DNA vaccine
a technology of dna and vaccine, applied in the field of dna vaccine, can solve the problems of poor achievement of hypertension treatment, patients' disobedience, non-compliance with the instruction of doctors, etc., and achieve the effect of superior
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example 1
1. Method
1-1. Preparation of Construct Expressing HBc-AngII
1-1-1. Preparation of HBc-AngII, HBc Sequence and TA Cloning
[0086]Plasmid pPLc3 (Accession number LMBP 2470) was purchased from BCCM / LMBP Plasmid Collection. The following primer was designed and synthesized.
HBcF(SEQ ID NO: 23)5′-gcc atg gat atc gat cct tat aaa gaa ttc ggagc-3′HBcR(SEQ ID NO: 24)5′-ggc ctc tca cta aca ttg aga ttc ccg aga ttgaga-3′H2(SEQ ID NO: 25)5′-ggg gtg gat gta tac gcg gtc agt gat agc tgg atcttc caa gtt aac-3′H3-II(SEQ ID NO: 26)5′-c cgc gta tac atc cac ccc ttt ggt gct act agcagg gac ctg gta gtc-3′
[0087]The respective primer sequences correspond to the following positions of the nucleotide sequence (SEQ ID NO: 1) encoding the Hepatitis B virus core (HBc) antigen protein.
HBc F: the 1st-33rd nucleotides.
HBc R: the 527th-556th nucleotides.
H2: nucleotide encoding 2 amino acids (IT) of the spacer and a part of AngII sequence are linked to the 221st-241st nucleotides.
H3-II: a part of AngII sequence and 3 amino...
example 2
1. Animal Experiment
[0116]In the same manner as in Example 1, each vector was administered 3 times (100 μg / μl×2 sites / time) to the back skin of 10-week-old hypertension rat (SHR) every 2 weeks using ShimaJET (trade name, SHIMADZU CORPORATION).
HBc-AngII ISS(+) group: 6 rats
HBc ISS(+) group: 4 rats
saline group:5 rats
[0117]Administered at 0 week, 2 weeks, 4 weeks, and the blood pressure measurement, serum collection and antibody titer measurement were performed at 0 week, 4 weeks, 8 weeks, 12 weeks, 16 weeks, 20 weeks and 24 weeks from the start of the administration.
2. Blood Pressure Measurement by Tail-Cuff Method
(Method)
[0118]In the same manner as in Example 1, the blood pressure measurement was performed by a tail-cuff method. The measurement was performed 5-15 times per animal, and an average thereof was used.
(Results)
[0119]The HBc ISS(+) group (control) and saline group showed an increase in the blood pressure of SHR rats. On the other hand, HBc-AngII ISS(+) group showed a obviou...
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