The invention discloses a method for high-flux identification of
ralstonia solanacearum resistance. The method comprises the following steps: sterilizing
quartz sand with particle size
ranging from 2 mm to 4 mm at high temperature of 121 DEG C for 30 minutes, cooling, and spreading into an aseptic 12-pore
cell culture plate, wherein each pore is filled with 10 g of the
quartz sand and 4 ml of aseptic water;
sowing flue-cured tobacco seeds to be tested onto the
quartz sand of the 12-pore
cell culture plate, after
sowing, and culturing by the 12-pore
cell culture plate under the conditions of 25 DEG C, RH of more than 80% and alternative 16-hour lighting and 8-hour shading; after the
flue-cured tobacco seeds germinate, adding a Hoagland's
nutrient solution into the 12-pore
cell culture plate for culturing the
flue-cured tobacco seeds under the aseptic condition, continuously culturing under the conditions of 25 DEG C, RH of more than 80% and alternative 16-hour lighting and 8-hour shading, and identifying the
disease resistance of the flue-cured tobacco seeds each with 5-6 leaves, wherein each pore is filled with 2 ml of the Hoagland's
nutrient solution; preparing inoculating liquid and inoculating; investigating and grading the
disease states. According to the method, the
ralstonia solanacearum resistance can be identified with
high flux and high efficiency and a large amount of flue-cured tobacco varieties can be identified in one time.