Method for preparing chitin and its chitosan and chitosan oligosaccharide

Abstract

The present invention relates to a method for preparing chitin and chitosan oligosac charide. The method comprises the steps that usual raw materials such as the crust of shrimp and crab, the insect crust or the fungal mycelia, etc. are micronized through the dry process or wet process; the carapace material of the obtained fine powder raw material is decalcified with the chemical process, and then is defatted and deproteinized with the method of micro-organism compound enzyme coarse enzyme liquid co-enzymolysis, and the insect and fungus fine powder thereof is directly defatted and deproteinized; a whole cell immobilizing bioreactor of a chitin deacetylase high-yield producing strain is prepared, to perform the circulatory deacetylation to the chitin and then obtain chitosan with corresponding degree of deacetylatoion; obligate anaerobic acid-producing bacterium and high-yield producing chitosan bacterium are utilized, the chitosan is submerged and fermented in the liquid, to obtain chitosan oligosaccharide with high water solubility. The present invention has the advantages that the method is helpful to fully utilize the resources, and makes the waste to the worth, at the same time, the default of the manufacturing process of the chemical process can be avoided, the production efficiency is improved, the energy is saved, the consumption is reduced, the byproduct with corresponding high value added can be produced, the comprehensive economic benefits of the relative secondary industry are obviously improved, the industrial development is promoted, and the multi-win effect is attained.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to a new process for producing chemical products and new materials, in particular to a method for preparing chitin, chitosan and chitosan oligosaccharide from various raw materials. Background technique [0002] As we all know, chitin (chitin, chitin) is one of the most widely existing natural organic compounds on the earth, ranking second only to cellulose in quantity, with an annual biosynthesis amount of nearly 10 billion tons; chitin is also The largest amount of nitrogen-containing natural organic compounds on the earth except protein; these two points alone are enough to explain the important position of chitin in nature and human life. Studies in recent decades have shown that chitin and its deacetylated product chitosan, as well as various derivatives of the two, as new chemical materials and organic products, have extremely wide application value in people's production and life , in...

AI Technical Summary

Problems solved by technology

[0003] Although chitin is recognized as an excellent natural organic raw material of extraordinary value, it is not easy to extract from its original material as a tissue structure component of various animals, microorganisms and some plants

In recent decades, the mainstream process for extracting chitin / chitosan and preparing its derivatives at the actual production level is the chemical method. At present, it has been found that there are a series of disadvantages as follows: 1) Excessive use of strong acids, strong Alkali and harmful chemical reagents produce a large amount of waste water and waste, which seriously pollutes the environment, especially the water environment; 2) The high-frequency, long-term high-temperature treatment process produces huge energy consumption; 3) The preparation process requires corrosion-resistant and high-temperature-resistant equipment , increase production costs; 4) non-specific catalytic action of chemical catalysts, so that different chemical structure components can not be clearly and effectively dissociated and / or synthesized, affecting the yield and purity of the target product; 5) strong acid, strong base and other extraction The severe chemical reaction conditions of the solvent obviously restrict the stability and quality control of the target chemical composition; 6) The frequent washing and purification procedures in the process and the difficulty in the recovery and treatment of dilute acid and dilute alkali lead to a large amount of precious clean fresh water being consumed and wasted ; 7) The chemical characteristics of strong acid, strong alkali and other extraction solvents such as corrosiveness and explosiveness pose a safety threat to the working environment and human health

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