in potential hazardous chemicals and does not need to carry out waste treatment on the dissolvent. The detecting method has simple operation, high automation degree, environmental friendly, good enrichment effect and high sensitivity.

The invention discloses functional resin containing 4,6-dimercapto-1,3,5-triazine alkali metal salt and a preparation method thereof. The method comprises the steps of dripping ethanediamine into chloromethylated bead mixture swelled by a solvent to carry out nucleophilic substitution to obtain the resin containing active amino; carrying out substituted grafting reaction on the obtained resin containing the active amino and cyanuric chloride to obtain the resin grafted with the cyanuric chloride; carrying out sulfhydrylation reaction on the prepared resin grafted with the cyanuric chloride in water solution of Na2S and / or NaSH, and then alkalifying with aqueous alkali after the sulfhydrylation reaction is finished. The method is simple in processing equipment, and convenient and simple to operate; and the sulfydryl resin prepared by the method is high in sulfydryl content, large in adsorbing capacity on heavy metal ions, strong in adsorption capacity, strong in regeneration capacity, good in repeated use effect, free of pollution, and low in cost, can remove and gather metal ions of heavy metals such as copper, lead, nickel, chromium, mercury and the like in waste water at the same time, and especially has good absorption efficiency when the concentration of the heavy metals in the waste water is low.

The invention relates to the field of aquatic product detection, and discloses a method for detecting the heavy metal content of an aquatic product. The method comprises the following steps: 1), pretreating: removing inedible inner organs, fish skin, fish scales, fish bones and other hard tissues of the aquatic product, then crushing remaining tissues into minced meat, adding an aqueous Triton X-100 solution and a magnetic heavy metal adsorbent, mixing and stirring; 2), performing heavy metal extraction; 3), calibrating a standard curve; 4), performing heavy metal content detection. By the heavy metal detection method, strong acid and a microwave digestion instrument are not required to be used for digestion, operation is simple, safety and environment friendliness are achieved, the effectof enriching heavy metals in the aquatic product is good, the magnetic heavy metal adsorbent can be reused, remaining impurities of the aquatic product have no influence on a detection sample duringthe detection, and a detection result is accurate, so that the heavy metal detection method is a very accurate, efficient and environment-friendly detection method and has a practical significance inimproving the food safety.

The invention belongs to the technical field of nano materials, and provides a hydrothermal preparation method and application of a magnetic ferroferric oxide nanochain and provides the magnetic ferroferric oxide nanochain synthesized in the presence of amine surfactants in one step. The magnetic ferroferric oxide nanochain prepared by the method has good absorption to heavy metal ions Cr (VI) and can be used for water treatment. The method provided by the invention is simple in operational step, and the magnetic ferroferric oxide nanochain prepared is stable in repeatability, good in dispersibility, larger in specific surface area and non-toxic, can exist in different acid-base environments stably, and has a good absorption effect to heavy metal ions (especially hexavalent chromium ions).

The invention discloses a method for step recycling of iron, copper and noble metal from copper smelting waste slag. The method comprises the following steps: firstly, respectively crushing and treating copper smelting waste slag, a reducing agent carbon powder and an alkali substance caustic soda and a sodium salt anhydrous sodium sulfate into certain granularity, sufficiently and uniformly mixing the raw materials, pelletizing, adding alkali and performing reduction roasting at a high temperature, wherein the functions of the caustic soda and the anhydrous sodium sulfate are to reduce the viscosity of the slag, and effective aggregation of copper particles in the slag and sufficient enrichment of an iron component to magnetite phase are facilitated; after roasting is accomplished, performing magnetic selection to obtain magnetic concentrate (iron-copper component); and subsequently performing Nelson reselection to separate the copper and the noble metal in the magnetic concentrate. The magnetic tailings can be applied to the building industry and high-quality abrasive preparation. According to the method, the caustic soda and the anhydrous sodium sulfate are innovatively added, and Nelson reselection is adopted, so that step recycling of noble metal in the copper smelting waste slag is achieved, the method has the advantages of simplicity and convenience in process, high applicability, low production cost, no emission and the like, a novel method is provided for comprehensive utilization of the copper smelting waste slag, and remarkable economic benefits and social benefits are achieved.

The invention relates to a preparation method for eggs rich in omega-3 polyunsaturated fatty acids. The preparation method comprises the following steps of: (1) adding screened rubber seed kernel or rubber seed oil cake into non-gm feed for laying hens, with the additive amount of the rubber seed kernel being 10-20% of the total weight of the feed for laying hens, and the additive amount of the rubber seed oil cake being 15-25% of the total weight of the feed for laying hens; (2) adjusting ingredients of the feed to enable the feed to comprise the following ingredients: 42.89 of corn, 17-32 of soybean meal, 0.59 of wheat bran, 2-3 of lard, 10-20 of rubber seed kernel or 15-25 of rubber seed oil cake, 0.35 of salt, 8.38 of stone powder, 1.8 of calcium hydrogen phosphate, 0.1 of lysine, 0.14 of methionine, 1 of premix for laying hens, and 5 international units of vitamin E per kg of feed; (3) feeding 130-140-day laying hens by using the feed for laying eggs rich in omega-3 polyunsaturated fatty acids after feeding for 2-4 weeks. The preparation method is low in cost, stable in content of the omega-3 polyunsaturated fatty acids, and safe without peculiar smell, and effectively and sufficiently utilizes rubber seed resources.

The invention relates to a headspace gas chromatography detection technology, particularly discloses a vapor headspace enrichment detection method for a trace amount of volatile organic pollutants in seawater, and belongs to the technical field of water environment detection. The method sequentially comprises the four steps of water sample collection and storage, primary enrichment condensation, secondary enrichment condensation and headspace gas chromatography detection. According to the method, water vapor is used as sweeping gas, water is used as an absorbent, a water sample is secondarily distilled and condensed, and the condensed sample is enriched for headspace gas chromatography detection; a vapor headspace enrichment method is adopted, so that the using amount of an organic solvent in a process of enriching the volatile organic pollutants in the seawater is effectively reduced, and the interference of other components to a target object is prevented; an extraction process of the enrichment method is simple and convenient, the introduction of other solvents is avoided, the method is environment-friendly, low in limit of detection and high in recovery rate and reproducibility, and the requirements of detection of a trace amount of volatile organic pollutants in the seawater can be met.

The invention relates to the technical field of preparation of an environmental material, in particular to a yeast surface atom transfer imprinted adsorbent, and a preparation method and application thereof. The preparation method comprises the following steps of: through the atom transfer radical polymerization process, firstly, loading an initiator into a yeast surface to obtain a matrix material with the initiator; and then preparing the yeast surface imprinted adsorbent by using cefalexin as template molecules, using methacrylic acid (MAA) as a functional monomer, using ethylene glycol dimethacrylate (EGDMA) as a cross linking agent and using CuCl as a catalyst. The spherical imprinted adsorbent has obvious thermal and magnetic stability. The research on the effect between the template molecules and the functional monomer by ultraviolet rays shows that the acting force exists. A static adsorption experiment is used for researching the adsorption equilibrium, the dynamics and the selective recognition performance of the prepared imprinted adsorbent. A result shows that the yeast surface imprinted adsorbent obtained by utilizing the preparation method disclosed by the invention has high adsorption capacity, high-speed adsorption dynamics property and obvious cefalexin molecular recognition performance.

The present invention provides a dispersive liquid-liquid microextraction method for pretreatment of plant hormone, i.e. DLLME is used for pretreatment of plant hormone. The method comprising the steps of: loading, mixing an extracting agent and dispersing agent, oscillating to form an opacifying system, centrifugal separation and drawing the organic phase to perform HPLC analysis directly. The method of the invention has high enrichment property, simple operation, less use of organic solvent and short extraction time, integrates the sampling, extraction and concentration, at the same time, avoids the potential problem of cross contamination in solid-phase microextraction, is a novel sample pretreatment technique with the advantages of simpleness, rapidness, low cost, high efficiency and environment protection, and has broad application prospect in the field of trace analysis.

The invention relates to a preparation method for a hydrophilic magnetic nano material for the enrichment of glycopeptides, belongs to the fields of nano materials and bio-separation, and aims at achieving the selective enrichment of the glycopeptides. The method is characterized in that a one-pot method is used for synthesizing carbohydrates, such as chitosan, cellulose and agarose, and the hydrophilic magnetic nano material compatible with ferroferric oxide, and due to the existence of -NH2 and -OH polar groups in carbohydrate molecules in the material, the material is has the certain hydrophilicity, and the glycopeptides can be specifically enriched and purified in complex hydrolyzed proteins. According to the method, good adsorption effects of the prepared enrichment material are ensured; in addition, the preparation method has the advantages of time saving, low cost, convenience in operation and the like, and is particularly applied to the large-scale enrichment of the glycopeptides, and a large amount of material can be prepared.