346 results about "Rotavirus" patented technology

Double-stranded cDNA was synthesized from nucleic acid extracted from Norwalk virus purified from stool specimens of volunteers. One clone was isolated from a cDNA library constructed in a pUC-13 vector after amplification of the cDNA. The specificity of this cDNA (pUCNV-953) was shown by hybridization assays. The cDNA reacted with post (but not pre-) infection stool samples from Norwalk volunteers and with highly purified Norwalk virus, but not with other common enteric viruses such as hepatitis A virus and rotavirus. Finally, the probe detected virus in the same fractions of CsCl gradients in which viral antigen was detected using a specific Norwalk virus radioimmunoassay, and particles were detected by immune electron microscopy. Single-stranded RNA probes derived from the DNA clone after subcloning into an in vitro transcription vector were also used to show that the Norwalk virus contains a ssRNA genome of about 8 kb in size. The original clone was also used to detect additional cDNAs which represent at least 7 kb of nucleic acid of the Norwalk genome. The availability of a Norwalk-specific cDNA and the first partial genome sequence information allow rapid cloning of the entire genome and of establishment of sensitive diagnostic assays. Such assays can be based on detection of Norwalk virus nucleic acid or Norwalk viral antigen using polyclonal or monoclonal antibodies to proteins expressed from the cDNA or to synthetic peptides made based on the knowledge of the genome sequence. Assays using proteins deduced from the Norwlk virus genome and produced in expression systmes can measure antibody responses. Vaccines made by recombinant DNA technology are now feasible.

Methods for aiding in the diagnosis of disorders including, but not limited to, PDDs (Pervasive Development Disorders), Dysautonomic disorders, Parkinson's disease and SIDS (Sudden Infant Death Syndrome). In one aspect, a diagnosis method comprises analyzing a stool sample of an individual for the presence of a biological marker (or marker compound) comprising one or more pathogens, which provides an indication of whether the individual has, or can develop, a disorder including, but not limited to, a PDD, Dysautonomia, Parkinsons disease and SIDS. Preferably, the presence of one or more pathogens is determined using a stool immunoassay to determine the presence of antigens in a stool sample, wherein such antigens are associated with one or more pathogens including, but not limited to, Giardia, Cryptosporidium, E. histolytica, C. difficile, Adenovirus, Rotavirus or H. pylori.

Antimicrobial compositions that provide enhanced immediate and residual anti-viral and antibacterial efficacy against rhinovirus, rotavirus, coronovirus, respiratory syncytial virus, Gram-positive bacteria, Gram-negative bacteria and combinations thereof. More specifically, antimicrobial compositions comprising an organic acid or organic acid mixture and a short-chain anionic surfactant having at least one of a large head group; a branched alkyl chain and an unsaturated alkyl chain. Further, products incorporating the antimicrobial compositions of the present invention and methods of using the antimicrobial compositions and products are disclosed herein.

The invention discloses a multiple RT-PCR primer group for simultaneously detecting porcine gatahvirus (GETV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and porcine A rotaviruses (PoRV), which has a nucleotide sequence as shown in SEQ ID NO:1-SEQ ID NO:10. The invention further discloses a multiple RT-PCR detection method for detecting GETV, PEDV, TGEV, PDCoV and PoRV from a sample in one time by utilizing the multiple RT-PCR primer group. Compared with an existing conventional RT-PCR, the detection method has strong specificity and high sensitivity, can realizesimultaneous identification of five viruses including GETV, PEDV, TGEV, PDCoV and PoRV, and has accurate detection result and high detection efficiency.

The invention discloses a Chinese medicine preparation for treating infant rotavirus enteritis. The Chinese medicine preparation comprises the following raw materials in parts by weight: 10-20 parts of Chinese pulsatilla root, 10-20 parts of purslane, 10-20 parts of yam, 10-20 parts of baical skullcap root, 10-20 parts of wrinkled gianthyssop herb, 10-20 parts of radix glehniae, 10-20 parts of bighead atractylodes rhizome, 10-20 parts of Indian buead, 10-20 parts of burnet, 10-20 parts of longstamen onion bulb, 10-20 parts of kudzu vine root, 10-20 parts of coptis, 10-20 parts of dried tangerine peel, 10-20 parts of nutmeg, 10-20 parts of pomegranate rind, 10-20 parts of malt, 10-20 parts of grifola, 10-20 parts of ginger and 10-20 parts of endothelium corneum gigeriae galli. The preparation has the effects of relieving pain, regulating vital energy, tonifying the spleen, nourishing the spleen, the lung and the kidney, tonifying middle-Jiao and Qi, ascending yang and stopping diarrhea, and is mainly used for treating diarrhea caused by invasion of exogenous evil and weakness of spleen and stomach. The Chinese medicine preparation has the advantages of simple manufacture technology, small toxic side effect, convenience for administration, easiness for manufacture and low cost. The preparation can directly act on the focus, requires short curative time and avoids relapse after rehabilitation.

The invention discloses a kit for detecting pig epidemic diarrhea viruses, pig transmissible gastroenteritis viruses and pig rotaviruses. The kit comprises primer pairs shown by SEQ ID NO:1-2, SEQ ID NO:3-4 and SEQ ID NO:5-6 for respectively performing specific amplification on the pig epidemic diarrhea viruses, the pig transmissible gastroenteritis viruses and the pig rotaviruses. The invention also discloses applications of the primer pairs shown by the SEQ ID NO:1-2, the SEQ ID NO:3-4 and the SEQ ID NO:5-6 in preparation of reagents for detecting the pig epidemic diarrhea viruses, the pig transmissible gastroenteritis viruses and the pig rotaviruses. The detection kit disclosed by the invention can be used for accurately and effectively detecting the pig epidemic diarrhea viruses, the pig transmissible gastroenteritis viruses and the pig rotaviruses, and is strong in specificity, high in sensitivity, short in time consumption, rapid in detection and good in application prospect.

This invention provides formulations and methods for stabilizing viruses in liquid and dried formulations. In particular, formulations are provided including Zn2+ cations that stabilize the viability of Rotaviruses. Methods of vaccination include neutralization of gastric contents and administration of the vaccine formulations of the invention.

A vaccine composition and method of vaccination are provided useful for immunizing a subject against a rotavirus. The vaccines include rotavirus strains CDC-9 and CDC-66, fragments thereof, homologues thereof, or combinations thereof. Inventive vaccines may include a fragment of CDC-9, CDC-66, homologues thereof, or combinations thereof. Methods of inducing an immunological response are provided by administering an inventive vaccine.

A substituted Norovirus capsid protein monomer, having only the P-domain and called an antigen-Norovirus P-domain monomer, includes a foreign antigen inserted into one or more of three surface loops present on each P-domain monomer by molecular cloning. The antigen-P-domain monomer can assemble spontaneously into an octahedral form, called an antigen-Norovirus P-particle, that is composed of 24 copies of the antigen-P-domain monomer. Each substituted P-domain monomer will contain one to three copies of the foreign antigen, for a total of 24-72 antigen copies on each antigen-P-particle. The antigen-P-particle is useful in methods for diagnosing, immunizing and treating individuals infected with a foreign virus, for example Rotavirus, and can serve as a carrier for presentation of foreign antigens for development of novel vaccines against many infectious and non-infectious diseases. The substituted Norovirus P-particles can be readily produced in E. coli and yeast, are highly stable and tolerate a wide range of physio-chemical conditions. A modified Norovirus P-domain monomer includes one or more restriction recognition sites inserted within one or more of the three loops of the P-domain monomers, to provide user-friendly cloning cassettes for conveniently inserting candidate foreign antigens into the surface loops. The P-particle-VP8 chimeras may also serve as a dual vaccine against both rotavirus and norovirus.