415 results about "Solid Phase Micro Extraction" patented technology

This invention relates to one method to test pyrazine compound in alcohol in liquor taste study, which applies top fix phase micro extraction and gas phase spectrum and flame thermal ion test device and uses gas phase spectrum and flare thermal ion test device cross technique in liquor with strong abnormal property of compound with good linear relation for R2>0.99 and compound test limit less than 1 mu g/L; Repeating the test relative standard bias for less than 15 percent and recycle rate larger than 80 percent.

The invention provides a simple and rapid preparation method and application of a covalent-organic framework compound solid-phase microextraction coating. According to the preparation method, the covalent-organic framework compound coating is quickly formed on quartz fiber, a device needed for the entire synthesizing process is extremely easy, and the method comprises the steps of performing amino-functionalization on the quartz fiber, impregnating surface amino-functionalized quartz fiber in a ground material precursor, repeating performing impregnating for several times, performing simple baking and cleaning the coating, so that the covalent-organic framework compound solid-phase microextraction coating can be obtained. The synthesizing method of the invention is easy to operate, requires few devices, and does not need an organic solvent, and by using the method, the covalent-organic framework compound solid-phase microextraction coating with high-temperature resistance, solvent resistance, acid and alkali resistance, high specific surface area, controllable pore size, and controllable thickness can be quickly prepared. Then the extraction coating is used to enrich environmentalpollutants polychlorinated biphenyls in water and it is detected that the coating has good enriching effects by using gas chromatography-mass spectrometry, thereby showing that the coating has good application prospect in the environment and other fields.

The invention discloses a method for quantificationally detecting phenolic compounds in solid-state fermentation products and belongs to the technical field of analysis of solid-state fermentation products. The method is mainly suitable for determination of the content of phenolic compounds in solid-state fermentation products of liquor, wherein the phenolic compounds includes yeast for preparing hard liquor, fermented grains and cellar mud. The invention particularly relates to ultrasonic extraction, headspace solid-state-phase micro-extraction and gas chromatography-mass spectrogram combined qualitative and quantitative analysis of solid-state fermentation products. The method built by the invention can be used for quickly detecting 10 kinds of volatile phenolic compounds in the solid-state fermentation products; the phenolic compounds have a favorable linear relationship R2 is more than 0.99 within a detection range; all detection limits are less than 10microgramme/L; the relative standard deviation is less than 10%; and the recovery rate is 75%-110%. The technical scheme provided by the invention is simple, convenient and quick, can be used for achieving effective monitoring of the volatile phenolic compounds in the solid-state fermentation products, and has the great significance in improving the quality of final products.

The invention discloses a solid phase micro-extraction device and a method for measuring volatile organic acids in tobacco leaves. The extraction device comprises a solid phase micro-extraction sample injection needle (1) and a headspace sample bottle (4), wherein a glass inner tube (5) is arranged inside the headspace sample bottle (4), a headspace bottle cover (2) is arranged at the mouth of the headspace sample bottle (4), the solid phase micro-extraction sample injection needle (1) is inserted into the headspace sample bottle (4) through the headspace bottle cover (2), and a solid phase micro-extraction fiber head (30 is mounted at the tip of the solid phase micro-extraction sample injection needle. The measurement method comprises the following steps of: weighing a tobacco leaf sample into and putting in the headspace sample bottle, adding internal standard compounds and anhydrous sodium sulfate, thoroughly mixing, sucking derivatization reagents into the glass inner tube, putting the glass inner tube into the headspace sample bottle, sealing the headspace sample bottle with the headspace bottle cover, and performing full automatic headspace solid phase micro-extraction. Compared with the prior art, the method has a simple treatment process and a high analysis speed, and is economical and practical, convenient to operate and environment-friendly.

The invention relates to an electrically-driven solid phase micro-extraction method and a device thereof. An integrated porous material prepared through treating an epoxy resin as a base material, diethylenetriamine as a curing agent and polyethylene glycol as a pore forming agent and utilizing an in-situ gradual polymerization process is adopted as a solid phase micro-extraction material, the form of the solid phase micro-extraction material to prepare a solid micro-extraction column is combined with a rotation-speed-adjustable motor, a stirring effect is realized through rotation, and simultaneously the solid phase micro-extraction material which is adopted as an electrical adsorption work electrode and a platinum wire electrode which is adopted as a counter electrode are combined with a high-voltage direct current power supply to realize an electric adsorption effect in order to complete the electrically-driven solid phase micro-extraction. The electrically-driven solid phase micro-extraction method combining the electric adsorption technology with the stirring technology effectively overcomes a disadvantage that magnetic stirring cannot be used in a small-volume solution and simultaneously reduces a disadvantage that the abrasion of a solid phase micro-extraction stirring bar is easy, so the service life can be prolonged; the integrated epoxy resin based porous polymer is treated as the adsorption material, so the extraction amount and the detection sensitivity are improved. The method has the advantages of simplicity, easy operation and high flexibility.

The invention discloses a covalent organic framework compound solid phase micro-extraction fiber head and a preparation method thereof, and belongs to the technical fields of analytical chemistry andfood safety detection. A solid phase micro-extraction fiber made from a covalent organic skeleton compound is prepared, and is used to prepare the solid phase micro-extraction fiber head, and the fiber head is used for solid phase micro-extraction. The solid phase micro-extraction fiber having a covalent organic framework (COF) material coating on the surface has the characteristics of large specific surface area, porosity, large pi-pi conjugate system and large thermal stability, so the COF material has a remarkable enriching effect on aromatic compounds. The fiber head can be applied to headspace solid phase micro-extraction of volatile target substances from extraction solutions with complex matrix and direct extraction of extraction solutions with a clean matrix, and also can be applied to detection after being combined with a gas chromatograph or a gas chromatograph/mass spectrometer.

A method to detect volatile component in yellow wine belongs to technical field of yellow wine specialty analysis. The invention adopts coupled technique of roof solid phase extraction and gas phase chromatography-mass spectrum to make qualitative and semi-quantitative analysis of volatile component in yellow wine. The method can detect volatile compound in yellow wine quickly and estimate its proportion in all volatile compound initiatively. Roof solid phase extraction can complete under normal temperature without organic solvent, which avoid producing new compound during analyzing and enrich volatile component effectively. Mass spectrum detection can identify volatile component accurately to improve accuracy of the method. The invention has significance in researching micro constituent in yellow wine and improving its quality.

The invention discloses a detection method for a volatile flavor substance of an edible vegetable oil. The method comprises the following steps: (1) putting samples into a headspace bottle, preheating at 55-65 DEG C for 15-20 minutes, inserting an aged solid-phase micro-extraction head into the headspace bottle, adsorbing for 40-50 minutes, and then inserting the extraction head into a gas chromatography sampling opening, analyzing at 250-260 DEG C for 3-5 minutes, carrying out volatile flavor substance extraction and enrichment on the detected samples of the edible vegetable oil; and (2) determining chromatographic conditions, wherein the chromatographic column is 30m*0.25mm*0.25mu m of J&W DB-5MS UI capillary chromatography column; the temperature of the sampling opening is 250 DEG C; the chromatographic column is splitless; a carrier gas is high-purity helium; and the flow rate is 1.0mL/min; carrying out temperature programming; according to chromatographic condition, injecting the prepared samples into a gas chromatograph-mass spectrometer to separate and detect, and obtaining components and relative content of the volatile component of the edible vegetable oil according to the determined original chromatographic data and mass spectrometric data. The method disclosed by the invention is simple in sample pretreatment, and free of a solvent, and qualitative and quantitative analysis of the volatile flavor substance of the edible vegetable oil can be rapidly achieved.

This invention relates to a capillary column solid phase coated with extraction phase micro extraction method and the device used with the analysis device, which comprises the following: one capillary solid phase micro extraction column with inner wall coated with solid phase, one six-way valve, extraction column heating box and transmission tube with desorption sample, micro three-way valve, micro air-extracting pump and assistant gas. The sample enters the extraction column under the assistant gas and micro air-extracting pump. After the flow and extracting of the sample, it rotates the six-way valve and immediately starts extraction column heating box to rapidly heat the capillary solid phase micro extraction column.

The invention discloses a method for preparing a solid phase micro-extraction fiber by self-assembling a graphene coating on a metal wire serving as a carrier layer by layer, and particularly relates to a method for self-assembling graphene on the surface of a metal wire layer by layer by taking gold nano particles as connection arms. The novel method is characterized by comprising the following steps of: performing chemical silver plating on the surface of the metal wire by taking the metal wire as the carrier; and self-assembling sulfydryl functional graphene on the silver-plated metal wire layer by layer by adopting a layer-by-layer self-assembly method and taking the gold nano particles as the connection arms, and thus obtaining the novel solid phase micro-extraction fiber by self-assembling the graphene coating layer by layer. The prepared solid phase micro-extraction fiber has the characteristics of high mechanical strength, stable extraction coating, high enrichment capacity, long service life and the like, can be used for enrichment analysis of trace components in samples, such as foods, environments, medicaments, biochemical substances and the like, and has high application potential.

The present invention provides a carbon nanotube solid phase microextraction extraction head and method for preparing. The carbon nanotube solid phase microextraction extraction head includes a stainless steel fibre and a surface coating layer coating on one end of the stainless steel fibre, characterized in that the surface coating layer has a carbon nanotube. The application of the carbon nanotube can make the solid phase microextraction extraction head fire stand, have high mechanical strength, and longer service life and high enrichment times; the invention also relates three manufacturing method of the carbon nanotube solid phase microextraction extraction head, a carbon nanotube solid phase microextraction extraction head having selectively strong absorption ability to polar material or non-polar material is prepared by the selectively applied field.

The invention relates to a novel solid phase micro-extraction stainless steel fiber and a preparation method thereof, which provides a method for easily and rapidly preparing the solid phase micro-extraction stainless steel fiber with high stability and high reproducibility. The preparation method is as follows: soaking the part of cleaned stainless steel wire into acid for treatment; washing by water; ageing under the protection of nitrogen, obtaining the solid phase micro-extraction stainless steel fiber; mounting the prepared solid phase micro-extraction stainless steel fiber in a microliter syringe, obtaining a simple solid phase micro-extraction device. The method is simple in operation and low in cost. The prepared solid phase micro-extraction stainless steel fiber has excellent extraction performance on all trace amount organic pollutants. Compared with commercial solid phase micro-extraction device, the solid phase micro-extraction device has better mechanical strength and thermal stability, and has low cost and long service life. The extraction fiber has excellent application potential in fields of environmental monitoring, food hygiene and the like.