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Base fluid for diluting semens of equus animals and preparation method and use method thereof

A technology of base liquid and diluent, applied in the preservation, application, and animal husbandry of human or animal bodies, which can solve the problems of low conception rate and short storage time of sperm motility, so as to ensure the survival rate and be suitable for large-scale production and application, the effect of prolonging the survival time

Inactive Publication Date: 2012-07-18
QINGDAO DERUI JUNFA BIOLOGY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of the traditional semen diluents such as short storage time of sperm motility, low conception rate and ready-to-use and ready-to-use, the present invention provides a base liquid for dilution of semen of equine animals and its preparation method and use method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weigh glucose 24g, raffinose 1.6g, D-lactose monohydrate 1.6g, trisodium citrate Na 3 C 6 h 5 o 7 2H 2 Put O 0.24g, potassium citrate 0.4g and HEPES 4.7g into a sterile beaker, add double distilled water to dissolve, constant volume, filter, sub-package, sterilize and seal to obtain liquid A; Bacteria and sealed liquid A marked the preparation time, stored at 0-5°C, and taken as needed; take an appropriate amount of liquid A and add an equal volume of skim milk to mix evenly to obtain the base liquid of Example 1.

[0036] Add penicillin in an amount of 1000 units / ml and dihydrostreptomycin in an amount of 1000 ug / ml to the base liquid and mix well to obtain the dilution liquid of Example 1.

[0037] Comparative test 1

[0038] The traditional diluent glucose-yolk dilution (control group 1) and milk dilution (control group 2), and the diluent of Example 1 (example group 1) were used to dilute horse semen, respectively. Among them, after placing the diluted solutio...

Embodiment 2

[0044] Weigh 23g of glucose, 1.7g of raffinose, 1.5g of D-lactose monohydrate, trisodium citrate Na 3 C 6 h 5 o 7 ·5H 2 Put O 0.27g, potassium citrate 0.35g and HEPES 4.6g into a sterile beaker, add double distilled water to dissolve, constant volume, filter, sub-package, sterilize and seal to obtain liquid A; Bacteria and sealed liquid A marked the preparation time, stored at 0-5°C, and taken as needed; take an appropriate amount of liquid A, add an equal volume of skim milk and mix well to obtain the base liquid of Example 2.

[0045] Add penicillin and dihydrostreptomycin in an amount of 1000 units / ml and dihydrostreptomycin in an amount of 1000 ug / ml respectively to the base solution and mix well to obtain the dilution solution of Example 2.

[0046] Comparative test 2

[0047] The traditional diluent glucose-yolk dilution (control group 3) and glucose-methyl sodium tartrate-egg yolk dilution (control group 4) and the diluent of Example 2 (embodiment group 2) were use...

Embodiment 3

[0053] Weigh 25g of glucose, 1.5g of raffinose, 1.7g of D-lactose monohydrate, Na 3 C 6 h 5 o 7 ·5H 2 Put O 0.3g, potassium citrate 0.45g and HEPES 4.8g into a sterile beaker, add double distilled water to dissolve, constant volume, filter, sub-package, sterilize and seal to get A liquid; Bacteria and sealed liquid A marked the preparation time, stored at 0-5°C, and taken as needed; take an appropriate amount of liquid A and add an equal volume of skim milk to mix evenly to obtain the base liquid of Example 3.

[0054] Add penicillin at 1000 units / ml, dihydrostreptomycin at 1000ug / ml, egg yolk at 2.5-3.5% and glycerin at 2.5-3.5% to part of the base solution, mix well, The dilution of Example 3 was obtained.

[0055] Comparative test 3

[0056] The traditional diluent lactose-egg yolk-glycerin dilution (control group 5) and lactose-EDTA-sodium citrate-egg yolk-glycerin dilution (control group 6), and the dilution solution of Example 3 (embodiment group 3) Dilute donkey ...

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Abstract

The invention discloses base fluid for diluting semens of equus animals and a preparation method and a use method thereof. The base fluid contains A fluid and skimmed milk. The A fluid contains glucose, raffinose, D-glucopyranose, trisodium citrate, ppotassium citrate and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). The preparation method for the base fluid includes weighing various components of the A fluid into a sterile beaker, adding double-distilled water, dissolving, bringing to volume, filtering, split charging, sterilizing, and sealing to obtain the A fluid, and adding same amount of the skimmed milk into the A fluid to be evenly mixed. Users can also add penicillin and dihydrostreptomycin to the base fluid or add penicillin, dihydrostreptomycin, yolk and glycerol into the base fluid. The base fluid is suitable for semen dilution and normal temperature preservation, low temperature preservation and freezing preservation of the semens of the equus animals. The base fluid for diluting semens of equus animals and the preparation method and the use method of the base fluid have the advantages of being simple to prepare, convenient to use, good in preservation effect of the semens of the equus animals, multi-purpose, time-saving, labor-saving and applicable to mass production and application.

Description

[0001] technical field [0002] The invention relates to the technical field of animal semen preservation, in particular to an equine animal semen dilution base solution and a preparation method and use method thereof. [0003] Background technique [0004] Semen dilution is to add a diluted protection solution suitable for sperm to survive in vitro and maintain sperm vitality in the semen. Fully improve the utilization rate of semen and the breeding efficiency of good male animals. Semen dilution should be carried out as soon as possible after collection. Undiluted semen is not conducive to the survival of sperm, especially when the temperature of semen is low (below 20°C), sperm are subject to low temperature shock and are prone to cold shock. Only diluted sperm are suitable for storage, transportation and insemination, so semen dilution is an important technical link in artificial insemination of animals. [0005] At present, the breeding of Equus animals in my country...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 荣美洁邹志刚张志鹏
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
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