385results about How to "Proliferation effect is good" patented technology

The invention relates to a CD47 and PD-L1 targeting bifunctional fusion protein, belongs to the field of biomedicine, and solves the problems that anti-PD-1 / PD-L1 treatment is poor in a low-immunogenicity tumor treatment effect and anti-CD47 treatment is poor in a targeting ability. The fusion protein is composed of a CD47 binding part and a PD-L1 binding part which are linked by a disulfide bond, can block the binding of CD47 and SIRPalpha, can block the binding of PD-L1 and PD-1, can achieve the effects of activating macrophages to phagocytize tumor cells and promoting antigen presentation in natural immunity, can achieve an effect of promoting the activation of tumor specific T cells in acquired immunity, and has lower blood toxicity; and as compared with the single use of anti-PD-L1 or anti-CD47 for treatment, the fusion protein has better antitumor curative effects and blood safety.

The invention relates to an industrial aquaculture fish shelter. The industrial aquaculture fish shelter is characterized in that a first fish shelter body is composed of a cover plate and a base, a gas-filling device is arranged on the cover plate, and the gas-filling device which is located in a cavity is connected with a gas bag device; the base is provided with a plurality of through holes, and a plurality of concave pits are formed in the outer surface of the cover plate; an arc-shaped panel body of a second fish shelter body is arranged on the outer side of a star-like structure and partially covers the star-like structure, and the arc-shaped panel body and the star-like structure define one or more containing bodies with the two ends opened; an anchor body of a positioning anchor is provided with a plurality of containing cavities, barbs are arranged in the containing cavities, and the barbs are in rotation connection with a convex block on the anchor body through pin shafts. According to the industrial aquaculture fish shelter, the first fish shelter body can be matched with the positioning anchor to control the suspension height of the fish shelter by inflating and deflating the gas bag, and the second fish shelter body is of a porous structure and has the advantages of being large in concealed space, high in concealing property, good in water body circulation and the like; the positioning anchor is matched with the first fish shelter body and can regulate the suspension depth to conduct artificial enhancement, and the enhancement effect is good.

In the present invention the microsphere has coated bFGF as medicine, and matrix of lactic acid-glycol copolymer (PLA-PEG). The slow-releasing bFGF microsphere is prepared by using PVA-PEG mixed liquid as dispersing medium and through evaporation and mechanical stirring. It has excellent slow-releasing performance of over two-week releasing period. Its freeze dried powder has good dispersivity and low organic solvent residue. The present invention can be used for local administration or vein administration of treating fracture, bone defect and other diseases.

The invention provides a method for promoting growth of probiotics by adding traditional Chinese medicine components into a grain culture medium. The method comprises the following steps: inoculating the probiotics into an improved MRS (Methicillin-Resistant Staphylococcus) lactobacillus culture medium or a mixed grain puffed powder culture medium added with different traditional Chinese medicine components and fermenting. Each liter of the culture medium contains 5g of mulberry leaf extract or cordyceps polysaccharide or a mixture of a plurality of traditional Chinese medicines. The probiotics are lactobacillus rhamnosus or a plurality of types of mixed probiotics. According to the method provided by the invention, the fermentation speed of the probiotics in a fermentation solution and the active bacteria number of the probiotics in the fermentation solution can be remarkably improved; the mulberry leaf extract or the cordyceps polysaccharide is added; the culture medium is fermented for 12h and the active bacteria number reaches 10<9>CFU / mL; after the grain culture medium added with the traditional Chinese medicine components is fermented, the active bacteria number reaches 4.1*10<8>CFU / mL. Meanwhile, an experiment method for detecting the active bacteria number of the probiotics in a turbid fermentation system by adopting flow cytometer detection is established.

The invention provides serum-free culture fluid for culture of salivary gland epidermal cells and salivary gland stem cells of mammals, which is prepared by using MCDB153HAA as basic culture medium and adding amino acid, vitamins, salts, lipid, trace elements, buffer fluid, hormone-like compounds, transgenic metalloprotein, antioxidants, seralbumin, glucide, purine, pyrimidine base substances and pH value indicators. The serum-free culture fluid has the advantages that salivary gland epidermal cells of the mammals can vigorously grow, good cell activity and physiological properties are maintained, in addition, the serum-free culture fluid is also suitable for the salivary gland epidermal cell culture, is particularly suitable for the field of study relevant to biological tissue engineering and belongs to the commercial cell culture fluid.

The invention discloses a preparation method of xylooligosaccharide with low DP (degree of polymerization). The preparation method is characterized by comprising steps as follows: (1) wood fiber biomass is taken as a raw material and subjected to diluted acid / diluted base / hot water pretreatment, a liquid is filtered, a solid phase is treated in a high-temperature degrading or steam explosion pretreatment manner, and the wood fiber biomass is cooled for later use; (2) xylanase is added to a pretreatment solution in step (1) for an enzymatic hydrolysis reaction; (3) an enzymatic hydrolysis solution is treated through enzyme deactivation, purification, concentration and drying, and xylooligosaccharide with low DP is prepared. The DP of finally obtained xylooligosaccharide is low by reasonably optimizing each process step, and xylobiose, xylotriose and xylotetraose are taken as main components, so that the functionality of xylooligosaccharide as a bifidus factor is enhanced. Tests verify that prepared xylooligosaccharide has a good proliferative effect on bifidobacterium animalis and lactobacillus casei. The process is simplified, the product quality is improved, and xylooligosaccharide has good application prospect.

The invention relates to probiotic preparations, and particularly relates to probiotic drops and a preparing method thereof. The probiotic drops include, by mass, 1-2.5% of unsaturated fatty acid, 85-90% of vegetable oil, 1-7% of phospholipid oil and 2-8% of a probiotic suspension. The probiotic suspension includes glycerin, Tween-80 and probiotic cells. The method includes S1) mixing and equilibrizing the probiotic cells with the glycerin and the Tween-80 according to a certain ratio, and allowing the mixture to stand for 15-30 min to achieve a system equilibrium state to obtain the probioticsuspension; and S2) fully uniformly emulsifying the probiotic suspension with the unsaturated fatty acid, the vegetable oil and the phospholipid oil according to a certain ratio to obtain the probiotic drops. The probiotic drops can improve microecology in oral cavities and noses of smoking persons and improve resistance of the smoking persons.

The invention provides a camellia chrysantha tissue culture propagation method. The camellia chrysantha tissue culture propagation method comprises the steps of obtaining aseptic seedlings, propagating the aseptic seedlings, culturing strong multiple shoots, expanding propagation and carrying out rooting culture, wherein camellia chrysantha embryos are selected as explants and then inoculated to a primary culture medium to obtain the aseptic seedlings of the camellia chrysantha; next, the aseptic seedlings are inoculated to a subculture multiplication culture medium and a strong shoot culture medium; the seedling propagation and strengthening processes are repeated and circulated until a large quantity of strong aseptic seedlings are obtained; finally, the aseptic seedlings are transplanted to a rooting culture medium for rooting culture. The camellia chrysantha tissue culture propagation method has the advantages that the vitrification problem of the aseptic seedlings in the camellia chrysantha tissue culture process is overcome, multiple subculture of materials can be realized, large-scale propagation is realized, the seedling growing period of the camellia chrysantha is shortened, the seedling growing materials are saved, the rooting rate is 95%-98%, and the transplanting survival rate is more than 90%; as a result, the camellia chrysantha tissue culture propagation method has excellent economic benefit, social benefit and ecologic benefit.

The invention relates to a chaffy dish base flavoring, and specifically relates to a nutritive chaffy dish base flavoring. The invention belongs to the field of foodstuff processing. According to the invention, spinach is added to the base flavoring. Spinach contains a large amount of beta carotene and iron, and is also an excellent source of vitamin B6, folic acid, iron and potassium. Spinach contains a large amount of anti-oxidants such as vitamin E and selenium element. Spinach has functions of aging resisting, cell proliferation promoting, brain function activating, vigor enhancing, brain aging preventing, and Alzheimer's disease preventing. Also, spinach has an antihypertensive effect, such that the base flavoring is suitable for the elderly to eat. According to the invention, xylitol is used for replacing edible sugar, such that the sugar content of the base flavoring is reduced. The base flavoring is suitable for various populations to eat, and has a nutritive function.

The present invention discloses a giant salamander active peptide and hyaluronic acid composition for effectively promoting fibroblast cell proliferation. A mass ratio of a giant salamander active peptide solution at a mass volume concentration of 1-10% and pH of 5-7 to hyaluronic acid is (1-8):1, molecular weight of the giant salamander active peptide is less than 2,000 Da, mole percentage of lysine and arginine are 7.2% and 9.7%, respectively, and molecular weight of the hyaluronic acid is 42.5-145 kDa. The giant salamander active peptide can be naturally cross-linked with the hyaluronic acid rich in multiple hydroxyl groups through hydrogen bonding, natural affinity of the hyaluronic acid and human skin cells is used, and the giant salamander active peptide and hyaluronic acid composition can effectively promote promotion of skin fibroblast proliferation and has broad application prospects in the fields of medicines and cosmetics,.

The invention discloses a method for efficiently propagating dendrobium by using roots and a culture medium of the dendrobium. The method in the invention comprises the following steps of: selecting a root section of the dendrobium, and incubating and inducing the root section to obtain an embryonic callus, wherein the embryonic callus can be greatly multiplied through subculture; transferring the embryonic callus into a protocorm induction culture medium for induction and multiplication to obtain protocorm and bud seedlings; and transferring the protocorm and the bud seedlings into an adult seedling culture medium and culturing a dendrobium rooting plant. According to the method, MS is used as a basic culture medium; 6-BA and PIC are required to be added into the culture medium for the embryonic callus induction or subculture; CPPU and NAA are required to be added into the culture medium for the protocorm induction. The method for culturing regenerated plants by using the dendrobium roots provided by the invention is high in propagation coefficient, high in planting percentage and low in cost; a seedling growing process, a seedling robusting process and a rooting progress of the protocorm can be further finished; the plants are robust, and the roots are robust.

The invention provides a method for promoting growth of probiotics by adding rutin in a culture medium. According to the method, a probiotics strain (lactobacillus rhamnosus) is inoculated into a rutin-added improved MRS lactic acid bacteria culture medium for fermentation, wherein the improved MRS lactic acid bacteria culture medium includes the following components in percentage by weight: 2% of glucose, 2% of peptone, 1% of a beef extract, 0.5% of a yeast extract, 0.5% of sodium acetate, 0.2% of dipotassium phosphate, 0.2% of diammonium hydrogen citrate, 0.025% of magnesium sulfate, 0.005% of manganese sulfate, 0.3% of tween-80, and the pH is 7.0; 1g-5g of rutin (with the impurity of 99%) is added into each liter of the fermentation culture medium. According to the method, the fermentation speed of probiotics in fermentation liquid and the viable count of probiotics can be remarkably increased; after carrying out fermentation growth on probiotics for 24 hours, the maximal viable count of obtained probiotics fermentation liquid can reach about 10<9>CFU / mL; added rutin has the effects of decreasing capillary fragility, improving microcirculation, resisting radiation and free radicals and the like, and the functional effect of a cultured product can be substantially improved.