2359 results about "CHITOSAN OLIGOSACCHARIDE" patented technology

The invention relates to a chitosan-based transport system for overcoming the blood-brain barrier. This transport system can convey active agents or markers into the brain. The transport system contains at least one substance selected from the group consisting of chitin, chitosan, chitosan oligosaccharides, glucosamine, and derivatives thereof, and optionally one or more active agents and / or one or more markers and / or one or more ligands.

The invention relates to a plant stress-resistant inducer, which takes Chitosan oligosaccharide solution as an effective active component. The invention adopts Chitosan oligosaccharide to induce crops to resist drought, waterlog, cold and freeze. Leaves of the crops are sprayed with the Chitosan oligosaccharide or roots of the crops are irrigated with the Chitosan oligosaccharide in order to induce the stress resistance (drought, waterlog, cold and freeze resistance) of the crops. By inducing the leaves of the crops, the content of resistant enzyme is increased in different degrees; the praline content is obviously increased; the opening degree of stomata obviously declines; the chlorophyll content is obviously increased; and the stress resistance of the crops is improved. The external application of the Chitosan oligosaccharide can improve the stress resistance of the crops, is free from toxicity, causes no environmental pollution, and is simple in usage.

The invention relates to a plant wound protective agent containing chitosan oligosaccharide which contains chitosan oligosaccharide and one or more of a nutritional agent, an adjusting agent and a sterilizing agent, wherein the weight percentage of the chitosan oligosaccharide accounting for the protective agent is 0.0015-0.03 percent. The plant wound protective agent can be used for spraying or smearing. The prepared solid preparation for smearing also contains a film-forming agent. On one to five days before pruning, girdling or grafting, the wound is sprayed by using the protective agent; and on one to five days after the pruning, girdling or grafting, the wound is smeared by the protective agent, so that disease attacks of the wound can be reduced and the healing of the wound can be promoted. The plant wound protective agent is used for apple trees, pear trees, peach trees, jujube trees, grape trees, mango trees, longan trees, lychee trees, citrus trees, watermelon, maidenhair trees, honeysuckle, Arabian jasmine flower and Chinese rose.

The present invention discloses one kind of pesticide composition containing oligosaccharide in effective amount and carrier. The present invention also discloses its preparation process and use in resisting plant diseases. The composition of the present invention can stimulate the defense reaction of rice and other plants to strengthen disease resistance.

The invention discloses a preparation method of biocompatible gamma-polyglutamic-acid hydrogel, which comprises the following steps of: by using polyglutamic acid as a main raw material and a biological-source material chitosan oligosaccharide as a cross-linking agent, carrying out preparation reaction in an aqueous solution, and activating a gamma-polyglutamic-acid carboxyl group by utilizing activating agents diamine carbide and N-hydroxysuccinimide ester to enable the polyglutamic acid and the free amino group of the chitosan oligosaccharide to form an amido bond to further form the hydrogel in a three-dimensional netted structure. After the prepared gel is swelled and balanced, drying is carried out by adopting a freeze-drying, vacuum-drying or microwave-drying mode. The biological-source material chitosan oligosaccharide is selected as the cross-linking agent, so that the biocompatibility of an obtained hydrogel system can be markedly improved; and a reaction system is an aqueous-solution system, reaction regulating and controlling properties are good, and the gel is stable. Because the hydrogel prepared by the method disclosed by the invention has good biocompatibility, the hydrogel can be widely applied to medicament carriers, auxiliary wound materials, medical and sanitary articles and the like.

The invention belongs to the field of plant protection, and in particular relates to an agricultural composition taking oligosaccharide and sulphur as synergistic components and an application of the agricultural composition in prevention and treatment of agricultural pests. The mass part ratio of oligosaccharide to sulphur is 1:(0.1-200) and the mass part ratio of oligosaccharide to sulphur to other pesticide components is 1:(0.1-200):(0.1-100). In the composition, oligosaccharide is pectin oligosaccharide, sodium alginate oligosaccharide or chitosan oligosaccharide. The synergistic composition containing oligosaccharide and sulphur can be processed to particles, suspending agents and the like with other insecticidal and mite-killing components or sterilizing components to realize the synergistic effect of the agricultural composition, and the agricultural composition is used for preventing and treating nematode, red spiders, Phyllocoptruta oleivora Ashmead or powdery mildew of crops.

The present invention relates to a method for preparing chitin and chitosan oligosac charide. The method comprises the steps that usual raw materials such as the crust of shrimp and crab, the insect crust or the fungal mycelia, etc. are micronized through the dry process or wet process; the carapace material of the obtained fine powder raw material is decalcified with the chemical process, and then is defatted and deproteinized with the method of micro-organism compound enzyme coarse enzyme liquid co-enzymolysis, and the insect and fungus fine powder thereof is directly defatted and deproteinized; a whole cell immobilizing bioreactor of a chitin deacetylase high-yield producing strain is prepared, to perform the circulatory deacetylation to the chitin and then obtain chitosan with corresponding degree of deacetylatoion; obligate anaerobic acid-producing bacterium and high-yield producing chitosan bacterium are utilized, the chitosan is submerged and fermented in the liquid, to obtain chitosan oligosaccharide with high water solubility. The present invention has the advantages that the method is helpful to fully utilize the resources, and makes the waste to the worth, at the same time, the default of the manufacturing process of the chemical process can be avoided, the production efficiency is improved, the energy is saved, the consumption is reduced, the byproduct with corresponding high value added can be produced, the comprehensive economic benefits of the relative secondary industry are obviously improved, the industrial development is promoted, and the multi-win effect is attained.

The invention relates to an alga microorganism fertilizer agent and a preparation method thereof and belongs to the technical field of aquiculture and the like. The alga microorganism fertilizer agent is fermented in such a way that monosodium glutamate waste liquid, alga paste, photosynthetic bacteria, actinomycetes, azotobacters and bacillus subtilis are compounded, and meanwhile, chitosan, chitosan oligosaccharide, microelements and molasses are added according to appropriate parts. The alga microorganism fertilizer agent is formed in such a way that the monosodium glutamate waste liquid is adopted for fermentation, and the raw materials preparation is simple; high-content compound bacteria are contained (the content of the bacillus subtilis is larger than three hundred million / mL, and the total content of the photosynthetic bacteria, the actinomycetes and the azotobacters is larger than one hundred million / mL) and the fertilizing effect is obvious; and the alga microorganism fertilizer agent also has a function of purifying water body, has a wide application range, and is suitable for sea-water and fresh-water cultivation.

Disclosed is a surfacely modified hydrophobic Chitosan oligosaccharide polymer drug-loaded colloidal cluster, obtained by grafting chitosan with a average molecular weight of 1.5kD-51kD and aliphatic acid of C10-C22, comprising based on hydrophobic modified Chitosan oligosaccharide polimer colloidal clusters, modifying amidogens or hydroxy groups of Chitosan oligosaccharide molecules on polimer surface with bi-functional small organic moleculers, forming chemical bond bridges between molecules on the surfaces of polymer colloidal clusters,to improve the instability of polymer colloidal cluster diluted, and to imorove the original loose structure of polimer colloidal cluster surfece to form a fine and close net-shaped structure, to decrease a sudden release of drugs, and to control drugs with slow release. The drug-loaded colloidal cluster provided is a nano-carrier with excellent organelle targeting, is applicated in life science field and pharmacy field.

The invention discloses an oligosaccharide-containing foliar fertilizer composite which is characterized in that: the composite comprises oligosaccharide with effective treatment quantity and foliar fertilizer with various elements, wherein the content of the oligosaccharide is 1%-5% of the total weight of the composite; the oligosaccharide comprises chitosan oligosaccharide, sodium alginate oligosaccharide and pectin oligosaccharide; the content of the element foliar fertilizer is 5%-50% of the total weight of the composite; and the element foliar fertilizer comprises at least one of nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, boron, ferrum, manganese, copper, zinc and molybdenum. The composite is applied to field crops, commercial crops and fruit and vegetable crops. The oligosaccharide which can induce plants to resist diseases and promote a root system to grow is introduced into the foliar fertilizer, so that the foliar fertilizer composite can promote the growthand the nutrient absorption of plant root systems, and has a certain control effect for restraining the happening of the diseases.

A ‘bioactive’ composition that has one or more oligogalacturonans ((1→4)-α-D-galacturonan) or any other oligosaccharides (oligoguluronans) that may present an ‘egg box’ conformation, this conformation being further stabilized by one or more polycationic saccharide(s), preferably either a chitosan oligosaccharide or a chitosan polysaccharide. A method prepares this composition and it is used, in medical, pharmaceutical, agricultural, nutraceutical, food, feed, textile, cosmetic, industrial and / or environmental applications.

A composition of a second-class mixture comprising chitosan oligosaccharide and trace elements (or chelates thereof) or additional phytohormone, and the preparation technology thereof are provided.

The invention discloses a bactericide pesticide composition containing chitosan oligosaccharide. The bactericide pesticide composition comprises a component A and a component B, wherein the component A is chitosan oligosaccharide or oligosaccharide derivatives; and the component B is one or more of active compounds. The bactericide pesticide composition has a synergistic effect, overcomes or delays pesticide resistance of bactericides, improves stress resistance and yield of crops, adjusts the metabolic balance in plants, improves the quality of agricultural products and enhances the application security of bactericides, and is safer for an ecological environment and people; and the using amount of chemical synthetic bactericides is reduced.

The invention discloses a chitosan oligosaccharide composite with cold-resistant function, which is aqueous solution, wherein the mass concentration of the chitosan oligosaccharide is 5%-10%, other auxiliary active constituents include at least one of phosphorus, potassium, calcium, boron, molybdenum, zinc, salicylic acid and betaine, and the content of the auxiliary active constituents is 0.5%-30% of the total mass concentration of the composite. Before the flowering period and the young fruit period of fruit trees, the time of young seedling of melon and fruit, vegetables and flowers, and the cold sensitive period of the winter growth of greenhouse crops, the composite is diluted by 1000 times with water to be sprayed on the leaves of the plants for 2-3 times; the chitosan oligosaccharide composite used in the invention has the function of promoting photosynthesis of the crops, improving activation performance of various enzymes and promoting metabolism of carbohydrates; and the chitosan oligosaccharide composite is used for inducing the crops to improve stress resistance and inducing the crops to improve cold resistance, so that the crops can get through the low-temperature cold harmful period, and regular development and yield of the crops are ensured.

The invention relates to a pesticide composition for relieving the phytotoxicity of herbicide. Effective components are a plant growth regulating agent and active polysaccharide, wherein the plant growth regulating agent is any one or a mixed agent of two of compound nitrophenolate and diethylaminoethyl hexanoate; the active polysaccharide is any one or a mixed agent of two of chitosan oligosaccharide and poly-glucosene; and the pesticide composition comprises the effective components in parts by weight of 0.05-90.0 parts of the plant growth regulating agent and 0.05-90.0 parts of the active polysaccharide. The pesticide composition comprehensively utilizes the synergism effect of the plant growth regulating agent and the active polysaccharide to resist disease source force and relieve the phytotoxicity of the herbicide. The pesticide composition is further applicable to crop exogenous damages including hormone phytotoxicity, low-temperature frozen injury, salt alkaline injury and the like, and also can be used for crop diseases of crops virus diseases, verticillium wilts and the like.

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

The invention discloses a special fertilizer for fruit trees and a preparation method thereof. The fertilizer comprises a base fertilizer and a leaf fertilizer, wherein the base fertilizer is prepared from the following raw materials: an organic fertilizer, urea, monoammonium phosphate and potassium chloride, the organic fertilizer is prepared by fermenting raw materials, namely dry chicken manure, cornstalk, rice husk, fruit tree leaves, bean cakes and needle mushroom dregs by virtue of complex microbial inoculants, and the leaf fertilizer comprises the following components: urea, monopotassium phosphate, farm-oriented chitosan oligosaccharide, 6-BA, multi-effect triazole, triazolone, polyethylene glycol 200, sodium tetraborate and microelements. The fermented organic fertilizer not only contains a large quantity of beneficial bacterium, organic matters and multiple microelements, but also can be used for increasing the beneficial bacterium of the soil, fix nitrogen, dissolve phosphorus, activating the soil after being applied, improving the water retentivity, fertilizer using efficiency and fertilizer persistent effect of the soil, and achieving the effect of a controlled-release fertilizer; in addition, the leaf fertilizer is applied in different growing stages of the fruit trees, thus supplementing nitrogen, phosphorus, potassium and microelements, promoting to bear and expand fruits, and improving the stress resistance of the fruit trees.

The invention discloses a beverage capable of maintaining beauty and nourishing the skin and a preparing method of the beverage. The beverage capable of maintaining beauty and nourishing the skin is prepared from collagen tripeptide, chitosan oligosaccharide, gamma-aminobutyric acid, fish collagen peptide and skipjack elastin peptide. The beverage achieves great effects of adjusting and repairingthe skin of the human body in combination with multiple collagen nutrients, vitamin C and nutrient substances resistant to sugar and oxidation required for the human body.

The invention belongs to the field of plant growth regulators and application thereof, and in particular relates to a composition containing chitosan oligosaccharide and a plant growth regulator and application of the composition to promotion of growth and yield of plants. The plant growth regulator is composed of one or more of forchlorfenuron, diethyl aminoethyl hexanoate, compound sodium nitrophenolate, naphthylacetic acid, heteroauxin, polyamine, cytokinin and indolebutyric acid, wherein the weight ratio of the chitosan oligosaccharide to the plant growth regulator is 1:(0.01-50), and the application dosage of the composition is 0.05-10 ppm. The composition is applied to any plant required by humans directly or indirectly.

The invention discloses a high-efficiency multifunctional foliage fertilizer and a production method thereof. The foliage fertilizer is prepared by chelating shrimp processing leftovers, which serve as the main raw material, with medium and minor elements. The foliage fertilizer is prepared from 10-30% of amino acids, 2-8% of medium and minor elements, 10-30% of organic nitrogen, 3-20% of phosphorus, 3-20% of potassium, and 10-30% of shrimp peptide protein, chitosan, chitosan oligosaccharide, chitin, glucosamine hydrochloride, astacin and shrimp cephalin. The foliage fertilizer disclosed by the invention enhances the low-temperature tolerance, drought tolerance, salt damage tolerance, chemical damage tolerance and other stress tolerances of crops on the premise of comprehensively and efficiently supplementing necessary nutrients for crops, and has the functions of restoring the wound in the root system, enhancing the disease resistance of the crops, inhibiting disease and pest and the like. The production technique can reduce the acidolysis consumption, avoids introducing abundant inorganic salts and chloride ions to destroy soil ecological equilibrium and influence plant growth, lowers the energy consumption and requirements for equipment strength, and can implement the clean production of complete utilization and zero pollution discharge.

The invention discloses a chitosan oligosaccharide capsule; chitosan oligosaccharide with 2000-10000 of molecular weight, radix salviae miltiorrhizae extract, pueraria root extract, obtuseleaf senna seed extract and schisandra extract are used as main materials; starch and magnesium stearate are taken as auxiliary raw materials; the weight components of the formula of the chitosan oligosaccharide capsule include the chitosan oligosaccharide, the radix salviae miltiorrhizae extract, the pueraria root extract, obtuseleaf senna seed extract, schisandra extract, the starch and the magnesium stearate; the production process of the capsule is that: sieving, weighting, mixing, capsule filling, polishing, screening, inside and outside package, warehouse-in inspection are carried out to prepare the health-care product. The chitosan oligosaccharide capsule has the advantages that the main composition of the capsule comprises the chitosan oligosaccharide, general flavone and total anthraquinone; the capsule is taken orally two times one day, 3 granules each time, and the capsule has auxiliary protection function to the chemical liver damage.

The invention discloses a multifunctional nutrient ecological dense agent, and preparation and application methods thereof. A formula of the multifunctional nutrient ecological dense agent comprises a solution (A) and a solution (B), wherein the solution (A) is an EM compound probiotic fermentation solution; the solution (B) consists of an organic nutrition adsorbed and complexed chelation carrier and a combined inducer; the EM compound probiotic fermentation solution is prepared by fermentation, activation and expanding propagation of an EM solid bacterial agent; the organic nutrition adsorbed and complexed chelation carrier is a concentrated molasses fermentation solution; active components of the combined inducer are selected from a group consisting of photosynthetic induction factors, tetramethyl glutaric acid, RE nitrate acid rare earth for agricultural use, activator protein, chitosan oligosaccharide, salicylic acid, S-abscisic acid, trehalose, a rooting agent, uniconazole and forchlorfenuron; the solution (A) and the solution (B) are separately stored, and are mixed for preparation before being used. The multifunctional nutrient ecological dense agent is applicable to horticultural crops in greenhouses and large lands, and can be used with ecological cultivation soil in a cooperating manner for high-quality ecological cultivation; by virtue of various application modes such as seed soaking, dripping and spraying, multiple targets of supplying organic nutrition, regulating and inducing resistance to disease and pest, and performing adversity protection are achieved.

The invention discloses a compound composition used for increasing the crop fertilization rate. The compound composition comprises a first component of phethalanilic acid, a second component selected from one or more of brassinolide, 6-benayl aminopurine, compound sodium nitrophenolate, diethyl aminoethyl hexanoate, prohexadione calcium, grain yellowing agents, forchlorfenuron, triacontanol, chlormequat chloride, mepiquat chloride, paclobutrazol, uniconazole, amino-oligosaccharin, humic acid, amino acid, alginic acid and chitosan oligosaccharide, and a third component selected from one or more of boric fertilizer, calcium fertilizer, nitrogen fertilizer, potash fertilizer, phosphatic fertilizer, iron fertilizer, copper fertilizer, magnesium fertilizer, manganese fertilizer, molybdenum fertilizer and silicon fertilizer. The composition can be prepared into an aqueous solution or a soluble solution or wettable powder or water dispersible granules which are allowed to be used for agriculture. The compatibility of all the components is reasonable, the key risks of the phethalanilic acid are lowered while it is ensured that the crop fertilization rate is effectively increased, the nutrition level and stress-resistant capacity of crops are improved, and the increase of production and income of crops is promoted.

The invention provides chitosan oligosaccharide laying hen feed which is prepared from laying hen feed and a premix material in parts by weight. The laying hen feed is prepared from the following components in parts by weight: 62-64 parts of corn, 16-18 parts of bean pulp, 6-8 parts of rape seed dregs, 1-2 parts of fish meal, 1-2 parts of calcium bicarbonate, 3-4 parts of shell powder, 4-6 parts of talc powder, 0.4-0.6 part of soybean oil, 0.5-0.6 part of salt, 3-4 parts of wheat bran, 4-5 parts of sorghum flour and 3-4 parts of blood powder; and the premix material is prepared from the following components in parts by weight: 4-6 parts of laying hen vitamin, 3-5 parts of Vc, 20-22 parts of choline chloride, 1-2 parts of antioxidizing amine, 60-70 parts of protein powder and 1-2 parts of oligosaccharins. The chitosan oligosaccharide laying hen feed provided by the invention has the advantages of simple process and convenience in manufacture and use, and the oligosaccharins which is rich in chitosan oligosaccharide is added into the feed for feeding the laying hen so that the nutrition component of the obtained egg products contains the chitosan oligosaccharide.

The invention discloses an insecticide acaricide taking an oligose and macrolides composition as a synergist, and in particular relates to a synergism composition capable of enhancing the insecticiding effect of the insecticide acaricide. The composition comprises oligose and a macrolides insecticide; the macrolides insecticide is abamectin, pleocidin, emamectin benzoate or ivermectin; the oligose is pectic oligosaccharide, sodium alginate oligosaccharides or chitosan oligosaccharide; the mass part ratio of the oligose to the macrolides insecticide is (1-30):(1-10). The synergism composition can enhance the insecticidal efficacy of the insecticide acaricide and realize the synergism effect with other insecticide acaricides.

The invention discloses a compound feed for preventing fatty liver of tilapia and a preparation method of the feed. The compound feed is prepared from the following raw materials according to a certain proportion: fish meal, fermented soybean meal, meat and bone meal, rapeseed meal, cottonseed meal, wheat bran, wheat flour, calcium dihydrogen phosphate, multi-vitamin premix, multi-mineral premix, soybean oil, soybean lecithin, chitosan oligosaccharide, astragalus polysaccharide and choline chloride. The preparation comprises the following steps of: respectively crushing and screening all the raw materials (except the soybean oil) by a crushing machine, weighing the flour, the calcium dihydrogen phosphate, the multi-vitamin premix, the multi-mineral premix, the soybean lecithin, the chitosan oligosaccharide, the astragalus polysaccharide, the choline chloride and other raw materials according to the mass fraction in the formula and mixing uniformly, further weighing the remaining raw materials, uniformly stirring and mixing, fully uniformly mixing with water, performing extrusion and pelletization by a feed machine, and drying at the temperature of 55-60 DEG C to prepare the feed for the tilapia. The compound feed has balanced nutrition, is suitable for requirements of healthy growth of the tilapia and can further reduce the accumulation of fat in livers of the tilapia and improve disease resistance of the tilapia.

The invention relates to an agricultural composition for nematode control and its application. The composition comprises a nematicide and a polysaccharose substance. Specifically, the nematicide can be one or several of lythidathion, isazofos, ivermectin, profenofos, and thiodicarb. The polysaccharose substance can be chitosan or chitosan oligosaccharide. The composition of the invention combinesthe indoor activity generated by the mixing of a nematicide and a polysaccharose substance on nematodes, and reaches an unexpected synergistic effect. The polysaccharose substance can improve the physiochemical performances of soil so as to inhibit nematode activity, and can enhance the stress resistance of crops, thus having diseases prevention and growth promotion effects. Adding of the nematicide into the polysaccharose substance improves the nematode killing effect of the nematicide and simultaneously promotes crop growth.

The invention discloses a fruit color enhancer and a method for promoting fruit coloration by using the same. The fruit color enhancer comprises photosynthetic bacteria, and also can comprise at least one of anthocyanin solution, chitin, chitosan, chitosan oligosaccharide, potassium dihydrogen phosphate, potassium sulfate and a wetting agent. The fruit color enhancer is sprayed on the surfaces of fruits or leaves for 1 to 5 times 1 to 45 days before the fruits are harvested, and the effect is more obvious when the fruit color enhancer is directly sprayed on the fruits instead of the surfaces of the leaves. By the method, harmful substances are not remained in the fruits, and sugar degree and hardness are improved to a certain extent. The method is suitable for the fruits of apples, grapes, cherries, tomatoes and the like, and has great significance for improving appearance and internal quality, and increasing the value and export yield of the fruits, and the income of peasants.

The invention discloses essence containing chitosan oligosaccharide and jellyfish collagen and a facial mask manufactured from the essence. The essence is prepared by mixing the following components: chitosan oligosaccharide, jellyfish collagen, lecithin, hydroxyl prolinol ester silanol, hydroxyethyl ethylcellulose, xanthan gum, a portulaca oleracea extract, glycerol, butanediol, phenoxyethanol, ethylhexylglycerin and water. The facial mask can be manufactured by soaking a carrier facial mask, that is, a biological cellulose facial mask containing chitin, into the essence till being completed wetted. The essence of the facial mask contains chitosan oligosaccharide and jellyfish collagen, and has a good synergistic effect with the carrier facial mask (the biological cellulose facial mask containing chitin). Compared with an ordinary cellulose facial mask (such as cellulose obtained from wood pulp and cellulose obtained from coconuts), the biological cellulose facial mask containing chitin is relatively low in density, relatively large in pore surface, high in aperture, relatively strong in absorption property and capable of conducting more active matters.

The invention relates to plant wound liquid containing chitosan oligosaccharide, which is characterized by comprising a plant growth regulator, wherein the regulator is one or more of naphthalene acetic acid and humic acid and accounts for 1 to 50 percent of the total weight of the liquid; and the chitosan oligosaccharide accounts for 0.0015 weight percent to 0.03 weight percent of the total weight of the liquid. The liquid can be prepared into any liquid preparation for spraying. One to five days before plants are trimmed, grafted or girdled, the liquid is used for spraying a wound. The liquid is used for apple trees, pear trees, peach trees, jujube trees, grape vines, mango trees, longan trees, lychee trees, mandarin trees, watermelons, gingko trees, lonicera japonica, jasmine, Chinese rose and the like.