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31 results about "Bovine coronavirus" patented technology
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Bovine coronavirus (BCV) is as a member of the Coronaviridae family which are enveloped, single-stranded, positive-sense RNA viruses with a club-shaped surface. Infection causes 'calf enteritis' and contributes to the 'enzootic pneumonia complex' in calves. It can also cause 'winter dysentery' in adult cattle. It can infect both domestic and wild ruminants and has a worldwide distribution. Transmission is horizontal, via oro-fecal or respiratory routes.
Multiplex rt-pcr/pcr for simultaneous detection of bovine coronavirus, bovine rotavirus, cryptosporidium parvum, and escherichia coli
InactiveUS20050026144A1Rapid and sensitive and specificRapid and sensitive and detectionSugar derivativesMicrobiological testing/measurementBovine rotavirusEscherichia coli
The present invention provides a multiplex RT-PCR / PCR method, which enables in a single assay the simultaneous detection of any combination of bovine rotavirus, bovine coronavirus, Cryptosporidium parvum, and optionally, Escherichia coli strains producing K99 pili or heat-stable enterotoxin STa.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
Bovine coronavirus chimeric antigen and aurosol immunochromatographic assay test strip for detecting bovine coronavirus antibody
ActiveCN110894217ASolve the problem of rapid diagnosis of diarrheaTroubleshooting Quick Diagnosis IssuesSsRNA viruses positive-senseVirus peptidesAntigenImmuno detection
The invention relates to the technical field of immunodetection, and discloses a bovine coronavirus chimeric antigen and an aurosol immunochromatographic assay test strip for detecting a bovine coronavirus antibody. The sequence of the chimeric antigen is shown as SEQID NO:1. The invention provides a novel bovine coronavirus chimeric antigen. When being used as a detection antigen of the bovine coronavirus antibody, the bovine coronavirus chimeric antigen has high specificity, repeatability and accuracy, and can be applied to associated immunochromatographic assay detection products. The results are visual and can be judged through naked eyes, the bovine coronavirus antibody in serum can be quickly and specially detected, and the problem for quick rapid diagnosis of diarrhoea of calves ina scaleable milk cow farm is solved. The bovine coronavirus chimeric antigen has high use value, and grass-roots unit promotion and application are facilitated.
Owner:CHINA AGRI UNIV
Kit, primer and probe for simultaneously detecting bovine viral diarrhea virus, bovine rotavirus and bovine coronavirus
ActiveCN112094953ASimple and fast operationStrong specificityMicrobiological testing/measurementMicroorganism based processesBovine rotavirusBovine Viral Diarrhea Viruses
The invention relates to the field of virus detection, and particularly relates to a kit, a primer and a probe for simultaneously detecting bovine rotavirus, bovine rotavirus and bovine coronavirus. The nucleotide sequences of the primer and the probe in the kit are shown as SED ID NO: 1-SEQ ID NO: 9. The kit has the technical advantages of being easy and convenient to operate, high in specificity, high in sensitivity, good in repeatability and capable of simultaneously achieving qualitative detection and accurate quantification of BVDV, BRV and BCoV.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY
Recombinant multi-epitope antigen of bovine coronavirus and application thereof
ActiveCN107033250AAvoid infectionImprove prokaryotic expression efficiencySsRNA viruses positive-senseBacteriaMolecular ImmunologyAgricultural science
The invention discloses a recombinant multi-epitope antigen of bovine coronavirus, also discloses application of the recombinant multi-epitope antigen of the bovine coronavirus in bovine coronavirus prevention ad diagnosis and belongs to the field of molecular immunology. An amino acid sequence of the recombinant multi-epitope antigen of the bovine coronavirus is a concatemer of a bovine coronavirus BCoV S protein epitope. The recombinant multi-epitope antigen, capable of causing a neutralizing antibody, of the bovine coronavirus serves as an immunogen or a vaccine, the neutralizing antibody oriented to the bovine coronavirus BCoV can be produced after the animal body is immunized, and the BCoV can be neutralized in vitro to prevent the animal body from virus infection. In addition, the recombinant multi-epitope antigen of the bovine coronavirus can also serve as a reagent for detecting a bovine coronavirus BCoV antibody or a polypeptide antibody.
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI +2
Inactivated bovine scours vaccines, processes and method of preventing bovine scours
InactiveUS6974577B2Prevention and control of diseaseBacterial antigen ingredientsSsRNA viruses positive-senseBacteroidesDisease
Inactivated scours vaccines for immunization and protection of bovine animals from disease caused by infection with bovine rotavirus and bovine coronavirus, which comprise and effective amount of at least one inactivated viral strain are described. Polyvalent inactivated vaccines further comprising an effective amount of an antigenic component which is protective against one or more additional pathogenic organisms or viruses are also disclosed. Said vaccines are prepared from one or more strains of rota- and coronavirus, C. perfringens Type C bacteria and E. coli bacteria, and combinations thereof. Preferably, a polyvalent inactivated vaccine is provided for parenteral administration. Passive immunity is achieved in neonatal calves via immunization of pregnant cows prior to birth.
Owner:ELANCO TIERGESUNDHEIT AG
Bovine rotavirus, coronavirus, and viral diarrhea virus multi-connected RT-PCR detection method
ActiveCN110079637AShorten the timeReagent savingMicrobiological testing/measurementAgainst vector-borne diseasesBovine rotavirusElectrophoresis
The invention discloses a bovine rotavirus, coronavirus, and viral diarrhea virus multi-connected RT-PCR detection method, which is characterized in that according to the detection method, RNA of a to-be-detected sample is taken as an RNA template, and a specificity upstream primer and a specificity downstream primer designed according to hereditary characteristics of the bovine rotavirus, coronavirus, and viral diarrhea virus are taken as a primer to perform RT-PCR proliferation to obtain a proliferation product. Then, electrophoresis detection is performed on the proliferation product with 1.5% agarose gel, and a result is recorded; according to the results of the electrophoresis detection, whether the to-be-detected sample contains the bovine rotavirus, coronavirus, and viral diarrhea virus is determined. The bovine rotavirus, coronavirus, and viral diarrhea virus detection method achieves has the beneficial effect of accurately, rapidly and efficiently distinguishing and diagnosingthe three viral disease with RT-PCR.
Owner:INNER MONGOLIA UNIV FOR THE NATITIES
Bovine coronavirus VPN (virtal private network) gene, encoded recombinant protein and application thereof
InactiveCN108715857ASimple and fast operationLow costSsRNA viruses positive-senseVirus peptidesPrivate networkElisa method
The invention discloses a bovine coronavirus VPN (virtal private network) gene, encoded recombinant protein and an application thereof. A BCoV (bovine coronavirus) indirect ELISA (enzyme linked immunosorbent assay) method is established by using the recombinant protein. The bovine coronavirus VPN has the advantages of having a good antigenic epitope and high expression in prokaryotic cells, constructing a prokaryotic expression vector, successfully establishing the BCoV indirect ELISA diagnostic method by coating the antigen with the expressed recombinant protein, determining the best conditions for the method to provide a quick and easy serological differential diagnosis method for immunological detection of bovine antibodies and epidemiological investigation, and solving current drawbacks of a diagnosis method of yak coronavirus diarrhea, which is time-consuming, laborious and cannot be diagnosed quickly, and expensive expenses of biological reagents and instruments.
Owner:SOUTHWEST UNIVERSITY FOR NATIONALITIES
Yak coronavirus detection kit based on constant temperature isolated fluorescent PCR platform and application thereof
PendingCN108085417AStrong specificityHigh sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesPositive controlFreeze-drying
The invention discloses a yak coronavirus detection kit based on a constant temperature isolated fluorescent PCR platform and an application thereof; the yak coronavirus detection kit consists of a reaction buffer liquid, a fluorescent quantitative PCR reaction liquid freeze-dried tube, and positive control and negative control freeze-dried tubes. The fluorescent quantitative PCR reaction liquid freeze-dried tube is prepared by mixed freeze-drying of a Taq enzyme, a reverse transcriptase, a primer for detection, a probe and dNTPs. The kit has the advantages of short detection time (the reaction time is only 42 minutes), high specificity, high sensitivity and easy storage (storage at the temperature of 4 DEG C), is used with cooperation of a constant temperature isolated PCR instrument, issuitable for spot rapid detection of yak coronavirus, and can be widely popularized at a basic level.
Owner:SOUTHWEST UNIVERSITY FOR NATIONALITIES
Detection agent of bovine rotavirus and bovine coronavirus, preparation and application methods of detection agent
InactiveCN102367491AQuick checkFast wayMicrobiological testing/measurementAgainst vector-borne diseasesBovine rotavirusFeces
The invention discloses a detection agent of bovine rotavirus and bovine coronavirus, which comprises two pairs of specific primers capable of amplifying a bovine rotavirus VP7 gene and a bovine coronavirus N gene. The invention also discloses a preparation method, which comprises the following steps of: selecting conserved sequences of the bovine rotavirus VP7 gene and the bovine coronavirus N gene; and designing and synthesizing the specific primers. The invention also discloses an application method which comprises the steps of: processing a sample; extracting RNA in an excrement sample; carrying out a jointed RT-PCR (Reverse Transcriptase Polymerase chain Reaction); and judging a result. Through the jointed RT-PCR, the detection agent can be used for rapidly and effectively detecting the BRV and the BCoV simultaneously so that the detection cost and the detection time are greatly reduced, has better specificity and sensitivity, and has the advantages of saving time and saving labor.
Owner:SHIHEZI UNIVERSITY
ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus
ActiveCN108318686AImproving immunogenicityStrong conservativeSsRNA viruses positive-senseVirus peptidesEscherichia coliCompanion animal
The invention discloses an ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus. The ELISA detection kit is characterized in that purified BCoV-CD isolate recombinant pET-32a-N protein is used as coating antigen, reaction conditions are optimized, and an indirect ELISA diagnosis method for detecting bovine-serum characteristic N protein antibodies. The ELISA detection kit disclosed by the invention has the beneficial effects that escherichia coli is adopted for prokaryotic expression, the raw-material sources are wide, the preparation is easy, the standardization iseasy, and the production and detection processes are safer and more reliable, so that the ELISA detection kit is suitable for being promoted and used in basic units.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Primers, probe, kit and method for detecting bovine coronavirus
InactiveCN105671209AStrong specificityImprove featuresMicrobiological testing/measurementAgainst vector-borne diseasesComplementary deoxyribonucleic acidPcr method
The invention discloses primers, a probe, a kit and a method for detecting bovine coronavirus. The primers are disclosed as SEQ ID NO:1 and SEQ ID NO:2, and the probe is disclosed as SEQ ID NO:3. The method comprises the following steps: by using cDNA (complementary deoxyribonucleic acid) obtained by carrying out RNA (ribonucleic acid) reverse transcription on the detected sample as a template, carrying out fluorescent quantitative PCR (polymerase chain reaction) amplification, and comparing the amplification curve CT value. The quantitative PCR primers are designed at the bovine coronavirus conserved gene (N gene), and a probe process is adopted to establish a fluorescent quantitative PCR method. The primers, probe, kit and method are simple and practical to operate, have the advantages of high specificity, high sensitivity and favorable repetitiveness, and are suitable for the demands for quick and accurate detection of modernized cultivation farms.
Owner:WENS FOOD GRP CO LTD
Triple RPA (recombinase polymerase amplification) detection kit for bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus
ActiveCN112853000AConvenient and quick screeningLower requirementMicrobiological testing/measurementAgainst vector-borne diseasesBovine rotavirusBovine Viral Diarrhea Viruses
The invention discloses a triple RPA (recombinase polymerase amplification) detection kit for a bovine viral diarrhea virus, a bovine coronavirus and a bovine rotavirus. The triple RPA detection kit comprises RPA detection primer groups for the bovine viral diarrhea virus, the bovine coronavirus and the bovine rotavirus, wherein the RPA detection primer groups are respectively composed of nucleotide sequences shown as SEQ ID NO.1 and SEQ ID NO.5, SEQ ID NO.8 and SEQ ID NO.13, and SEQ ID NO.15 and SEQ ID NO.18. The kit is based on a recombinase polymerase amplification method, cDNA formed by RNA reverse transcription is used as a template for amplification reaction, and a detection result is obtained by nucleic acid gel electrophoresis. The kit can be used for simultaneously detecting the bovine viral diarrhea virus, the bovine coronavirus and the bovine rotavirus, is simple to operate, has relatively high sensitivity and specificity, and provides a rapid detection reagent for on-site screening of the bovine diarrhea virus.
Owner:NORTHWEST A & F UNIV
Primer set for detecting bovine norovirus and bovine coronavirus and double RT-PCR method
ActiveCN108913815AStrong specificityIncreased sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesAgricultural scienceNucleotide
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
Triple fluorescent quantitative PCR kit for simultaneously detecting bovine rotavirus, bovine coronavirus and bovine viral diarrhea virus and application method of triple fluorescent quantitative PCR kit
InactiveCN112760421AStrong specificityImprove reaction efficiencyMicrobiological testing/measurementAgainst vector-borne diseasesBovine rotavirusBovine Viral Diarrhea Viruses
The invention provides a triple fluorescent quantitative PCR kit for simultaneously detecting a bovine rotavirus, a bovine coronavirus and a bovine viral diarrhea virus and an application, and belongs to the technical field of virus detection. The triple fluorescent quantitative PCR kit comprises three groups of specific primers and three corresponding probes aiming at the bovine rotavirus, the bovine coronavirus and the bovine viral diarrhea virus. The primer and the probe for detecting the three viruses have the advantages of strong specificity and high reaction efficiency. The triple real-time fluorescent quantitative PCR kit has the characteristics of high amplification efficiency, strong specificity, stable detection effects and good repeatability, and is suitable for dairy cow diarrhea pathogen detection, regular monitoring, dairy cow diarrhea epidemiological investigation and the like. The kit disclosed by the invention can be used for simultaneously detecting three dairy cow diarrhea pathogens in a real-time fluorescent quantitative PCR reaction system, so that the detection time is saved, the detection cost is reduced, a plurality of samples can be simultaneously detected, and the detection efficiency is high.
Owner:北京三元集团畜牧兽医总站
Bovine rotavirus fusion protein and calf diarrhea polyvalent vaccine
ActiveCN111518222AStrong immune protectionImproving immunogenicityAntibacterial agentsSsRNA viruses positive-senseBovine rotavirusEscherichia coli
The invention provides a bovine rotavirus fusion protein and a calf diarrhea polyvalent vaccine, and relates to the technical field of molecular biology. The bovine rotavirus fusion protein contains aVP6 fragment, and the VP6 fragment contains an amino acid sequence as shown in SEQ ID NO.4, wherein at least one loop region in the following (a)-(c) is replaced with an epitope derived from bovine coronavirus and / or an epitope derived from escherichia coli: (a) 168th-177th amino acid residues with an amino acid sequence as shown in SEQ ID NO.1; (b) 194th-205th amino acid residues with an amino acid sequence as shown in SEQ ID NO.2; and (c) 296th-316th amino acid residues with an amino acid sequence as shown in SEQ ID NO. 3. The bovine rotavirus fusion protein contains a plurality of epitopes, and a host can generate a plurality of antibodies after the host is immunized.
Owner:天康制药股份有限公司
Detection method for detecting bovine coronavirus
PendingCN112961944AStrong specificityHigh sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesAbsolute quantificationDiarrhea
The invention relates to a detection method for detecting bovine coronavirus based on a microdroplet type digital PCR technology. The detection method comprises a target fragment such as SEQ ID NO.1, a specific primer pair and a probe. A bovine coronavirus microdroplet type digital PCR detection kit has the sensitivity of 1copies / [mu] L, and can only be used for specifically detecting bovine coronavirus. When the kit is used for detecting clinically collected bovine diarrhea samples, the result shows that the positive rate of the kit is higher than that of existing qPCR, detection of trace viruses can be achieved, and nucleic acid can be absolutely quantified without a standard curve. The detection method can realize high-specificity, high-sensitivity and absolute quantitative detection on the bovine coronavirus, and has the advantages of high-throughput detection, higher accuracy, higher digitization, time saving and the like.
Owner:XINJIANG AGRI UNIV
Bovine rotavirus, bovine coronavirus combined inactivated vaccine and preparation method thereof
ActiveCN110124027APrevent diarrheaSeedling process optimizationSsRNA viruses positive-senseViral antigen ingredientsMaternal antibodyBovine rotavirus
The invention relates to a bovine rotavirus, a bovine coronavirus combined inactivated vaccine and a preparation method thereof. The active ingredient of the vaccine of the present invention comprisesinactivated antigen of bovine rotavirus and bovine coronavirus, and the antigenic component of the combined inactivated vaccine can be subjected to virus propagation through a continuous cell line (aiming at bovine rotavirus and bovine coronavirus) or microcarrier suspension culture (aiming at bovine rotavirus), and a seedling process and the like can be optimized. The vaccine safety, effectiveness test and antibody tracking results show that no adverse reaction is generated after immunization of cattle with the combined inactivated vaccine of the present invention, and no abortion is inducedafter immunization of pregnant cattle. Immunization of cattle produces immune protection, and newborn calves can obtain high levels of maternal antibodies through breast milk. The results show that the vaccine of the invention is safe and reliable and can be used for preventing the generation of yak diarrhea.
Owner:QILU ANIMAL HEALTH PROD
Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli
InactiveUS20050239057A1Rapid and sensitive and specificRapid and sensitive and detectionMicrobiological testing/measurementFermentationEscherichia coliBovine rotavirus
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
Attenuated bovine coronavirus and related vaccines
ActiveUS10434168B2SsRNA viruses positive-senseViral antigen ingredientsPulmonary circulation diseasesTGE VACCINE
The present invention discloses novel attenuated bovine coronavirus isolates, compositions comprising these isolate, and methods of using such compositions in vaccines, including in live vaccines. The present invention further discloses the administration of such vaccines, including the intranasal administration of such vaccines, to aid in the prevention of respiratory disease caused by bovine coronavirus.
Owner:INTERVET INC
Canine respiratory coronavirus (CRCV) spike protein, polymerase and hemagglutinin/esterase
A canine respiratory coronavirus (CRCV) that is present in the respiratory tract of dogs with canine infectious respiratory disease and which has a low level of homology to the enteric canine coronavirus, but which has a high level of homology to all bovine coronavirus strains (e.g., Quebec and LY138) and human coronavirus strain OC43.
Owner:THE ROYAL VETERINARY COLLEGE
Primer and probe combination for simultaneously detecting three infectious bovine pathogens
PendingCN114774588AImprove detection efficiencyImprove accuracyMicrobiological testing/measurementDNA/RNA fragmentationBovine rotavirusBovine Viral Diarrhea Viruses
The invention discloses a triple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer and probe combination for simultaneously detecting BVDV (Bovine Viral Diarrhea Virus), BRV (Bovine Rotavirus) and BCV (Bovine Coronaviruses), the specificity of the primer and the specificity of the probe aim at the 5 'UTR gene segment of the BVDV, the VP6 gene segment of the BRV and the N gene segment of the BCV, and the nucleotide sequences of the primer and the probe are as shown in SEQ ID No.1-9. The invention further discloses a kit for detecting the BVDV, the BRV and the BCV. According to the invention, qualitative or quantitative detection of three viruses can be realized only by performing PCR amplification on a sample once, and the method has the advantages of simplicity and convenience in operation, strong specificity, high sensitivity, good repeatability and the like.
Owner:HUAZHONG AGRI UNIV
A primer set and dual RT-PCR method for the detection of bovine norovirus and bovine coronavirus
ActiveCN108913815BStrong specificityIncreased sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesNucleotidePcr method
The invention discloses a primer set for detecting bovine norovirus and bovine coronavirus. The primer set is composed of primers for detecting bovine norovirus and primers for detecting bovine coronavirus. The core of the primer set is The nucleotide sequence is shown in SEQ ID NO.1-SEQ ID NO.4. The invention also discloses a dual RT-PCR method for detecting bovine norovirus and bovine coronavirus, the steps are as follows: (1) synthesizing the primer set for detecting bovine norovirus and bovine coronavirus; (2) extracting the primers to be tested The RNA in the sample is reverse-transcribed using the extracted RNA as a template to obtain its cDNA; (3) using the cDNA prepared in step (2) as a template, the primer set synthesized in step (1) is used for PCR amplification reaction; (4) ) to analyze PCR amplification products. The double RT-PCR detection method is simple, fast, economical and efficient, can greatly save detection time and improve detection efficiency.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
A bovine coronavirus chimeric antigen and colloidal gold immunochromatographic test paper card for detecting bovine coronavirus antibody
ActiveCN110894217BSolve the problem of rapid diagnosis of diarrheaTroubleshooting Quick Diagnosis IssuesSsRNA viruses positive-senseVirus peptidesAntigenImmunochromatographic test
The invention relates to the technical field of immune detection, and discloses a bovine coronavirus chimeric antigen and a colloidal gold immunochromatographic test paper card for detecting bovine coronavirus antibodies. The chimeric antigen sequence of the present invention is shown in SEQ ID NO:1. The present invention provides a novel bovine coronavirus chimeric antigen, which has high specificity, repeatability and accuracy when used as a bovine coronavirus antibody detection antigen, and can be applied in related immunochromatographic detection products. The results are intuitive and can be judged by the naked eye. It can quickly and specifically detect bovine coronavirus antibodies in serum, and solve the problem of rapid diagnosis of calf diarrhea in large-scale dairy farms. It has high practical value and is easy to promote and use at the grassroots level.
Owner:CHINA AGRI UNIV
A kind of bovine rotavirus, bovine coronavirus double inactivated vaccine and preparation method thereof
ActiveCN110124027BPrevent diarrheaSeedling process optimizationSsRNA viruses positive-senseViral antigen ingredientsBovine rotavirusMaternal antibody
Owner:QILU ANIMAL HEALTH PROD
A kind of bovine coronavirus ELISA detection kit
ActiveCN108318686BImproving immunogenicityStrong conservativeSsRNA viruses positive-senseVirus peptidesEscherichia coliAssay
The invention discloses an ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus. The ELISA detection kit is characterized in that purified BCoV-CD isolate recombinant pET-32a-N protein is used as coating antigen, reaction conditions are optimized, and an indirect ELISA diagnosis method for detecting bovine-serum characteristic N protein antibodies. The ELISA detection kit disclosed by the invention has the beneficial effects that escherichia coli is adopted for prokaryotic expression, the raw-material sources are wide, the preparation is easy, the standardization iseasy, and the production and detection processes are safer and more reliable, so that the ELISA detection kit is suitable for being promoted and used in basic units.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Triple rpa detection kit for bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus
ActiveCN112853000BConvenient and quick screeningLower requirementMicrobiological testing/measurementAgainst vector-borne diseasesBovine rotavirusBovine Viral Diarrhea Viruses
The invention discloses a triple RPA detection kit for bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus, comprising SEQ.ID.NO.1 and SEQ.ID.NO.5, SEQ.ID.NO. RPA of bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus composed of nucleotide sequences shown in .8 and SEQ.ID.NO.13 and SEQ.ID.NO.15 and SEQ.ID.NO.18 Detection primer set. The kit is based on the recombinase polymerase amplification method, uses cDNA formed by reverse transcription of RNA as a template to carry out amplification reaction, and uses nucleic acid gel electrophoresis to obtain detection results. The invention can be used to detect bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus simultaneously, has simple operation, high sensitivity and specificity, and provides a rapid detection reagent for on-site screening of bovine diarrhea virus.
Owner:NORTHWEST A & F UNIV
Inactivated bovine scours vaccines, process and method of preventing bovine scours
InactiveUS20050281842A1Bacterial antigen ingredientsSsRNA viruses positive-senseEscherichia coliAntigen
Inactivated scours vaccines for immunization and protection of bovine animals from disease caused by infection with bovine rotavirus and bovine coronavirus, which comprise and effective amount of at least one inactivated viral strain are described. Polyvalent inactivated vaccines further comprising an effective amount of an antigenic component which is protective against one or more additional pathogenic organisms or viruses are also disclosed. Said vaccines are prepared from one or more strains of rota- and coronavirus, C. perfringens Type C bacteria and E. coli bacteria, and combinations thereof. Preferably, a polyvalent inactivated vaccine is provided for parenteral administration. Passive immunity is achieved in neonatal calves via immunization of pregnant cows prior to birth.
Owner:ELANCO TIERGESUNDHEIT AG
Bovine coronavirus recombinant multi-epitope antigen and its application
ActiveCN107033250BAvoid infectionImprove prokaryotic expression efficiencySsRNA viruses positive-senseBacteriaMolecular ImmunologyNeutralising antibody
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI +2
Fusion protein of bovine rotavirus and multiple vaccine against calf diarrhea
ActiveCN111518222BStrong immune protectionImproving immunogenicityAntibacterial agentsSsRNA viruses positive-senseBovine rotavirusEscherichia coli
The invention provides a bovine rotavirus fusion protein and calf diarrhea multiple vaccine, and relates to the technical field of molecular biology. The bovine rotavirus fusion protein contains a VP6 fragment, and the VP6 fragment contains the amino acid sequence shown in SEQ ID NO.4, and at least one loop region in the following (a) to (c) is replaced with an antigenic expression derived from bovine coronavirus and / or antigenic epitopes derived from Escherichia coli: (a) amino acid residues 168‑177, amino acid sequence such as SEQ ID NO.1; (b) amino acid residues 194‑205, amino acid sequence such as SEQ ID NO.1 ID NO.2; (c) 296‑316th amino acid residue, the amino acid sequence is as shown in SEQ ID NO.3. The bovine rotavirus fusion protein contains multiple antigenic epitopes, and after immunizing the host, the host can produce multiple antibodies.
Owner:TIAN KANG ZHI YAO GU FEN YOU XIAN GONG SI
Detection kit for detecting bovine coronavirus (BCV) and application method thereof
PendingCN111057795AIncrease heightIncrease the speed of descentMicrobiological testing/measurementMicroorganism based processesDiseaseDisease detection
The invention discloses a detection kit for detecting BCV and a method for applying the same to non-disease detection. The detection kit for detecting the BCV comprises an amplification primer as shown in SEQ ID NO. 3 and an amplification primer as shown in SEQ ID NO. 4. The detection kit for detecting the BCV and the application method thereof can rapidly and specifically detect the BCV.
Owner:QINGHAI ACAD OF ANIMAL SCI & VETERINARY MEDICINE
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