The invention discloses a modified new coronavirus SARS-CoV-2S protein, a recombinant plasmid and a recombinant bacillus calmette guerin vaccine constructed by the modified new coronavirus SARS-CoV-2S protein, and application of the recombinant bacillus calmette guerin vaccine, and belongs to the technical field of biological agents. Through modifying a partial sequence of gene promoter region and a partial sequence of gene terminator region, the modified new coronavirus S gene complete sequence can be smoothly expressed in an escherichia coli-mycobacterium tuberculosis shuttle plasmid pMV261 to obtain a recombinant plasmid pMVS; the recombinant plasmid pMVS is introduced into BCG through electric transformation, and recombinant BCG (rBCG) is obtained; the rBCG can successfully express S protein, arouse human immune response and induce antibody generation to prevent virus invasion, and the constructed rBCG is a subunit vaccine, has a better protection effect than parent BCG, can prevent or treat new coronavirus and mycobacterium tuberculosis, and has huge social benefits.