262 results about "Raffinose" patented technology

A process for preparing a flowable slurry comprising mixing 25-70 wt. % water; an alkaline material selected from the group consisting of chlorosilicon manufacturing byproducts, direct process residue gels, cement kiln dust, and mixtures thereof; and a process aid selected from the group consisting of sucrose, raffinose, lignin, methylglucopyranoside, lactose, fructose, sodium polyphosphate, trehalose and mixtures thereof to form a flowable slurry. This slurry is especially useful in the manufacture of cement.

The invention relates to a method for deep processing cottonseeds, in particular to a method for preparing dephenolization cottonseed protein and raffinose. Gross cottonseeds are firstly delinted by a delinting machine to obtain naked seeds; after being husked by a husking machine, the naked seeds are subjected to kernel husk separation by an angle sieve to obtain cottonseed kernels and cottonseed husks; the cottonseed kernels are subjected to softening conditioning, compacting and drying; the dried blanks are sent to an extractor by a scraper blade and a closed auger and then are sprayed in stages by the No.6 solvent oil to obtain mixed oil; the mixed oil is subjected to sedimentation and centrifugal separation and purification, then is processed by a first evaporator and a second evaporator and is processed by a stripping tower to remove the solvent to obtain crude oil; the crude oil is refined to obtain edible oil; wet cotton dregs are put in a dephenolizing extractor and are subjected to liquid separation and filtration and purification to recover the solvent and also extract the crude product of the raffinose; and after being extruded, the wet cotton dregs are subjected to drying, desolventizing and conditioning and then are sent to a grinding packing department. The process technology has the advantages of high oil yielding rate, complete dephenolization, high content of active protein, simple process and low production cost.

A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering the stored biological material from storage. The invention also features a method of culturing a cell in vitro using a hypertonic medium. Carbohydrate sugars such as trehalose, sucrose, fructose, dextran, and raffinose, may be used as bio-protective agents or in the culture medium.

The invention belongs to the technical field of dairy products and relates to a preparation method of soy yogurt; the preparation method includes the following steps: preparing soy milk used for producing the soy yogurt; pretreating milk; blending the soy milk which is subject to fishy smell removal and pretreated milk; wherein, the soy milk accounts for 10-90% by weight and is added with compound stabilizer; homogenizing the blended milk materials; adding glucose, lactose, cane sugar, stachyose, raffinose or peptide fermentation promoting factors; adding a ferment starter including one strain or a plurality of grains of streptococcus thermophilus and one grain or a plurality of grains of lacbobacillus bulgaricus for fermentation; adding one or a plurality of grains of bifidobacterium adolesentis, animal bifidobacteriums or bifidobacterium lactis to prepare the soy yogurt. The method provided by the invention has the advantages of simple technique, convenient operation, short production time for fishy smell removal and fermentation, rich nutrition of the produced soy yogurt, good taste, etc.

The invention discloses a strain of yeast with strong capacities of tolerating, enriching and converting organic selenium, which is named Candida utilis CUM and has been preserved in China center for type culturecollection on April 19, 2010 with the preservation number of CCTCC M 2010090. The Candida utilis CUM has the bacterial characteristics that: cells are round, oval or sausage-shaped, and performs vegetative propagation in a mode of multi-edge germination to form pseudohypha; wort solid culture is carried out, and the bacterial colony is milk white, has smooth and moist surface, gloss or no gloss, and neat or hypha-shaped edges; the Candida utilis CUM is chemoheterotrophic, and can ferment glucose, cane sugar and raffinose; and the Candida utilis CUM is facultatively anaerobic, performs aerobic respiration under the aerobic conditions and performs alcoholic fermentation under the anaerobic conditions. The Candida utilis has strong selenium enriching capacity and good stability, can be used for producing selenious yeast, and is a strain with high research and development value.

The invention discloses base fluid for diluting semens of equus animals and a preparation method and a use method thereof. The base fluid contains A fluid and skimmed milk. The A fluid contains glucose, raffinose, D-glucopyranose, trisodium citrate, ppotassium citrate and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). The preparation method for the base fluid includes weighing various components of the A fluid into a sterile beaker, adding double-distilled water, dissolving, bringing to volume, filtering, split charging, sterilizing, and sealing to obtain the A fluid, and adding same amount of the skimmed milk into the A fluid to be evenly mixed. Users can also add penicillin and dihydrostreptomycin to the base fluid or add penicillin, dihydrostreptomycin, yolk and glycerol into the base fluid. The base fluid is suitable for semen dilution and normal temperature preservation, low temperature preservation and freezing preservation of the semens of the equus animals. The base fluid for diluting semens of equus animals and the preparation method and the use method of the base fluid have the advantages of being simple to prepare, convenient to use, good in preservation effect of the semens of the equus animals, multi-purpose, time-saving, labor-saving and applicable to mass production and application.

The invention discloses a pig feed additive which belongs to the field of feed and relates to a feed additive. The pig feed additive comprises the following components in parts by weight: 10-25 parts of yeast extract, 10-18 parts of high-temperature resistance lactic acid bacteria, 5-10 parts of soybean peptide, 8-18 parts of ganoderma fruiting body, 5-15 parts of bran, 10-22 parts of cordyceps mycelia, 1-3 parts of fructose-oligosaccharide, 1-4 parts of raffinose, 1-3 parts of cellulase, 1-2 parts of protease, 1-3 parts of mixed amino acids and 0.5-2 parts of amino acid chelated copper. After being compounded, the pig feed additive has the advantages of having very good anti-diarrhea effect, effectively enhancing the immunity of piglets, reducing the application amount of antibiotic products and improving the growth quality of the piglets; and through application of the pig feed additive, the phenomena that the piglets have long hair in a nursing stage, and the growth and development of the pigments are hindered after the nursing stage are avoided.

The invention discloses a hand cleaner composition. The hand cleaner composition comprises saccharide prebiotics, a preservative, a surfactant and water, wherein the ratio of the mass of the saccharide prebiotics to the mass of the preservative to the mass of the surfactant is (1-15):(0.1-2):(1-20); the saccharide prebiotics is at least one of polyfructosan, galacto-oligosaccharide, lactulose and raffinose; the preservative is at least one of nipagin ester, sodium nipagin acid ester, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, dehydroacetic acid, sodium dehydroacetate, benzyl alcohol and phenoxyethanol; and the surfactant is one of a nonionic surfactant, an anionic surfactant and an ampholytic surfactant. The hand cleaner composition can be used for rubbing hands without water, and has a good cleaning effect; and in addition, the saccharide prebiotics can effectively replace absorption fungus microorganisms on the surface of skin, so that the probability that bacteria directly attack the skin is eliminated.

The invention discloses a production method and an application of high-yield gamma-aminobutyric acid. The conservation number of high-yield gamma-aminobutyric acid (GABA) raffinose enterococcus M1 is CGMCC (China General Microbiological Culture Collection Center) No.5584. The production method comprises the following steps of: separating and selecting strains to obtain an Entercoccusraffinosus TCCC11660 (CGMCCNo. 5584) strain of high-yield gamma-aminobutyric acid; optimizing a fermentation culture medium and fermentation conditions; and carrying out a 10L fermentation tank experiment. The production method has the advantages of cheap raw materials, low production energy consumption, low production cost, good product safety and the like, and industrial production is easy to realize. The strain is applied to production of gamma-aminobutyric acid through glutamic acid fermentation and has good social and economic benefits.

The invention provides an oligosaccharide chelated composite trace element mineral matter supplement. The supplement is prepared through alkalizing and chelating reactions of oligosaccharides and a trace element mixture according to a molar ratio of 2.1-2.5:1; the oligosaccharide is sucrose, lactose, trehalose or raffinose; and the trace element mixture is a ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate and cobalt sulfate mixture with the molar ratio of 3-4:2-3:2-3:1:0.5-1. The oligosaccharide chelated composite trace element mineral matter supplement has the advantages of high bioavailability and small dosage as a domestic animal mineral matter supplement.

The invention discloses a technique for preparing high-purity raffinose from degreasing cotton seed meal, which takes alcoholic solution as extraction solvent to extract the degreasing cotton seed meal by percolation, and extracting solution of the raffinose is obtained; then, the extracting solution of the raffinose is absorbed by a static bed which is filled with different sorbents to separate and remove impurities; at last, the crude product of the raffinose is dissolved in the alcoholic solution for crystallization, so that white styliform raffinose crystalloid is obtained; the purity of the raffinose is over 98%, and the total yield of the raffinose is higher than 70%. The technique has short operational path, low requirements for production equipment, good quality on the obtained raffinose products, high yield and low production cost, and facilitates industrial mass production.

The invention discloses a comprehensive method for preparation of raffinose and gossypol from cottonseed meal. The method comprises the technological steps of: (1) extracting oil-extracted cottonseed meal with a polar solvent so as to obtain an extracted solution; (2) filtering the extracted solution, and removing an organic solvent in vacuum so as to obtain an aqueous phase and a solid phase; (3) subjecting the aqueous phase to filtration, flocculation, decoloration and desalination, decoloration, as well as desolventizing, thus obtaining raffinose; (4) first adding enzymes into the solid phase to carry out hydrolysis, then using an acid to adjust the solution to acidic, and performing precipitation and separation, thus obtaining a solid; (5) adding ether, halohydrocarbon, ester and / or low level ketone solvents to dissolve gossypol, and then filtering out impurities to obtain a gossypol-containing filtrate; and (6) subjecting the gossypol-containing filtrate to crystallization and crystal growing under nitrogen protection, then conducting filtration and drying, thus obtaining acicular crystals. The method can realize combined industrial production of dephenolized cottonseed protein, raffinose and gossypol, is suitable for large-scale production, and realizes comprehensive processing as well as maximum valuation of cottonseeds.

The invention belongs to the field of plant extraction, and relates to a method for extracting raffinose from cotton seed meal. The method comprises the following steps of: extracting the cotton seed meal by using water to obtain crude raffinose solution; removing macromolecular substances from the crude raffinose solution by adding a flocculating agent or using an ultrafiltration membrane to obtain primarily purified raffinose solution; adsorbing the raffinose in the primarily purified raffinose solution by using an adsorbent; eluting the raffinose from the adsorbent by using ethanol or methanol, and collecting eluent to obtain recovered raffinose solution; and concentrating the recovered raffinose solution, adding ethanol solution, adding raffinose crystal seeds for crystallizing, and separating and drying crystals to obtain purified raffinose. Compared with the conventional alcohol extraction method, the method has the advantages that the method is clean, low in energy consumption and cost, easy to operate, high in recovery rate and the like.

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

This invention relates to an isolated nucleic acid fragment encoding a galactinol synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the galactinol synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the galactinol synthase in a transformed host cell.

The invention discloses a method for extracting raffinose from cottonseed wastewater, which comprises the following steps of: performing protein removal and impurity removal pretreatment on the cottonseed wastewater, decolorizing raffinose pretreatment liquid by using polymeric aluminum and activated carbon, and crystallizing and recrystallizing in aqueous solution to obtain a raffinose crystal with the high purity of over 98 percent; and desalting residue liquid after crystallization by using macroporous resin, and performing spray drying to obtain light yellow raffinose powder with over 30 percent of raffinose, wherein the total recovery rate of two raffinose products is over 85 percent. The problems of impurity removal and decolorization of the wastewater generated by processing cottonseeds are effectively solved, high-quality raffinose is produced through crystallization by taking water as a solvent, the process is simple and has low cost, and industrial production is easy to realize.

The invention relates to a method for extracting raffinose from cottonseed meal, including following steps: removing the cottonseed rind to get cottonseed kernel, softing the embryo of the cottonseed kernel, dipping the cottonseed oil with solvent-extracted oil, getting cotton dregs; adding 40-100wt.% of carbinol in the cotton dregs, dipping for 20-60 minutes at 0-70 DEG C, after removing the carbinol, getting coarse raffinose; dissolving the coarse raffinose by water or rare acid, controlling temperature at 0-100 DEG C, pH=2-7, filtering, decoloring, concentrating, crystallize, drying, getting extractive raffinose. The invention re-exacts the raffinose after degreasing, ensures to attain the raffinose as well as perfect conttonseed oil, and reduces the dissociative gossypol content in the cotton dregs and improves the cotton dreg quality.

The present invention discloses a method for decoloring raffinose extract by adopting fixed bed type adsorptive separation. In the method, after raffinose alcohol extract is condensed and alcohol is removed, aqueous solution, the solid content of which is 10 percent, is prepared; pH is regulated to 4 to 6; the prepared solution passes through a fixed bed absorption column containing particulate active carbon, non-polar absorption resin or weak-polar absorption resin in order to remove pigments such as gossypol; and finally, 60 percent to 75 percent ethanol is used to regenerate the absorption column. In the method, the decoloring rate of the extract is more than 90 percent, and the yield of raffinose is higher than 95 percent. The method has the advantages of simple process flow, convenient adsorbent regeneration, low production cost, easy industrialized production, and the like.

The invention relates to CIK cell cryopreservation liquid. The CIK cell cryopreservation liquid is prepared from, by volume and concentration, 5-10% of DMSO, 1-2 mg / ml of grape seed extracts, 5-10 mg / ml of raffinose, 10-20% of human albumin, 40-70% of FBS and the balance PBS. Due to the fact that raffinose, grape seed extracts and human albumin are added, cell survival time is prolonged, and the cellular structure is prevented from being damaged by free radicals. By the adoption of the CIK cell cryopreservation liquid, cell viability can be better maintained, loss of cell surface antigens is reduced, and cells still have high multiplication capacity and killing activity after recovery.

A method for cryopreserving cells entails the liposomal delivery of intracellular sugar(s), such as trehalose, sucrose, raffinose, stachyose, and combinations thereof, into cells and tissues, such as red blood cells, for enhancing post-thaw viability. This method enables rapid and easy delivery of protective molecules into cells which thus greatly simplifies the preparation of cells for cryopreservation. Furthermore, as much lower concentrations of intracellular protectant are used, the method allows red blood cells containing the liposomally-delivered intracellular sugar to be transfused into a patient immediately following the thaw without having to first remove any of the cryoprotectant sugar.

A method for extracting raffinose from cottonseed meal is characterized in including the following steps: (1) extracting oil from the cottonseed meal, and extracting in 85-95 wt% methanol at the room temperature to 65 DEG C for 30-100 min. to obtain methanol extractive solution containing phenol and raffinose; (2) distilling at 80-100 DEG C for removing methanol and gossypol, and concentrating to obtain the raffinose crude product; (3) dissolving into water and / or diluted acid at the room temperature to 80 DEG C to adjust the pH value to 2-7; and (4) filtering, decoloring, concentrating, crystallizing, and drying to obtain refined raffinose. The method has the advantages of complete removal of methanol and gossypol from raffinose, high yield of raffinose, remarkable economic benefit, convenient operation, and suitability for large-scale industrialized production.

The invention provides a production method of a health instant noodle food which has a regulating effect on intestinal and gastric microecology and is characterized in adding the health instant noodle food with functional oligosaccharides which comprise xylo-oligosaccharide, galacto-oligosaccharide, manno-oligosaccharide, stachyose, raffinose, fructo-oligosaccharide, isomalto-oligosaccharide, malto-oligosaccharide, soybean oligosaccharide, chitosan, lactosucrose, gentio-oligosaccharide; the mixture comprising one or more of the oligosaccharides constitutes the functional oligosaccharides and the functional oligosaccharides of 0.02-2.00 percent by weight of flour are mixed evenly with flour and processed in the workmanship of normal instant noodle food. The functional oligosaccharides pertain to bifidus factors; therefore, the invention has the physiological function of regulating the intestinal and gastric microecology of body to relieve constipation and lowering cholesterol and blood-sugar level, thereby providing a popular health food which has the functions of preventing and curing diseases.

The present invention discloses an infant formula milk powder for improving intestinal absorption and a preparation method thereof. The infant formula milk powder comprises the following components (by weight): 10-20 parts of vegetable oil, 220-300 parts of fresh milk (containing 11.1% of dry matter), 18-30 parts of whey powder, 2-7 parts of enzymatic hydrolysis whey protein powder, 1-5 parts of raffinose, 0.15-0.2 parts of compound vitamin, 0.35-0.6 part of composite mineral, 1-1.5parts of other nutrients acceptable by the infant formula milk powder and the balance of lactose, wherein the total amount of the milk powder is 100 parts. The milk powder provided by the invention plays the role of each substance in organic combination. The infant formula milk powder is the most suitable for infant intestinal absorption and most beneficial for intestinal development from the aspects of fatty acid composition ratio, structure, quantity, and protein structure.

The invention belongs to the technical field of microorganism detection, and discloses a culture medium for detecting bifidobacterium and a detection method. The culture medium for detecting the bifidobacterium comprises a base culture medium, lactose and cysteine hydrochloride, wherein the base culture medium is prepared from a Columbia blood agar base culture medium, raffinose, lithium chloride,sodium propionate and water, and the pH (potential of hydrogen) value is 5.1. The culture medium has the advantages that besides the Columbia blood agar base culture medium, the culture medium also contains the lactose, the raffinose and the cysteine hydrochloride, and the lactose, the raffinose and the cysteine hydrochloride are used as nutritional agents to promote the growth of the bifidobacterium; when the total number of bifidobacterium is detected by the culture medium, the large colony of the bifidobacterium grows, the judging is easy, and the specificity is good; the good inhibiting function on lactobacillus helveticus is realized, and the content of bifidobacterium in the bifidobacterium and lactobacillus combined nutritional product can be accurately detected.

The invention relates to the application of a corn ZmGolS2 gene in improving the drought resistance, high temperature resistance and salt resistance capability of plants. The inventor develops a method of increasing the content of galactinol and raffinose in plant leaves by virtue of overexpression of a corn GRMZM5G872256 gene (named as ZmGoLS2) and then improving the drought resistance, high temperature resistance and salt resistance of the plants. The corn ZmGolS2 gene is cloned by use of a PCR method by virtue of homological cloning. An expression vector for regulating and controlling the gene by use of a 35S promoter is constructed. The vector is transferred into the plant Arabidopsis by use of an agrobacterium tumefaciens mediated method, and then the content of galactinol and raffinose in the transgenic plant leaves can be remarkably increased, and the drought resistance, high temperature resistance and salt resistance capability of the transgenic plants can be remarkably improved. The gene-overexpressed plants grow normally under normal conditions and are superior to the known stress-resistant gene ZmDREB2A overexpressed plants in performance. The growth of the gene is superior to the growth of the control under the stress conditions of drought, high temperatures and high salinity, and is similar to the growth of ZmDREB2A overexpressed plants.

The invention discloses a frozen sperm diluent of Boer crossbred goats. The frozen sperm diluent comprises the following components: 0-5.2g of lactose, 0-3.1g of glucose, 0-5.6g of cane sugar, 0-9.9g of raffinose, 0-2.0g of sodium citrate, 0-1.38g of citric acid, 0-0.4g of ethylenediamine tetracetic acid, 0-2.42g of trimethylol amino-methane, 100ml of distilled water, 15-20ml of yolk, and one hundred thousand IU of benzylpenicillin potassium. After adopting the frozen sperm diluent of Boer crossbred goats, the unfrozen sperm is high in energy and low in price; the problem of long-term storage of semen can be solved; the semen is not restricted by the time, region and breeding life; interprovincial and international exchange is facilitated; the utilization rate of excellent male animal is improved to the maximal extent; and the breeding and improving paces of the variety are accelerated. Meanwhile, excellent breeding stock is subjected to descendant measurement in a short period; excellent hereditary characters of a variety or an individual body are preserved and restored; and significances are achieved in the aspects such as bloodline updating, introduction, breed conservation and reduction of production cost rise.

The present invention discloses a crystallization method for preparing high-purity raffinose. C1 to C3 alcohol aqueous solution is adopted as crystallizing solvent, raffinose in the form of small crystal particles is used as inoculating seeds to induce crystallization, and after two to twenty four hours of grain growing, the high-purity raffinose is obtained by filtration and drying. The purity of the obtained raffinose product can reach over 99 percent, and the crystallization yield can reach over 80 percent. The method has the advantages of simple technique, low production cost and easy industrialized production.

The invention discloses a composite immunity reinforcing agent for cyprinus carpio var. koi. The reinforcing agent comprises the following components in percentage by mass: 35 to 45 percent of raffinose, 25 to 35 percent of low molecular chitosan oligosaccharide, 15 to 20 percent of glycyrrhetic acid and 10 to 15 percent of lactic acid bacillus. The reinforcing agent can obviously reinforce the immunity and the disease resistance of the cyprinus carpio var. koi and improve the survival rate by 12 to 19 percent; the immune effect of the composite immunity reinforcing agent is remarkably higher than that of a single immunity reinforcing agent, and the composite immunity reinforcing agent also can overcome immune suppression caused by long-term use of a single product; and a method for preparing the composite immunity reinforcing agent has the advantages of stable and low-cost raw material source and simple production process.