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Composite immunity reinforcing agent for cyprinus carpio var. koi

An immune enhancer, koi technology, applied in the fields of application, animal feed, additional food elements, etc., can solve the problems that there are no research reports on koi compound immune enhancer, and achieve immune suppression, stable source, and production process simple effect

Inactive Publication Date: 2011-04-06
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there is a single immune enhancer at present, there is no research report on the compound immune enhancer for improving the disease resistance of koi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Weigh 40 kg of raffinose, 30 kg of low-molecular-weight chitosan, 18 kg of glycyrrhizic acid and 12 kg of Bacillus subtilis, grind them into powder respectively, pass through a 200-mesh sieve, and then mix them uniformly in a mixer to make the variation of the obtained mixture Coefficient ≤ 5%, that is the complex immune enhancer for koi of the present invention. The koi were cultured in the indoor recirculating culture system of four experimental sites in different regions of the country. The initial weights of the fish were: 5.72±0.36 g, 12.32±0.78 g, 21.36±1.15 g and 35.78±2.18 g. No compound immune enhancer for koi of the present invention was added to the feed used in the control group, and 0.1% (mass percentage) of the product of the present invention was added to the feed used in the experimental group. There were 3 replicates in the control group and the experimental group respectively. After 56 days of feeding experiments, the survival rates of koi fed with th...

Embodiment 2

[0013] Weigh 35 kg of raffinose, 35 kg of low molecular weight chitosan oligosaccharide, 20 kg of glycyrrhizic acid and 10 kg of Bacillus subtilis, grind them into powder respectively, pass through a 200-mesh sieve, and then mix them uniformly in a mixer to make the variation of the obtained mixture Coefficient ≤ 5%, that is the complex immune enhancer for koi of the present invention. Carry out the field breeding test in two places in different regions of the country respectively, the breeding experiments of two fishes and the experiments of the control group are all carried out in 3 ponds respectively, and the average area of ​​each pond is 5 mu, and the feed of the control group does not add the present invention Koi with compound immune booster. Add 0.2% product of the present invention in the experimental feed. After a 60-day feeding experiment, the survival rate of the fish increased by more than 12% compared with the control group. Moreover, the three main immune indi...

Embodiment 3

[0015] Weigh 45 kg of raffinose, 25 kg of low molecular weight chitosan oligosaccharide, 15 kg of glycyrrhizic acid and 15 kg of Bacillus subtilis, grind them into fine powder respectively, pass through a 200 mesh sieve, and then mix them uniformly in a mixer to make the obtained mixture Coefficient of variation ≤ 5%, that is the complex immune enhancer for koi of the present invention. Carry out koi field breeding test in different areas, obtain the result similar to embodiment 1.

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PUM

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Abstract

The invention discloses a composite immunity reinforcing agent for cyprinus carpio var. koi. The reinforcing agent comprises the following components in percentage by mass: 35 to 45 percent of raffinose, 25 to 35 percent of low molecular chitosan oligosaccharide, 15 to 20 percent of glycyrrhetic acid and 10 to 15 percent of lactic acid bacillus. The reinforcing agent can obviously reinforce the immunity and the disease resistance of the cyprinus carpio var. koi and improve the survival rate by 12 to 19 percent; the immune effect of the composite immunity reinforcing agent is remarkably higher than that of a single immunity reinforcing agent, and the composite immunity reinforcing agent also can overcome immune suppression caused by long-term use of a single product; and a method for preparing the composite immunity reinforcing agent has the advantages of stable and low-cost raw material source and simple production process.

Description

technical field [0001] The invention relates to a feed additive for fish, in particular to a compound immune enhancer for koi. Background technique [0002] With the development of recreational fishery, koi ( Cyprinus carpio var.koi ) has become one of the more popular and popular large-scale ornamental fish, and its breeding scale is constantly expanding and its intensification degree is constantly improving. At present, my country has become the main producer and exporter of koi in the world, and koi breeding has made important contributions to the optimization and adjustment of my country's fishery industry structure and the increase of export earnings. With the change of koi breeding mode, the requirements for artificial feed preparation technology are getting higher and higher, while traditional breeding mainly focuses on artificial feed preparation technology for body color improvement, which can no longer meet the needs of sustainable development of modern koi aquacu...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16A23K10/16A23K10/30A23K20/163A23K50/80
Inventor 林仕梅罗莉潘瑜
Owner SOUTHWEST UNIVERSITY
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