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Quality detection method and application of wild chrysanthemum flower formula granules

A quality inspection method and technology of formula granules, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of high price, inconsistent standards, and inseparable from reference substances, and achieve simple operation, cost saving, and rapid detection. Effect

Active Publication Date: 2019-12-17
河南省纳普生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of wild chrysanthemum is complex, and the quality control is gradually improved. In recent years, the formula granules have been widely chosen because of their convenient portability, convenient administration, and reliable curative effect; there are many manufacturers of wild chrysanthemum formula granules, and the standards are not uniform. The product consistency of chrysanthemum formula granules, strengthening the quality evaluation and control of each link of production is a big problem
[0003] Fingerprints can fully reflect the chemical composition information of Yechrysanthemum formula granules, and have two attributes: completeness and ambiguity. The ambiguity leads to the lack of accurate qualitative and quantitative data support, which has certain limitations; content determination, especially multi-index content To a certain extent, the determination makes up for the deficiency of fingerprint method in the quality evaluation of Yechrysanthemum formula granules and the limitation of single component content determination on quality evaluation. Traditional chromatographic peak identification requires a large number of reference substances as reference, and the more chromatographic peaks are identified, the more reference substances are required. The larger the amount, the higher the research cost. The determination of the content of the external standard method is inseparable from the reference substance, and the purity of the reference substance is required. The separation and purification of the reference substance with similar structure such as caffeoylquinic acid compounds is difficult and expensive, which seriously hinders the chromatographic peak. Identification and multi-index content determination

Method used

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  • Quality detection method and application of wild chrysanthemum flower formula granules
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  • Quality detection method and application of wild chrysanthemum flower formula granules

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. Instruments and reagents

[0071] SHIMADZU LC-20AT high-performance liquid chromatography; Agilent UPLC-ESI-MS liquid chromatography mass spectrometry, KQ-500V ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); ME204 1 / 10000 electronic balance (Meite Le Toledo Instrument Shanghai Co., Ltd.); AUW 220D one-hundred-thousandth balance (Shimadzu Corporation, Japan).

[0072] Chlorogenic acid (batch number 110753-201415), caffeic acid (batch number 110885-200102), luteolin (batch number 111720-201609), mongoside (batch number 111528-201710), 3,5-dicaffeoylquinic acid ( Batch number 111782-201706), luteolin (batch number 111520-201605), apigenin (batch number 111901-201603) were purchased from China National Institutes for Food and Drug Control; 4,5-dicaffeoylquinic acid (batch number wkq16081905), 3, 4-dicaffeoylquinic acid (batch number wkq16040902), 4-caffeoylquinic acid (batch number wkq16081903), and acacetin (batch number wkq17091109) were purchased from S...

Embodiment 2

[0085]Linear range, precision drawing series mixed reference substance solution 10 μ L, with the high performance liquid chromatography condition in the embodiment 1 automatic sampling, record each chromatographic peak peak area, with peak area (Y) to injection volume (x, ng) linear Regression, 10-component linear equation, linear range, detection limit, quantitative limit are shown in Table 2.

[0086] Table 2, 10-component linear equation and linear range

[0087]

[0088]

Embodiment 3

[0090] Precision test, take 5-caffeoylquinic acid, chlorogenic acid, 4-caffeoylquinic acid, caffeic acid, 1,3-dicaffeoylquinic acid, luteolin, 3,5-dicaffeoylquinic acid An appropriate amount of quinic acid, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and mongoside were added to 50% methanol aqueous solution to prepare concentrations of 72.0 μg / mL, 207.2μg / mL, 38.5μg / mL, 3.45μg / mL, 4.24μg / mL, 8.6μg / mL, 11.5μg / mL, 15.1μg / mL, 45.9μg / mL, 28.2μg / mL mixed reference solution in Six consecutive samples were injected on the same day, and the area RSDs of 10 common chromatographic peaks were calculated. 1.15%, 1.13%, 1.06%, 0.98%, 1.21%, 1.05%, 1.24%, 0.69%; the mixed reference solution of the same concentration was injected continuously for 6 days, 3, 4, 5, 6, 7, 10, 12 , No. 13, 16, and No. 19 chromatographic peak peak area RSDs are 1.24%, 1.67%, 2.36%, 1.87%, 1.36%, 1.44%, 1.65%, 1.82%, 1.66%, 1.27%, taking No. 19 chromatographic peak as The reference peak, relative peak ...

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Abstract

The invention provides a quality detection method and application of wild chrysanthemum flower formula granules. Firstly, a fingerprint spectrum of wild chrysanthemum flower formula granules is constructed; a control fingerprint is established, the similarity of different batches of wild chrysanthemum formula granules is analyzed; the quality consistency of different batches of wild chrysanthemumformula granules of different manufacturers and the same manufacturer is evaluated by means of a principal component analysis and clustering analysis mode identification method; the content of 10 chromatographic peaks in the fingerprint spectrum of the wild chrysanthemum flower formula granules is determined by a once-detection-multi-evaluation method; and on the qualitative basis, the contents ofmost chemical components in the formula granules are further defined. The detection method is accurate, feasible, simple and convenient to operate, rapid in detection and cost-saving, can be used todetermine multiple index contents of the wild chrysanthemum flower formula granules simply, conveniently and feasibly, difficulty in quality evaluation and control in different production links of thewild chrysanthemum flower formula granules can be overcome, and the method can be used to determine the contents of caffeoylquinic acid compounds and organic acids in the wild chrysanthemum flower formula granules.

Description

technical field [0001] The invention belongs to the technical field of quality detection of medicinal materials, and in particular relates to a quality detection method and application of wild chrysanthemum formula granules. Background technique [0002] Wild chrysanthemum is the dry flower head of Chrysanthemum indicum L., a plant in the Compositae family. It has the effects of evacuating wind-heat, reducing swelling and detoxifying. The composition of wild chrysanthemum is complex, and the quality control is gradually improved. In recent years, the formula granules have been widely chosen because of their convenient portability, convenient administration, and reliable curative effect; there are many manufacturers of wild chrysanthemum formula granules, and the standards are not uniform. The product consistency of chrysanthemum formula granules, strengthening the quality evaluation and control of each link of production is a major problem. [0003] Fingerprints can fully r...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34
CPCG01N30/02G01N30/06G01N30/34
Inventor 张丽先宁二娟李飞飞曹静亚宋梦娇陈玲王韬王雪方范毅崔炜王建军
Owner 河南省纳普生物技术有限公司
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