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Japanese encephalitis particle vaccine and preparation method and application thereof

A particle, encephalitis technology, applied in the field of immune prevention of Japanese encephalitis, to achieve the effect of protection against attack

Inactive Publication Date: 2011-07-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immunogenicity of genetically engineered subunit antigens is relatively weak and the antibody response is slow. In order to better stimulate the body's immune system and make it resistant to Japanese encephalitis virus, it is necessary to find a safe and efficient method to improve genetic engineering. Immune Effects of Subunit Vaccines

Method used

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  • Japanese encephalitis particle vaccine and preparation method and application thereof
  • Japanese encephalitis particle vaccine and preparation method and application thereof
  • Japanese encephalitis particle vaccine and preparation method and application thereof

Examples

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Embodiment 1

[0028] 1. Construction of prokaryotic expression vector PET28a-VLP-Con of hepatitis B virus core antigen skeleton protein (HBc-con)

[0029] Referring to the hepatitis B virus gene sequence (AY707087.1) in the GenBank database, the gene encoding the hepatitis B virus core antigen skeleton protein (HBc-con) was artificially designed and synthesized (its nucleotide sequence is Seq NO.3). Compared with the coding gene of hepatitis B virus core antigen 1-149Aa, the HBc-con coding gene has two artificially constructed restriction sites BamH I and EcoR I at the 231st and 238th positions, and HBc-con is at the 78th and 78th positions of HBc. Insert four amino acids into PEFS at Aa at position 79; add Nco I and Xho I sites at both ends of the hepatitis B virus core antigen skeleton protein (HBc-con) coding gene, so that the hepatitis B virus core antigen skeleton protein (HBc-con) coding gene The PET-28a vector was introduced; the hepatitis B virus core antigen skeleton protein (HBc-c...

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Abstract

The invention belongs to the field of biotechnology, and relates to a vaccine embedded with virus-like particles expressing multi-epitope antigen for Japanese encephalitis, and a preparation method and application thereof. The antigen of the vaccine is virus-like particles formed through spontaneous assembly of hepatitis B virus core antigen embedded with neutralizing antigen epitope expressing Japanese encephalitis virus and cytotoxic lymphocyte (CTL) antigen epitope, and is prepared through soluble expression of escherichia coli and purification. The Japanese encephalitis virus-like particle antigen is properly diluted with physiological saline, or is compatible with immunologic adjuvants to be prepared into the Japanese encephalitis particle vaccine. Animal experiments show that: the vaccine is safe and high-efficiency; mice inoculate with the vaccine generate high-level neutralizing antigen for the Japanese encephalitis virus to protect the mice against the attack of strong Japanese encephalitis virus by 100 percent.

Description

technical field [0001] The invention relates to a virus-like particle vaccine chimerically expressing Japanese encephalitis virus multi-epitope antigens and its preparation method and application, which belongs to the field of biotechnology and is specially used for the immune prevention of Japanese encephalitis in humans and susceptible animals. Background technique [0002] Japanese Encephalitis (JE) is a zoonotic insect-borne disease caused by Japanese Encephalitis Virus (JEV). Humans and horses show symptoms of encephalitis. Pigs mainly show reproductive disorders. Others Animals are mostly recessively infected. The disease mainly occurs in Southeast Asia. my country is one of the important epidemic areas of Japanese encephalitis. The annual number of human cases is second only to India. In recent years, with global warming and changes in the ecological environment, the distribution of Japanese encephalitis has expanded, the epidemic time has become longer, and the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/33C07K14/005C12N15/09A61K39/12A61P31/12
CPCY02A50/30
Inventor 曹瑞兵陈溥言张羽
Owner NANJING AGRICULTURAL UNIVERSITY
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