538 results about "Antibody level" patented technology

A cancer diagnostic method using a glycan array that contains Gb5 and Globo H, Bb2, Bb3, and / or Bb4.

The invention, which belongs to the technical field of vaccines, particularly relates to an African swine fever B and T cell tandem epitope fusion vaccine. The main component of the African swine fever B and T cell tandem epitope fusion vaccine is African swine fever tandem epitope fusion protein. The African swine fever tandem epitope fusion protein comprises a B cell neutralizing epitope peptidefragment and a T cell epitope; and the B cell neutralizing epitope peptide comprises the following fragments: at least one neutralizing epitope peptide of each of p72, p54, p30 proteins. When the African swine fever tandem epitope fusion protein is used as a vaccine, the immune effect is good; and the antibody level significantly higher than that of a control group can be detected after one immunization. Since the non-neutralizing epitope is reduced as much as possible in the fusion protein, the risk of accelerating virus infection (ADE effect and antibody dependence enhancement effect) by anon-neutralizing antibody after immunization can be avoided.

The invention provides biological health padding and a preparation method thereof. The biological health padding includes: rice husk, sawdust, a compound microbial agent, dandelion, argy wormwood leaves, honeysuckle, mint, cogongrass, liquorice, isatis root and water. The padding is suitable for rural small-scale farming, helps to reduce livestock diseases, is beneficial to improvement of livestock and poultry antibody levels, prevention and control of animal digestive tract, respiratory tract and other diseases, and reduction of antibiotic drug residues in animal organism of livestock and poultry, and helps to promote the sustainable development of breeding industry.

The invention discloses a novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses. The test strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially connected end to end and adhered to the bottom plate; the combination pad is coated with an SARS-CoV-2 structural protein-marker and a goat anti-rabbit IgG-marker, and the nitrocellulose membrane is provided with a detection line T1 coated with a mouse anti-human IgG monoclonal antibody, a detection line T2 coated with a mouse anti-human IgM monoclonal antibody and a quality control line C coated withrabbit IgG. When the test strip is used for quantitatively detecting SARS-CoV-2 IgG and IgM antibodies, the detection sensitivity is high, and the specificity is good and can reach 96%; the batch-to-batch difference is small, and good repeatability is achieved; the test strip can be stored for half a year at normal temperature without reducing the sensitivity and has good stability; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the levels of SARS-CoV-2 IgG and IgM antibodies in a human body, assists a nucleic acid detection means, and provides powerful support for epidemic situations.

A chimeric virus-like granular DNA vaccine able to generate stronger B cell activity, exciting high antibody level, improving cell's immunizing level by cross presentation, and activating T cell, toxic T cell and complete immunoreaction is a recombinant eucaryotic expression carrier of coding gene with chimeric protein. Its preparing process is also disclosed.

The invention provides a codon optimized porcine circovirus type 2 Cap protein coding gene and application thereof, and belongs to the field of the molecular biology. The Cap protein coding gene is as shown in SEQ ID NO: 1. The invention also provides a recombinant vector containing the gene and recombinant bacteria containing the gene. A method for preparing porcine circovirus type 2 virus-like particles comprises the steps of inducing the recombinant bacteria to express a porcine circovirus type 2 Cap protein, lysing the recombinant bacteria, and taking the lysate for purification to obtain the porcine circovirus type 2 virus-like particles. The invention also provides a gene engineering subunit vaccine with the virus-like particles as an active ingredient. The codon optimized porcine circovirus type 2 Cap is capable of realizing abundant soluble expression. The virus-like particles (VLP) are assembled from the Cap proteins and have excellent immunogenicity. A vaccine prepared from the virus-like particles provided by the invention is capable of preventing porcine circovirus type 2 infection and has the advantages of good immunogenicity, high antibody level and effective protection after challenge.

The invention discloses a panda source lactobacillus plantarum and application. The strain was preserved in China Center for Type Culture Collection on May 4, 2016 and the preservation number is CCTCC NO: M2016245. The panda source lactobacillus plantarum provided by the invention can be used for improving antibody levels of IgA, IgM and IgG immune globulins of blood serum; a condition that the percent of lymphocytes is reduced and the percent of neutrophils is increased, caused by infection and induction of enterotoxigenic escherichia coli, is inhibited; expression levels of inflammation related factors IL-1beta, IL-8, IL-6, TLR4 and MyD88mRNA (messenger Ribonucleic Acid) are remarkably down-regulated, and intestinal acute inflammation response caused by the enterotoxigenic escherichia coli is alleviated.

The present invention provides a method of detecting human antibodies in a sera solution. The invention also provides a method of quantitating anti-glycolipid antibody levels in solutions. The invention provides a method of diagnosing disease states, including neurological diseases, by quantitating a subject's antibody levels.

The invention aims to provide a Muscovy duck parvovirus. The Muscovy duck parvovirus is a YBMDV strain, and is preserved in the China General Microbiological Culture Collection Center of the China Committee Of Culture Collection For Microorganisms in the Institute of Microbiology Chinese Academy of Science at the address of No. 3, No. 1 yard, Beichen west road, Chaoyang District, Beijing, on November 11th, 2013; the preservation number is CGMCC No. 8504. The Muscovy duck parvovirus screened by the invention has the characteristics of low toxicity and high immunogenicity; dead embryo and embryo liquid can be obtained by vaccinating the Muscovy duck parvovirus to a Muscovy duck embryo; virus liquid can be collected after grinding and freeze thawing; a vaccine is prepared by ultrafiltration concentration, formalin inactivation, the addition of an adjuvant, and mixing and emulsification; the Muscovy duck parvovirus can improve antibody of the Muscovy duck, ensure parent source antibody level of an offspring, and prevent green duck parvovirus infection caused by Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention belongs to the technical field of veterinary medicaments and new biological medicaments and relates to a porcine circovirus-II vaccine potency test method. The method comprises: immunizing a white mouse with a porcine circovirus-II inactivated vaccine; and determining the PCV2 specific antibody content of the serum of the white mouse to evaluate the potency of the vaccine. When the vaccine potency test method is used, and the problems of time-consuming and labor-consuming test pig screening, large inter-batch test result difference and the like of the conventional porcine circovirus-II inactivated vaccine potency test are solved. The test method of the invention has the advantages of short test period, simple operation, low cost, accurate result and high repeatability. The method has a high application value and a promising market prospect.