The invention relates to an SFM (serum-
free medium) for culturing MSCs (mesenchymal stem cells). Based on volume, the SFM comprises the following components: 10.2 grams per
liter of alpha-MEM (alpha-minimum essential medium), 2.4 grams per
liter of
sodium bicarbonate, 1 to 5 millimoles of L-
glutamine, 50 to 300 milligrams per
liter of
poloxamer 188, 2 to 8 grams per liter of recombinant
human albumin, 10 to 20 milligrams per liter of recombinant human
transferrin, 2 to 10 milligrams per liter of recombinant
human insulin, 1 to 5 millimoles per liter of
Hepes, 50 nanomoles of beta-mercaptoethanol, 0.1 to 1 milligram per liter of lipid, 1 to 5 milligrams per liter of
trace element, 0.1 to 5 milligrams per liter of
glutathione, 0.5 to 5 milligrams per liter of para-
aminobenzoic acid, 1 to 50 nanograms per milliliter of
hydrocortisone, 20 to 50 milligrams per liter of
vitamin PP, 5 to 50 milligrams per liter of
vitamin C, 2 to 10mu M of compound shown in a formula I, 5 to 20mu M of compound shown in a formula II, 10 to 20 nanograms per milliliter of
progestin, 1 to 10 milligrams per liter of
putrescine, 1 to 10 international units per liter of
heparin, 1 to 10 nanograms per milliliter of EGF (
epidermal growth factor), 1 to 10 nanograms per milliliter of b-FGF (b-
fibroblast growth factor), 1 to 10 nanograms per milliliter of HGF (
hepatocyte growth factor) and 1 to 10 nanograms per milliliter of VEGF (
vascular endothelial growth factor). The SFM for culturing the MSCs is a BPS-SFM which has determinate chemical components and is free of animal-derived substances.