43 results about "Hepatitis B Virus Antigen" patented technology

The invention provides a specific sgRNA combined with an immunogene to inhibit HBV replication, an expression vector thereof, and an application of the specific sgRNA and the expression vector. The sgRNA sequences of a human hepatitis B virogene suitable for CRISPR-Cas9 targeting editing and a PD-1 gene are designed, the plasmid vector of the sgRNA inhibiting HBV and PD-1 genes is constructed, and the plasmid vector and a nuclease gene expression vector are transferred to HBV transgenic mice, and can obviously inhibit HBV DNA replication. The gene expression vector prepared in the invention has the advantages of simple method steps, good sgRNA targeting property, and high CRISPR-Cas9 knockout efficiency. The sgRNAs specifically targeting the HBV and PD-1 genes can accurately splice the HBV and PD-1 genes, inhibit in vivo hepatitis B virus replication, and reduce the hepatitis B virus antigen expression.

Peptides are used to define epitopes that stimulate HLA-restricted cytotoxic T lymphocyte activity against hepatitis B virus antigens. The peptides are derived from regions of HBV polymerase, and are particularly useful in treating or preventing HBV infection, including methods for stimulating the immune response of chronically infected individuals to respond to HBV antigens.

A pharmaceutical composition comprising an effective amount of a compound of formulas (I-III) and a pharmaceutically acceptable carrier. Methods for treating a hepatitis virus in a patient comprising administering an effective amount of the compound of formulas (I-III) are also presented.

The invention discloses a compound of a hepatitis B virus antigen and lewis oligosaccharide and a preparation method and application thereof. The compound is prepared mainly through combination of a core hepatitis B virus antigen and lewis oligosaccharides-x. The preparation method comprises the following steps: preparing a conjugate of the core hepatitis B virus antigen and streptavidin, and combining the conjugate with (lewis oligosaccharides-x)-sodium polyacrylate-biotin through high affinity of the streptavidin and the biotin, thereby preparing the compound of the hepatitis B virus antigen and the lewis oligosaccharide. The compound enhances the capability of recognition of a immune system to the hepatitis B virus due to the active targeting of dendritic cells through the high affinity of the lewis oligosaccharides-x and DC-SIGN, can activate specific CTL responses and induce strong anti-viral immunity, and can be applied in the preparation of vaccines for treatment of the hepatitis B.

The invention provides an anti-HBV medicine combination which has more remarkable treatment effect and safety and no toxic and side effect and can evidently increase the negative conversion ratio of HBSAg. The invention takes HepG2.2.15 as a target cell and adds lamivudinevir, protocatechuic acid and the compatibility preparation of lamivudinevir and protocatechuic acid with a proper concentration to cell culturing medium. The cell is cultured by a regular method and HBsAg and HBeAg secreted by the cell is detected by ELISA method, HBV DNA content is measured by fluorescent quantitation PCR, HBV resistance effect of the drug is judged by depression rate calculation method to determine the medical treatment effect on HBV and the preparation proportion of medicine combination. The proportion of lamivudinevir to protocatechuic acid is between 1 to 50 and 1 to 100 (mass ratio) and generally the dosage of lamivudinevir is 0.05-5Mug and the protocatechuic acid is 5-500 Mug. The medicine combination can evidently improve the depression effect of the lamivudinevir on Hepatitis B antigen and reduce the toxic and side effect of the lamivudinevir when being used separately.

The invention relates to a human anti-hepatitis B virus surface antibody as well as a preparation method and application thereof. A specific hepatitis B antibody with relatively high affinity activity on hepatitis B virus antigens can be obtained by virtue of antigen-antibody binding activity screening, wherein an amino acid sequence of a light chain variable region of the antibody is shown in SEQ ID No. 1 or SEQ ID No. 2, and an amino acid sequence of a heavy chain variable region of the antibody is shown in SEQ ID No. 3 or SEQ ID No. 4. According to the human anti-hepatitis B virus surface antibody disclosed by the invention, antibody mutation hot spots are analyzed by virtue of a protein molecule modeling technology and are subjected to saturation mutation by taking an amino acid as a unit so as to construct a mutant library and perform biological verification, and thus optimization of the antibody can be achieved. The human anti-hepatitis B virus surface antibody disclosed by the invention can be used for laying a foundation for preventing hepatitis B virus infection and performing treatment research at the same time, so that the human anti-hepatitis B virus surface antibody can be applied to the prevention and treatment of liver diseases caused by hepatitis B virus infection clinically.

The invention discloses a group of thymus dependent lymphocyte antigen epitope peptide sequences of a hepatitis B virus antigen. Molecule-limited antigen peptides, such as HLA-A*0101, A*0301, A*3001, A*3101, A*3201, A*3303, and A*6801 can be specifically combined with cytotoxicity thymus dependent lymphocytes, and then the activation, proliferation and differentiation of the cytotoxicity thymus dependent lymphocytes are promoted, so as to perform the immunity effect action of resisting the hepatitis B virus. The antigen peptides can be used for preparing vaccines for treating and preventing the infection of hepatitis B virus, and also can be used for preparing detection reagents for detecting the specific cytotoxicity thymus dependent lymphocytes of the hepatitis B virus, and the potential application value is realized in the prevention, treatment and diagnosis of the hepatitis B virus.

The invention relates to preparation method for an immunological hepatitis animal model induced by hepatitis B virus whole genome transfected mouse hepatocytes, especially to an immunological inducer containing hepatocyte autoantigens and hepatitis B virus antigens and a simple operation procedure, generation of an immunological hepatitis disease model more similar to human diseases and application of the model in hepatitis pathogenesis study and anti-hepatitis drug evaluation.

This disclosure provides a multimeric hepatitis B virus (HBV) protein binding molecule, e.g., a dimeric IgA or a pentameric or hexameric IgM binding molecule, comprising at least two bivalent bindingunits, or variants or fragments thereof, each comprising at least two antibody heavy chain constant regions or fragments thereof, wherein each heavy chain constant region or fragment thereof is associated with an HBV antigen binding domain. The disclosure also provides compositions comprising the multimeric binding molecules, polynucleotides encoding the multimeric binding molecules, and methods to make and use the multimeric binding molecules.

The invention discloses application of rotundic acid in preparation of an anti-hepatitis B virus (HBV) medicine for the first time, and especially discloses application of the rotundic acid in preparation of medicines for inhibiting secretion of HBV surface antigen or HBV E antigen. In-vitro HepG22.2.15 cell pharmacological experiments prove that rotundic acid has excellent anti-HBV effect, and has the advantages of relatively low effective concentration and relatively small cytotoxicity. The rotundic acid has an effect for inhibiting HBsAg and HBeAg, has the inhibition rates for HBsAg and HBeAg respectively being 39/5 percent and 40.2 percent at 100mu g/ml, and has the inhibition effect for HBV superior to that of positive control lamivudine, oleanolic acid and ovate leaf holly bark medicinal material extract.

The invention relates to a vaccine for treating chronic hepatitis B and a preparation method and application thereof. The vaccine is prepared from a hepatitis B virus antigen and a compound adjuvant containing a molecular adjuvant. The vaccine can not only induce strong antibody response, but also induce strong cellular immune response. A carrier of the compound adjuvant used can deliver the antigen to a lymphatic system very effectively. The molecular adjuvant can activate T cell subsets and effectively increase the level and the persistence of an antibody and cellular immune response. The antibody against the hepatitis B virus antigen produced after immunization can eliminate the virus and the antigen in the blood circulation. The vaccine can quickly reduce or even eliminate the antigenof the virus in the blood, eliminate the hepatitis B virus in the liver cells, and prevent the patient from carrying the hepatitis B virus.