837results about How to "Small amount of sample" patented technology

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen / antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen / antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

The invention provides a test method for trypsin. The test method comprises the following steps that: the fluorescence intensity of a metal nanocluster is tested; the trypsin reacts with the metal nanocluster, so that a reaction product is obtained, and the fluorescence intensity of the reaction product is tested; the difference between the fluorescence intensity of the metal nanocluster and the fluorescence intensity of the reaction product is calculated, and according to the difference and a predetermined standard curve, the content of the trypsin is obtained. The test method provided by the invention adopts the metal nanocluster as a fluorescence probe, the metal nanocluster comprises protein and metal, the property of the trypsin in fluorescently quenching the metal nanocluster is utilized, moreover, the degree of fluorescence quenching is positively correlated to the concentration of the trypsin, and the fluorospectrophotometry is adopted to determine the content of the trypsin. The test method provided by the invention has the advantages of high accuracy, high sensitivity and high selectivity, and an experimental result shows that the sensitivity of the test method provided by the invention on the test of the trypsin is 1.0 multiplied by 10 negative 9g / mL.

The invention discloses a method for quickly testing the appropriate granulation moisture content of an iron ore sinter mixture. The appropriate granulation moisture content refers to the moisture content required by the granulated mixture for obtaining optimal breathability. The moisture includes the moisture of the raw materials and the added water during mixing and granulation. The method comprises the following steps: 1) preparing a slurry mixture of which the humidity is 10 to 20 percent from iron ore, solvent, coke powder and sinter return ore; 2) rinsing and sieving the mixture by using saturated lime water as a medium, obtaining adhesive powder and shell grains and testing the content Xa of the adhesive powder; 3) testing the inner hole water-holding capacity of the shell grains by centrifugation; 4) testing the saturated water-holding capacity of the adhesive poser by using saturated water absorption process; and 5) calculating the appropriate moisture content required for the granulation of the mixture according to a formula. The method has the characteristics of simple operation and high accuracy. Based on an absolute error range from -0.3 to +0.3, the accuracy of the prediction of the appropriate moisture content reaches 93.3 percent according to the results of the tests of the moisture content of mixtures prepared by 30 material mixing schemes.

The invention relates to detection technology for element contents, and provides a method for simultaneously determining contents of arsenic, cadmium, lead, vanadium, zinc, manganese and phosphor in soil or deposits. The method mainly comprises the following steps: (1) 0.1g of a grinded drying soil sample is accurately weighed and placed in a TFM microwave digestion pot, a certain proportion of aqua regia and hydrofluoric acid are added, and a solution is allowed to stand; (2) an appropriate microwave digestion program is set, and microwave digestion is carried out for the solution in the step (1); (3) after the microwave digestion program ends, an acid-driving device is used for heating and evaporating the digestion solution in the step (2) to a dry state, and total transfer, volumetric flask preparation, and filtering are carried out; (4) ICP-OES is used for determining contents of arsenic, cadmium, lead, vanadium, zinc, manganese and phosphor in the clear liquor in the step (3); (5) ICP-MS is used for determining contents of elements in the clear liquor in the step (3) which do not reach detection limits in the step (4) once more. The method has the advantages of simple steps, simplified operation, saved soil samples, reduced personal error, minimized environment interference, and improved working environments.

The invention discloses an immunochromatography kit for fluorescence quantitative detection of salbutamol and a preparation method of a fluorescence labeling liquid. The kit comprises a test strip and a fluorescence labeling liquid, wherein the test strip is formed by sequentially lapping and sticking a sample pad, a nitrocellulose coating film and absorbent paper on a bottom plate; the nitrocellulose coating film comprises a detection area and a quality control area; the detection area is coated with an SAL-BSA conjugate; the quality control area is coated with anti-rabbit IgG; and the fluorescence labeling liquid contains a fluorescence labeling SAL antibody and a fluorescence labeling rabbit IgG. Compared with an immune colloidal gold labeling test strip, the kit disclosed by the invention has the advantages of higher sensitivity, accurate quantification and the like; and the operation is faster and more convenient than that of an enzyme coupling method.

The invention discloses a method for quickly evaluating the taste of bayberry juice through an electronic tongue system. The method comprises the steps of constructing a bayberry juice taste scoring and electronic tongue response signal relevance model, verifying the bayberry juice taste scoring and electronic tongue response signal relevance model, acquiring taste fingerprint information of a bayberry juice sample to be detected through the electronic tongue system, and calling the taste fingerprint information into the verified bayberry juice taste scoring and electronic tongue response signal relevance model to obtain a taste evaluation forecast value of the bayberry juice sample. The method for quickly evaluating the taste of the bayberry juice through the electronic tongue system is high in forecast precision and easy to operate and cannot be affected by external factors; compared with the conventional taste evaluation method, the method disclosed by the invention is high in reproducibility, objective, accurate and time-saving and is a new method for evaluating the taste of the bayberry juice.

The invention provides a preparation method of a multifunctional surface enhanced raman scattering (SERS) substrate. The preparation method comprises the following steps of: firstly, paving a layer of ZnO seeds on the inner wall of a capillary, developing a ZnO nanorod array in Zn(NO3)2 and hexamethylenetetramine (HMT) solution, and soaking and reacting the capillary in solution of (NH4)2TiF6 and H3BO3, thus obtaining a TiO2 nanotube array; and soaking a nanotube in which TiO2 is grown into SnCl2 to ensure that a layer of bivalent tin ions are adsorbed on the surface layer of the nanotube, soaking the nanotube into Ag(NH3)2+ and reducing the nanotube to silver particles, thus obtaining the multifunctional SERS substrate. Compared with the conventional SERS substrate, the multifunctional SERS substrate based on the capillary has functions of raman enhancement and repeated use, is convenient to carry and sample, and can realize real-time field detection.

The invention relates to the field of textile materials, and aims at providing a method for identifying a bamboo hemp fiber by using a terahertz time-domain spectroscopy technique. The method comprises the following steps of: respectively mixing and pressing a fiber with polyethylene powder to prepare a disc sheet after preparing the powder from the fiber as a guide sample; testing by adopting a transmission-type terahertz time-domain spectroscopy test device, thus respectively obtaining terahertz pulse time-domain waveforms through each guide sample; calculating the absorption coefficient and the refractive index of each guide sample, so as to respectively draw out an absorption spectrum and a refraction spectrum of the fiber; obtaining an absorption coefficient and a refractive index of the fiber to be identified; and confirming the type of the fiber to be identified according to the corresponding relation with the absorption spectrum and the refraction spectrum of each guide sample. According to the method, required sample quantity is simple, samples are simple and convenient to prepare, test time is short, and chemical pollutants are not caused in a test process; the absorption spectrum and the refraction spectrum of the sample at a terahertz waveband can be obtained at the same time when the sample is tested; the feature information of the samples is increased; the reliability of a test result is also improved.