31 results about "Bovine coronavirus" patented technology

The invention provides a bovine rotavirus fusion protein and a calf diarrhea polyvalent vaccine, and relates to the technical field of molecular biology. The bovine rotavirus fusion protein contains aVP6 fragment, and the VP6 fragment contains an amino acid sequence as shown in SEQ ID NO.4, wherein at least one loop region in the following (a)-(c) is replaced with an epitope derived from bovine coronavirus and / or an epitope derived from escherichia coli: (a) 168th-177th amino acid residues with an amino acid sequence as shown in SEQ ID NO.1; (b) 194th-205th amino acid residues with an amino acid sequence as shown in SEQ ID NO.2; and (c) 296th-316th amino acid residues with an amino acid sequence as shown in SEQ ID NO. 3. The bovine rotavirus fusion protein contains a plurality of epitopes, and a host can generate a plurality of antibodies after the host is immunized.

The present invention provides a multiplex RT-PCR / PCR method, which enables in a single assay the simultaneous detection of any combination of bovine rotavirus, bovine coronavirus, Cryptosporidium parvum, and optionally, Escherichia coli strains producing K99 pili or heat-stable enterotoxin STa.

The invention discloses a primer set for detecting bovine norovirus and bovine coronavirus. The primer set is composed of primers for detecting bovine norovirus and primers for detecting bovine coronavirus. The core of the primer set is The nucleotide sequence is shown in SEQ ID NO.1-SEQ ID NO.4. The invention also discloses a dual RT-PCR method for detecting bovine norovirus and bovine coronavirus, the steps are as follows: (1) synthesizing the primer set for detecting bovine norovirus and bovine coronavirus; (2) extracting the primers to be tested The RNA in the sample is reverse-transcribed using the extracted RNA as a template to obtain its cDNA; (3) using the cDNA prepared in step (2) as a template, the primer set synthesized in step (1) is used for PCR amplification reaction; (4) ) to analyze PCR amplification products. The double RT-PCR detection method is simple, fast, economical and efficient, can greatly save detection time and improve detection efficiency.

The invention relates to the technical field of immune detection, and discloses a bovine coronavirus chimeric antigen and a colloidal gold immunochromatographic test paper card for detecting bovine coronavirus antibodies. The chimeric antigen sequence of the present invention is shown in SEQ ID NO:1. The present invention provides a novel bovine coronavirus chimeric antigen, which has high specificity, repeatability and accuracy when used as a bovine coronavirus antibody detection antigen, and can be applied in related immunochromatographic detection products. The results are intuitive and can be judged by the naked eye. It can quickly and specifically detect bovine coronavirus antibodies in serum, and solve the problem of rapid diagnosis of calf diarrhea in large-scale dairy farms. It has high practical value and is easy to promote and use at the grassroots level.

The invention relates to a double inactivated vaccine for preventing bovine rotavirus and bovine coronavirus and a preparation method thereof. The active components of the vaccine of the present invention include inactivated antigens of bovine rotavirus and bovine coronavirus, and the antigen components of the double inactivated vaccine can be suspended by passaged cell lines (for bovine rotavirus and bovine coronavirus) or microcarriers Cultivated (for bovine rotavirus) for virus propagation, and optimized the seedling production process. The results of vaccine safety, efficacy test and antibody tracking show that there is no adverse reaction after immunizing cattle with the dual inactivated vaccine of the present invention, and no abortion is caused after immunizing pregnant cattle. All the immunized cows produced immune protection, and the newborn calves could acquire high levels of maternal antibodies through breast milk. The results show that the vaccine of the present invention is safe and reliable, and can be used to prevent the occurrence of diarrhea in calves.

The invention discloses an ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus. The ELISA detection kit is characterized in that purified BCoV-CD isolate recombinant pET-32a-N protein is used as coating antigen, reaction conditions are optimized, and an indirect ELISA diagnosis method for detecting bovine-serum characteristic N protein antibodies. The ELISA detection kit disclosed by the invention has the beneficial effects that escherichia coli is adopted for prokaryotic expression, the raw-material sources are wide, the preparation is easy, the standardization iseasy, and the production and detection processes are safer and more reliable, so that the ELISA detection kit is suitable for being promoted and used in basic units.

The invention discloses a triple RPA detection kit for bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus, comprising SEQ.ID.NO.1 and SEQ.ID.NO.5, SEQ.ID.NO. RPA of bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus composed of nucleotide sequences shown in .8 and SEQ.ID.NO.13 and SEQ.ID.NO.15 and SEQ.ID.NO.18 Detection primer set. The kit is based on the recombinase polymerase amplification method, uses cDNA formed by reverse transcription of RNA as a template to carry out amplification reaction, and uses nucleic acid gel electrophoresis to obtain detection results. The invention can be used to detect bovine viral diarrhea virus, bovine coronavirus and bovine rotavirus simultaneously, has simple operation, high sensitivity and specificity, and provides a rapid detection reagent for on-site screening of bovine diarrhea virus.

Inactivated scours vaccines for immunization and protection of bovine animals from disease caused by infection with bovine rotavirus and bovine coronavirus, which comprise and effective amount of at least one inactivated viral strain are described. Polyvalent inactivated vaccines further comprising an effective amount of an antigenic component which is protective against one or more additional pathogenic organisms or viruses are also disclosed. Said vaccines are prepared from one or more strains of rota- and coronavirus, C. perfringens Type C bacteria and E. coli bacteria, and combinations thereof. Preferably, a polyvalent inactivated vaccine is provided for parenteral administration. Passive immunity is achieved in neonatal calves via immunization of pregnant cows prior to birth.

The invention discloses a recombinant multi-epitope antigen of bovine coronavirus, also discloses application of the recombinant multi-epitope antigen of the bovine coronavirus in bovine coronavirus prevention ad diagnosis and belongs to the field of molecular immunology. An amino acid sequence of the recombinant multi-epitope antigen of the bovine coronavirus is a concatemer of a bovine coronavirus BCoV S protein epitope. The recombinant multi-epitope antigen, capable of causing a neutralizing antibody, of the bovine coronavirus serves as an immunogen or a vaccine, the neutralizing antibody oriented to the bovine coronavirus BCoV can be produced after the animal body is immunized, and the BCoV can be neutralized in vitro to prevent the animal body from virus infection. In addition, the recombinant multi-epitope antigen of the bovine coronavirus can also serve as a reagent for detecting a bovine coronavirus BCoV antibody or a polypeptide antibody.