153 results about "Caffeoylquinic acid" patented technology

The invention involves novel drug use of six-rowed chrysanthemum plant extracts which is used to treating herpes simplex virus (type 1 and / or type 2) and various disease caused by hepatitis B virus infection. The six-rowed chrysanthemum plant extracts is prepared by six-rowed chrysanthemum plant fresh or dry goods through the refining of alcohol-water extraction, column chromatography, alcohol solvent elution, the amount of caffeoyl guinic acid chemical compound is below 30%. The six-rowed chrysanthemum plant extracts prepared in the invention has significant function of inhibiting herpes simplex virus with type 1 (HSV-1), herpes simplex virus type 2 (HSV-2) and hepatitis B virus (HBV) replication, and can reduce effectiveness of HBV e antigen (HBeAg) in the HepG 2.2.15 cell lines, it can be used for treatment various disease caused by said correlate virus infection.

The invention relates to a method for preparing an antioxidative active extractive of sweet potato leaves, and belonging to the field of agriculture products processing. The method comprises the following steps: drying and crushing fresh sweet potato leaves; performing microwave assistant extraction after the decoloring and degreasing treatment is carried out; decompressing and concentrating extract in vacuum; chromatographing through a column, and absorbing by maroporous resin; collecting eluent; decompressing and concentrating the eluent in vacuum to obtain extract of the sweet potato leaves; and atomizing and drying the extract to obtain powdered extractive of the sweet potato leaves. The product is powdered, has the unique smell of the sweet potato leaves, contains 65 to 75 percent of total phenol, and contains chlorogenic acid and 3,5-O-dicaffeoylquinic acid. The method adopts mass waste sweet potato leaves to prepare the extractive with strong antioxidative activity, turns waste into wealth, is a green industry, has simple process, no need of larger equipment investment and high extracting rate of antioxidative active substances, and is applicable to industrial production.

The invention relates to a method for simultaneously preparing total caffeoylquinic acid and stevioside by taking stevia as a raw material. The method comprises the following steps that: the stevia is extracted with water or an organic solvent under the action of heating, microwave or ultrasound-assisting, and a stevioside and caffeoylquinic acid extraction liquid is obtained after solid-liquid separation, and then is subjected to extraction separation; and finally, a pure product of total steviol glycoside and total caffeoylquinic acid is obtained after purification. On the basis of the traditional stevia production process, a total caffeoylquinic acid extraction process is added, so that the two types of products, i.e., the stevioside and the total caffeoylquinic acid, can be simultaneously obtained by taking the stevia as the raw material, the raw material source of the total caffeoylquinic acid substance is broadened, and the two types of high-value-added products can be simultaneously produced by the same raw material, and thus the production costs of the two types of products can be greatly reduced, the waste of resources and the environmental pollution can be reduced, the requirements of the sustainable development strategy can be met, and the application prospect is good.

To provide a packaged coffee beverage, which contains chlorogenic acids at high concentration, has good flavor and taste, and is suppressed in the occurrence of sediment during long-term storage. A packaged coffee beverage subjected to heat sterilization treatment, the beverage comprising (A) monocaffeoylquinic acid, (B) feruloylquinic acid and (C) dicaffeoylquinic acid, wherein (a) a total content of the ingredients (A), (B) and (C) contained in dissolved states in the beverage is from 0.14 to 4% by weight based on the beverage, and the beverage comprises (b) 80% by weight or more of water, (c) magnesium and sodium at a Mg / Na weight ratio of from 0.04 to 1, (d) a coffee extract obtained from roasted coffee beans having an L value of form 16 to 25, and (e) from 0.0024 to 0.0122% by weight of brown color in terms of Food Yellow No. 4.

The invention relates to a medicinal functional compound for health products or cosmetics, its preparing process and use thereof, wherein the compound comprises natural plants extract or monomer as the active constituents. The pharmaceutical composition can be used for treating and / or preventing diabetes and complications, cardiovascular and cerebrovascular diseases, senile dementia and hyperlipemia.

This invention relates to the new use of dicaffeoylquinic acid derivatives for treating Hepatitis B and diseases associated with retrovirus (such as HIV), the new caffeoylquinic acid derivatives and the composition containing the same.

The invention discloses a method for efficiently separating and preparing highly purified antiviral active components (single caffeoylquinic acid compound and dicaffeoylquinic acid compound) in artichoke, mainly comprising the following steps: utilizing alcohol aqueous solution to extract fresh or dry artichoke, concentrating, carrying out column chromatographic separation, preparing crude extract rich in the antiviral active components, carrying out separation and purification on the crude extract by combing high speed counter-current chromatography with high preparative performance liquid chromatography, and finally adopting a recrystallization method for refining to obtain highly purified 1-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 1,3-di-O-caffeoylquinic acid, 1,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 3,4-di-O-caffeoyl quinine acid and 4,5-di-O-caffeoylquinic acid. The method is suitable for preparing highly purified monomer by utilizing various natural products or the extractives of the natural products containing one or more components containing the above caffeoylquinic acid compounds as raw materials, wherein the natural products or the extractives are obtained from various ways; and the method has simple steps, simple operation, high efficiency, low cost and large separating preparation quantity, is easy to repeat; and the purification of the caffeoylquinic acid compounds prepared by the method can be up to 98%.

The invention relates to a method for producing dark green tea from stevia rebaudiana as a raw material. The method comprises the following steps of: selecting stevia rebaudiana tender shoots or fresh leaves or dried leaves as the raw material; after bacterial inoculation, performing pile fermentation at the temperature ranging from 15 to 35 DEG C for 1-10 d; performing roasting drying after the pile fermentation; and pressing to obtain the stevia rebaudiana dark green tea product. The stevia rebaudiana dark green tea obtained by the method provided by the invention has the effects of reducing blood pressure, resisting diabetes, resisting virus and the like as the stevia rebaudiana leaves contain bioactive substances such as stevioside, caffeoyl quinic acid, and flavone.

A caffeoylquinic acid-rich extract obtained from Erigeron multiradiatus and a method for producing the same. A caffeoylquinic acid-rich extract includes at least 15% by weight of a mixture of certain caffeoylquinic acids. The extract with the mixture of caffeoylquinic acids is highly efficacious in treating and preventing myocardial ischemia or myocardial ischemia reperfusion injuries. A method for treating or preventing a disease caused by myocardial ischemia or myocardial ischemia reperfusion includes administering a therapeutically effective amount of the caffeoylquinic acid-rich extract to a subject. Pharmaceutical compositions including the caffeoylquinic acid-rich extract are also disclosed.

The invention relates to the technical field of plant genetic engineering, and provides a gene segment DNA capable of assigning capacities to solanaceae accumulated flavonoids such as rutin, kaempferol rutin and naringenin chalcone, and caffeoyl quinic acid, wherein the gene segment has a gene sequence shown in SEQ ID NO:1, and is obtained from tomato, and comprises a control gene S1MYB12 for controlling the synthesis of solanaceae flavonoids and caffeoyl quinic acid. The inventor provides a specific method of separation cloning, function verifying and applying of the gene segment. A tomato endogenous gene S1MYB12 is driven to be specifically expressed in the tomato fruit by using a tomato fruit specificity expression promoter E8, thus the contents of the flavonoids and the caffeoylquinic acid in the tomato fruit are greatly increased, and the function of the tomato endogenous gene S1MYB12 of regulating the synthesis of the flavonoids and the caffeoyl quinic acid is verified.

The invention relates to an application of 5-caffeoylquinic acid in preparing anti-tumor drugs, belonging to the technical field of drug preparation. The invention also relates to an anti-tumor drug containing the 5-caffeoylquinic acid, which is prepared from the following components by weight percent: 0.05-99% of 5-caffeoylquinic acid and 1-99.5% of pharmaceutically acceptable carrier. The 5-caffeoylquinic acid has the effect of inhibiting angiogenesis and can inhibit the growth and transfer of tumors by inhibiting tumor angiogenesis, thus realizing the purpose of tumor resistance.

The invention belongs to the technical field of natural herbal medicines and particularly relates to an extract of a plant in blumea as well as a preparation method and application thereof. The extract contains 10-99wt% of total caffeoylquinic acid. The preparation method of the extract comprises the following steps: (S1) pulverizing the plant in blumea wholly or partially, and extracting with an organic solvent, water or the solution of the organic solvent in water to obtain a crude extract; and (S2) extracting the crude extract with n-butanol or purifying with a macroporous adsorption resin column to obtain the extract of the plant in blumea. The extract provided by the invention has a good antiviral effect, can be used for preparing medicines for preventing and treating virus infection and health care products, is definite in effective components, low in toxicity and strong in pharmacologic action and has good application prospect. In addition, the preparation process of the extract is simple and is suitable for large-scale production.

The invention discloses a separation purifying method for a DCQA (dicaffeoylquinic acid) component in jerusalem artichoke, belonging to the field of effective composition developing and utilizing. The main processing steps for extracting and separating a chemical compound in jerusalem artichoke are as follows: dried medicinal materials of jerusalem artichoke are crushed, ultrasonic or backflow extraction is carried out by ethanol (50 to 80%) in the quantity of 10 to 20 times, petroleum ether is degreased, extraction is carried out by ethyl acetate, and extraction liquid is decompressed and concentrated into an extractum. The extractum is preliminarily purified by resin or silica gel column chromatography and is further purified by SephdexLH20 so as to obtain the DCQA component with the content of higher than 85%. With simple technique and easy operation, the separation purifying method adopts diversified column chromatography to separate and purify the chemical compound of DCQA in jerusalem artichoke; the content of DCQA in a finished product is high, the source of raw materials is rich, the resin or SephdexLH20 can be recycled, and the industrialization is easy.

This invention comprises an extract of Artemisia dracunculus that can be used for the treatment and prevention of diabetes, diabetic complications, metabolic syndrome and other comorbidities that share the underlying commonality of insulin resistance. The invention includes the identity of six compounds from the extract that contribute to the activity of the extract by inhibiting protein tyrosine phosphatase-1B (PTP-1B) activity, phosphoenolpyruvate carboxykinase (PEPCK) gene expression or aldose reductase activity (ALR2). The compounds include 4,5-Di-O-caffeoylquinic acid, davidigenin, 6-demethoxycapillarisin, 2′,4-dihydroxy-4′-methoxydihydrochalcone, 2′,4′-dihydroxy-4-methoxdihydrochalcone and sakuranetin.

The invention discloses a method for preparing and partially-synthesizing chlorogenic acid from the processing residual liquid of aqua lonicerae foliae, which belongs to a method for manufacturing chemical substances or medicines and solves the problem of the existing chlorogenic acid extraction and preparation technology that a large amount of organic solvent is used and causes environmental pollution, or the production is difficult to expand as a relatively complex separation technology is used, or the extraction efficiency is low, the utilization rate of raw materials is relatively low and so on. The method comprises a deposition step, a separation step by using macroporous absorption resin, an acid hydrolysis step, a separation step by using ion exchange column, a semi-synthesis step and a crystallization step. The invention realizes comprehensive utilization of resources by adopting the processing residual liquid of aqua lonicerae foliae as a raw material, and can make full use of the caffeoylquinic acid substances during the extraction, hydrate the compounds into caffeic acid and quinic acid, then synthesize the chlorogenic acid and finally obtain chlorogenic acid with the purity over 99% through the crystallization step. The process of the invention is simple and can be easily expanded to industrial production while greatly improving the utilization rate of the raw material.

The present invention relates to a traditional Chinese medicine composition content determination method, wherein the traditional Chinese medicine composition comprises the following herbs such as forsythia suspensa, honeysuckle, radix isatidis, bitter almond, menthol, herba houttuyniae, rheum palmatum l, pogostemon cablin, dryopteris crassirhizoma, rhodiola rosea l, ephedra sinica stapf, licorice root and gypsum. According to the present invention, with the content determination method, the contents of seven components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A, 4,5-di-O-caffeoylquinic acid, quercitrin and glycyrrhizic acid are simultaneously determined, such that the energy source can be effectively saved, and the analysis cost can be reduced, wherein the traditional Chinese medicine composition content determination method of the present invention is not disclosed in the prior art.

The present invention discloses composition containing dicaffeoyl quinic acid and its preparation process. The composition is suspension, gel or solution with or without dispersing medium. The composition is easy to take, acceptable, and especially suitable for long term taking and for children, aged people and people with dysphagia. The present invention also provides the preparation process of dicaffeoyl quinic acid solution.

A method of controlling body weight in humans by administering an amount of decaffeinated green coffee extract effective to treat a subject. A preferred green coffee extract contains a ratio of 5-caffeoylquinic acid (5-CQA) and total chlorogenic acids (tCGA) (5-CQA / tCGA) of between about 0.2 and 0.3. More preferably, the concentration of tCGA is greater than about 45% and the concentration of 5-CQA is less than about 10%. A preferred method of administration consists of administering between about 200 mg and about 1,000 mg per day, more preferably administering about 400 mg per day.

The invention relates to a method for simultaneously producing honeysuckle flower distillate and various active substances. The invention relates to a method for simultaneously producing various physiologically active substances such as honeysuckle flower distillate, honeysuckle flower polysaccharide and chlorogenic acid by using honeysuckle flowers as a raw material. The method comprises the steps of vacuum distillation, precipitation separation, macroporous resin separation, and silica gel column separation. The method has the advantages of reasonable design, simple method, and easy popularization. In prior arts, a large amount of organic solvents are adopted for extracting and preparing chlorogenic acid, such that environment pollution is caused; also, the purity is not high, production cost is high, and production efficiency is low. With the method, the problems are solved, and various by-products obtained during honeysuckle flower distillate production process can be comprehensively utilized. Therefore, various physiologically active substances such as honeysuckle flower polysaccharide, high-purity chlorogenic acid, 2-caffeoylquinic acid, 3,4-caffeoylquinic acid and the like are obtained. Therefore, production cost is saved, production efficiency is improved, the process is simple, and the process can easily be applied in industrialized productions. Therefore, raw material utilization rate can be greatly improved.

The invention relates to a method for separating and purifying chlorogenic acid and 3,5-dicaffeoylquinic acid from a honeysuckle flower. According to the method, the honeysuckle flower is taken as a raw material and the method comprises the following steps: (1) preparing a honeysuckle flower crude extract; (2) extracting; (3) separating and purifying through macroporous adsorption resin; (4) separating and purifying through a semi-preparation type high efficiency liquid chromatography, namely separating and purifying by adopting the semi-preparation type high efficiency liquid chromatography to obtain two high-purity components by taking methanol-water as a mobile phase, wherein the two high-purity components are respectively identified as the chlorogenic acid and the 3,5-dicaffeoylquinic acid. The process disclosed by the invention is environment-friendly in process without severe harm on the environment and is low in integrated cost.

The present invention discloses emulsion containing dicaffeoyl quinic acid and its preparation process. The emulsion has high stability and small particle size, and may be used in intravenous injection, intramuscular injection or oral taking. The emulsion may be obtained directly via dispersing the organic solution of dicaffeoyl quinic acid and emulsifier directly into water phase component.

The invention relates to ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof. The ainsliaea fragrans champ caffeoylquinic acid extracts are prepared by the following steps of: performing refluxing extraction of the ainsliaea fragrans champ by using ethanol, filtering, concentrating and removing ethanol, adjusting the pH value to 1 to 3 by adding hydrochloric acid, applying macroporous adsorbent resin, water-washing, adding ethanol for gradient elution, and collecting eluent, of which the ethanol concentration is 50 to 70 percent; performing condensation and ethanol removal, adjusting the pH value to 1 to 3 by adding hydrochloric acid, using macroporous adsorbent resin, adding water and ethanol for gradient elution respectively, and collecting the eluent, of which the ethanol concentration is 40 to 70 percent; mixing the eluent, and vacuum drying the eluent to obtain the extracts. The UV content of the ainsliaea fragrans champ caffeoylquinic acid extracts is over 70 percent, the HPLC content is over 50 percent, and the content of 3-caffeoylquinic acid and the content of 3,5-dicaffeoylquinic acid are both over 10 percent. The ainsliaea fragrans champ caffeoylquinic acid extracts have the effects of resisting inflammations, bacteria, medicament-resistant strains, well improves pathologically changed tissues of rat suffering from cervicitis, obviously reduces the uterus index and the number of leukocytes and can be independently prepared into the medicaments for treating cervicitis or combined with other medicaments to prepare the medicaments for treating cervicitis.

A Cynara scolymus extract with a high content of caffeoylquinic acids, and a composition containing the Cynara scolymus extract with a Phaseolus vulgaris extract. The composition is useful in reducing obesity as it reduces cholesterol, triglycerides and blood glucose by sensitising the cells to insulin. This combination, when taken before meals, reduces the appetite, leading to a reduction in body weight. The extracts are preferably formulated in Oenothera biennis oil, fish oil, or oils rich in unsaturated ω-3 fatty acids.

An active oxygen eliminator derived from a natural substance, which comprises water, an organic solvent and an emulsifier and also comprises, as essential active components, p-coumarinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4-hydroxy-3-prenylcinnamic acid and 3,5,7-trihydroxy-4′-methoxyflavonol, a food comprising the active oxygen eliminator and a cosmetic comprising the active oxygen eliminator.

The invention provides the application of caffeoylquinic acid and its derivatives in the preparation of anti-complement drugs. The present invention extracts and separates the caffeoylquinic acid compounds obtained from Shamrock, through in vitro experiments, it is confirmed that the cell hemolysis caused by the activation of the classic pathway of the complement system is inhibited, indicating that there is an anti-complement effect. The activity is stronger than that of the total extract of S. sageris, and it is a kind of good complement inhibitor, which can be used to prepare new anti-complement drugs and treat various diseases caused by abnormal activation of complement, and has low effective concentration and low toxicity. The drug is safe, the source of raw materials is abundant, and it has great clinical application value. Caffeoylquinic acid and its derivatives are shown in the following structural formula I: Formula I wherein R1, R2, R3, or R4 can be the same or different, and they are H or caffeoyl respectively, provided that they cannot be H at the same time; R5 is H , CH3, CH2CH3 or CH2CH2CH2CH3.

The invention provides a novel extraction process for active ingredients in a leontopodium alpinum plant cell culture. The process adopts flavonoids, chlorogenic acid and caffeoylquinic acids as extraction indexes, integrates ultrasonic and high-temperature extraction processes, and furthest retains and extracts the total flavonoids and caffeoylquinic acids in the leontopodium alpinum plant cell culture. After verifying that the extract has antioxidant, anti-inflammatory and anti-aging activities, the extract is used for the production of a skin external preparation.

The invention discloses a multi-index component content measuring method for the roots of common peonies and honeysuckles in a Chinese herbal medicine compound preparation. The multi-index component content measuring method has the advantages that a chromatographic condition is used for measuring content of paeoniflorin and six caffeoylquinic acids in the Chinese herbal medicine compound preparation comprising the roots of the common peonies and the honeysuckles, simplicity and convenience in operation are achieved, and analysis data are accurate; gradient elution conditions are reasonable, and when both chromatographic peak shapes and separation degrees of target components are considered, detection time is shortened greatly; analysis time of analysts is saved due to simultaneous detection of the seven components, and working efficiency is improved; the proportion of organic phases in mobile phases is low, testing cost is lowered, and environmental pollution is reduced.

The invention provides a quality detection method and application of wild chrysanthemum flower formula granules. Firstly, a fingerprint spectrum of wild chrysanthemum flower formula granules is constructed; a control fingerprint is established, the similarity of different batches of wild chrysanthemum formula granules is analyzed; the quality consistency of different batches of wild chrysanthemumformula granules of different manufacturers and the same manufacturer is evaluated by means of a principal component analysis and clustering analysis mode identification method; the content of 10 chromatographic peaks in the fingerprint spectrum of the wild chrysanthemum flower formula granules is determined by a once-detection-multi-evaluation method; and on the qualitative basis, the contents ofmost chemical components in the formula granules are further defined. The detection method is accurate, feasible, simple and convenient to operate, rapid in detection and cost-saving, can be used todetermine multiple index contents of the wild chrysanthemum flower formula granules simply, conveniently and feasibly, difficulty in quality evaluation and control in different production links of thewild chrysanthemum flower formula granules can be overcome, and the method can be used to determine the contents of caffeoylquinic acid compounds and organic acids in the wild chrysanthemum flower formula granules.