Ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof
A technology of caffeoyl quinic acid and caffeoyl quinic acid, which is applied in the field of traditional Chinese medicine, can solve the problems of no cervicitis and research on the inhibitory effect of drug-resistant bacteria, and achieve good anti-infection effect, improvement of diseased tissue lesions, and avoidance of drain effect
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[0036] Preparation of reference solution
[0037] Accurately weigh the appropriate amount of 3-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, and reference substance, add 50% ethanol to make each 1 mL contain 3-caffeoylquinic acid 0.034mg / mL, 3, 5-dicaffeoylquinic acid 0.0425mg / mL mixed reference solution, set aside. Refer to the attachment for the HPLC results of the reference substance figure 2 .
[0038] According to above-mentioned chromatographic condition, sample is measured, and its HPLC result sees attached image 3 .
Embodiment 1
[0040] Take 1000g of dried medicinal material of Xingxiang rabbit ear wind, cut off (2~5cm), use 10 times of 50% ethanol, heat and reflux at 85°C to extract 3 times, filter, combine to obtain extract A, concentrate under reduced pressure at 60°C to extract A 1 / 6 times of the concentrated solution B is obtained. The concentrated solution B was adjusted to pH 2 with dilute hydrochloric acid, the acidic concentrated solution B was treated with macroporous adsorption resin AB-8, the resin was first washed with deionized water, eluted with 20% ethanol for 2 column volumes, and then washed with Elute 10 column volumes with 50% ethanol, collect the eluate with 50% ethanol concentration, concentrate under reduced pressure at 60°C to remove alcohol, and obtain concentrated solution C, which is then adjusted to pH 2 with dilute hydrochloric acid, and passed through macroporous adsorption resin For HPD-826, rinse the resin with deionized water first, then elute with water-ethanol gradien...
Embodiment 2
[0042] Xingxiang rabbit ear wind dried medicinal material 1000g, cut off (2~5cm), extracted 3 times with 15 times of 60% ethanol, heated and refluxed at 85°C, filtered, combined to obtain extract A, concentrated under reduced pressure at 60°C to extract A 1 / 7 times to obtain the concentrate B. The concentrated solution was adjusted to pH 1 with dilute hydrochloric acid B, and the acidic concentrated solution B was treated with macroporous adsorption resin HPD-826, and the resin was first washed with deionized water, eluted with 30% ethanol for 2 column volumes, and then washed with 8 column volumes were eluted with 60% ethanol, and the eluate with an ethanol concentration of 60% was collected and concentrated under reduced pressure at 60°C to obtain a concentrated solution C. 826, first wash the resin with deionized water, then sequentially elute with water-ethanol, collect eluents with an ethanol concentration of 60%, combine them, and dry them in vacuum to obtain 16g of apri...
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