Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus

A detection kit and coronavirus technology, applied in the direction of virus, virus peptide, virus/bacteriophage, etc., can solve the problems of long diagnosis period, high diagnosis cost, and inability to use rapid diagnosis, etc.

Active Publication Date: 2018-07-24
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Generally speaking, there are many detection methods for bovine coronavirus, including general methods such as EM, IEM, IF, IFA, HA, RT-PCR, RTFQ PCR, ELISA and many other methods, although the first few methods can accurately detect BCoV Diagnosis, but these methods have a long diagnostic cycle and high diagnostic costs, and cannot be used for rapid diagnosis of large-scale cattle farms. When a large-scale disease outbreak occurs, accurate diagnostic results and shortened diagnostic time can be better. Reduce the economic loss of the cattle farm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus
  • ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus
  • ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] A bovine coronavirus ELISA detection kit, the kit contains recombinant N protein.

[0039] The recombinant N protein is the recombinant pET-32a-N protein of BCoV-CD isolate.

[0040] The standard positive serum and negative serum of BCoV were prepared and preserved by the Infectious Disease Research Laboratory of Bayi Agricultural University, Heilongjiang. The following pMD18-T was purchased from Dalian Bao Biological Engineering Co., Ltd.; the prokaryotic expression vector pET-32a and Escherichia coli BL21 were preserved by the Infectious Disease Prevention Laboratory of Heilongjiang Bayi Agricultural University.

[0041] The recombinant N protein is a hydrophobic region with better immunogenicity predicted by DNAStar software.

[0042] The preparation method of the recombinant N protein comprises the following steps:

[0043] A primer design and synthesis

[0044] According to the isolated strain of BCoV-Mebus released on GenBank, the N gene primer sequence was des...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses an ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for bovine coronavirus. The ELISA detection kit is characterized in that purified BCoV-CD isolate recombinant pET-32a-N protein is used as coating antigen, reaction conditions are optimized, and an indirect ELISA diagnosis method for detecting bovine-serum characteristic N protein antibodies. The ELISA detection kit disclosed by the invention has the beneficial effects that escherichia coli is adopted for prokaryotic expression, the raw-material sources are wide, the preparation is easy, the standardization iseasy, and the production and detection processes are safer and more reliable, so that the ELISA detection kit is suitable for being promoted and used in basic units.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a bovine coronavirus ELISA detection kit. Background technique [0002] Diarrhea is one of the most serious diseases of newborn calves. Calves are at the greatest risk of developing diarrhea within the first month of life. The incidence of diarrhea decreases with age. Viruses, bovine coronavirus and enterotoxigenic Escherichia coli (K99) are related to four major enteric pathogens. Bovine coronavirus is the most important pathogen causing adult bovine winter dysentery (WD) and calf coronavirus diarrhea (CD). Through the investigation of antibody detection in animal serum, it was found that there is a high positive rate of bovine coronavirus in the three northeastern provinces. [0003] Generally speaking, there are many detection methods for bovine coronavirus, including general methods such as EM, IEM, IF, IFA, HA, RT-PCR, RTFQ PCR, ELISA and many other methods, althou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569C07K14/165C12N15/50C12N15/70
CPCC07K14/005C12N15/70C12N2770/20022G01N33/56983G01N2469/20
Inventor 周玉龙范春玲倪宏波朱战波侯喜林邵红武瑞任亚超高国强贾伟强王梦心胡林杰孟野盛守志王訢丞白彪慧黄晓毅
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com