The invention provides chicken
Eimeria tenella
actin depolymerizing factor (ADF) recombinant bacillus calmette-guerin and a preparation method thereof. The preparation method comprises obtaining
Eimeria tenella oocyst, extracting
ribose nucleic acid (
RNA), reversely transcribing the
RNA into complementary
deoxyribose nucleic acid (cDNA), designing primers according to an
open reading frame of a cloned
Eimeria tenella ADF
gene sequence to perform
polymerase chain reaction (PCR), enabling amplification products obtained by amplifying ADF genes to be connected with a PMD-18 vector to perform clone, performing
enzyme digestion on a
plasmid with correct sequencing identification, recycling a target fragment, enabling the
plasmid to be respectively connected with an
escherichia coli-
mycobacterium shuttle
expression vector PMV 261 and an integrated
expression vector PMV 361 which use the same
enzyme to perform
enzyme reaction, and then obtaining the positive chicken Eimeria tenella recombinant bacillus calmette-guerin through resistance screening and PCR identification. The
live vector vaccine is good in stability, easy to transport, store and produce, free of purification, capable of being directly used for
immune protection tests, removing complex processes of post-
processing of proteins and greatly reducing cost, and suitable for vast rural areas.