661results about How to "Clear ingredients" patented technology

The invention provides a preparation method for efficiently extracting separating high-purity flavone components from ginkgo leaf. The main components are kaempferol-3-O-2'', 6''-dirhamnosylglucoside, rutin, quercetin glucosyl rhamnoside, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, quercetin p-coumaroyl glucose rhamnoside and kaempferol p-oumaroyl glucose rhamnoside. The preparation method comprises the following steps: pulverizing the medicinal material ginkgo leaf, adding an alcohol water solution to carry out extraction, concentrating, carrying out nonpolar macroporous adsorbent resin column chromatography on the filtrate, carrying out two-dimensional separation with a novel silica gel substrate bonded material, eluting with a methanol solution, collecting the eluate, concentrating, and drying to obtain the gingko flavone components. The invention enhances the target component content, and obtains the flavone components with definite content. The extraction and separation process has the advantages of high repetitiveness and favorable operability, and is suitable for large-scale preparation of flavone components by separation from ginkgo leaf. The flavone components can be used as a raw material for Shuxuening injections.

The invention relates to the molecular medicine and gene treating field, in particularly, a gene segment which is new, cell target special, and can eliminate replication ability and synthesizing ability of nucleocapsid protein from source and clean the pollution protein. The gene segment is composed of a recombinant aden-associated virus vector helper plasmid non-structure and/or structure gene, and at least one copy cell special microRNA target sequence which can be used in multiple cell strains directly, and also can be used for constructing recombinant aden-associated virus plasmid and vector to prepare medicine, basic medicine and clinic gene treating.