The invention provides a purification method for
HBcAg (
hepatitis B
virus core
antigen)-VLP or an
HBcAg-VLP derivative. The method comprises the following steps: treating a loading solution containingHBcAg-VLP and a loading solution containing the
HBcAg-VLP derivative by hydrophobic interaction chromatographic packing, and purified HBcAg or the purified HBcAg-VLP derivative is obtained. Accordingto the purification method, the problems of numerous steps, low yield, expensive
consumables and the like of existing methods are solved, purification can be achieved by only one-step hydrophobic
chromatography; when a gel
filtration refining method is not used for treatment, the yield can reach up to 90% or higher, purity can reach up to about 86%, and the purification effect is greatly improved; if the purification method is supplemented with refining and
purification methods such as further gel
filtration, the purity can reach 99% or higher. The purification method has good application prospect and very high application value.