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Dispersive liquid-liquid microextraction method for pretreatment of plant hormone

A liquid-liquid, plant hormone technology, applied in the field of analytical chemistry, can solve the problems of easy cross-contamination, high price of commercial columns, long column passing time, etc., and achieve the effect of reducing interference

Inactive Publication Date: 2010-06-30
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, SPE still has shortcomings such as expensive commodity bars and long time to pass bars
In 2007, solid-phase microextraction (SPME) was first used for the pretreatment of plant hormones. This method integrates sampling, extraction, and concentration, and is simple, fast, and environmentally friendly. However, SPME exists Expensive extraction head, short service life, easy cross-contamination and other limitations

Method used

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  • Dispersive liquid-liquid microextraction method for pretreatment of plant hormone

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Embodiment

[0032] The invention is used to measure the content of indole acetic acid (IAA) in chlorella.

[0033] Accurately weigh 0.5 g of the fresh sample of the plant to be tested, quickly grind it into powder with liquid nitrogen, and soak it in 80% methanol aqueous solution with 3 mL of 1 mmol / L antioxidant 2.6-di-tert-butyl-p-cresol BHT at 4 ° C. Extract overnight; centrifuge at low temperature, collect the supernatant, and extract the residue with 1 mL containing 1 mmol / L antioxidant 2.6-di-tert-butyl-p-cresol BHT at a volume concentration of 80% for another hour, combine the supernatants twice, and dilute After 5 times, the sample solution to be tested was obtained.

[0034] Add 5.0mL sample solution (pH 4.0) and 0.375g NaCl to a 10mL centrifuge tube with a stopper, mix 50.0μL chloroform and 1.0mL acetone, quickly inject the mixture into the sample solution with a syringe, gently Shake for 0.1-1min to form an emulsion system. Centrifuge at 4500r / min for 3 min, use a micro liqui...

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Abstract

The present invention provides a dispersive liquid-liquid microextraction method for pretreatment of plant hormone, i.e. DLLME is used for pretreatment of plant hormone. The method comprising the steps of: loading, mixing an extracting agent and dispersing agent, oscillating to form an opacifying system, centrifugal separation and drawing the organic phase to perform HPLC analysis directly. The method of the invention has high enrichment property, simple operation, less use of organic solvent and short extraction time, integrates the sampling, extraction and concentration, at the same time, avoids the potential problem of cross contamination in solid-phase microextraction, is a novel sample pretreatment technique with the advantages of simpleness, rapidness, low cost, high efficiency and environment protection, and has broad application prospect in the field of trace analysis.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a dispersion-liquid-liquid micro-extraction method for pretreatment of plant hormones. Background technique [0002] Phytohormones refer to some trace organic substances that are synthesized in plants and can have a significant effect on growth and development. Auxin is the earliest discovered plant hormone, which can regulate various physiological processes such as plant elongation growth, apical dominance, fruit development and so on. Establishing a sensitive, rapid and accurate analytical method for auxin is very important for elucidating the role and function of auxin in the process of plant growth. However, the content of hormones in plants is low (usually in the range of 0.1-50ng / g fresh weight), and there are many phytohormone analogs and secondary metabolites, which bring difficulties to the identification and quantification of phytohormones. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/40G01N30/74
Inventor 张兰卢巧梅陈丽惠陈国南
Owner FUZHOU UNIV
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