The invention belongs to the biomedical detection field, in particular relates to a
reagent and method used for detecting activity
tuberculosis and
latent tuberculosis infection; based on the genomic principle, the invention discloses a novel detection
reagent for
mycobacterium tuberculosis, containing
protein or polypeptide which is represented by SEQ ID1-2, 4-5, 8-28; the method uses one or a plurality of SEQ ID 1-28
protein or polypeptide to contact T cells of a
mycobacterium tuberculosis host, and detects
cytokine released from the T cells; the method can detect the tuberculosis and latent infection effectively and is not interfered by
BCG vaccine at the same time; the invention also discloses a
diagnostic reagent kit and other application based on the
protein or polypeptide and the method; compared with the T-SPOT of the prior art, the invention can improve detectable rate obviously under the condition that the specificity is not reduced; the
reagent kit has cheap price, and the cost is 1 / 5 to 1 / 10 of that of the T-SPOT reagent, thus being beneficial to being popularized in the developing countries and poor areas.