The invention relates to a manual simulation plant climate box, which comprises a box body and an equipment room, wherein the equipment room is located at the back side of the box body; the box body is constituted of a top cover, a base plate and a side plate; an electrical box, a refrigerating unit, a humidifier and a carbon dioxide steel cylinder are set in the equipment room; the box body is also provided with a camera, an ultraviolet ray lamp tube, a sensor unit, a plant cultivation frame and an air processor; an air flow channel is set between a top plate and the top cover and the air flow channel is shaped like a long strip; the top plate is provided with an inserted buckle; an inserted-type light-supplementing lamp bar is mounted in the inserted buckle; and a top plate at the outskirt of the inserted-type light-supplementing lamp bar is provided with a ventilation hole. The advantages are that comprehensive simulation to natural environment or specified environment is performed; each of factor is controlled separately and also coordinated as a whole; specified light strength, temperature, humidity and carbon dioxide concentration can be acquired; the best growth environment of plants in each stage inside the box body can be simulated; and comparative test can be performed by controlling certain environmental factors.