139 results about "Gibberella" patented technology

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

A molecular marker associated with the major QTL of wheat resistance to head blight is disclosed. The SSR labelling technique is used to analyze the 894037 / Alondra F4 recombinant selfing population. The existance of major QTL on the chromosome 3B of Ning 894037 is detected, and this can be used to explain 42.8% of head blight resistance. The obtained molecular markers Xgwm 533-130, BARC 133-115 and BARC 075-110 for the major QTL of Ning 894037 can be used to culture the disease-resistant wheat variety with high efficiency.

The invention discloses a gibberella and a method for fermentation production of a gibberellin GA4+7. The method belongs to the field of fermentation engineering. The method comprises the following steps of plate culture, seed culture and fermentation culture, wherein in the fermentation culture, condition control is carried out. A mixed carbon source is added into a fermentation culture medium; and at a particular moment, the fermentation culture medium with the mixed carbon source is added with vegetable oil and a characteristic precursor and an oxygen flux of the fermentation culture medium with the mixed carbon source is increased so that a GA4+7 valence is improved. The gibberellin GA4+7 obtained by gibberella fermentation has a high valence and a low cost, is convenient for operation and is conducive to industrial production.

The invention discloses a panax notoginseng antimicrobial peptide gene PnSN1. The nucleotide sequence of the PnSN1 gene is shown as SEQ ID NO:1, and the PnSN1 gene is used for coding snakin antimicrobial peptide. The functional genomics correlation technique study proves that the PnSN1 gene has the function of improving the plant fungal infection resistance capability; the antifungal PnSN1 gene is built onto a plant expression vector and is transferred into tobaccos for overexpression; the transgenosis tobacco has high in vitro antifungal activity. The transgenosis tobacco subjected to PnSN1 overexpression has the strong inhibiting effect on the growth of fusarium oxysporum, fusarium solani, verticillium fusarium, botryosphaeria dothidea and beaded gibberella.

The invention belongs to the field of genetic engineering, and discloses a receptor protein kinase gene and an expression vector and application thereof. The complementary deoxyribonucleic acid (DNA) sequence of the receptor protein kinase gene TaRLK-B is a sequence identifier number 1 (SEQ ID N0.1), and the coded amino acid sequence of the receptor protein kinase gene TaRLK-B is an SEQ ID NO.2. The gene is from a wanghuibai variety of common wheat (Triticum asetivumL.), and is reported in the wheat for the first time. The TaRLK-B is induced by a gibberellic disease in wanghuibai one variety of the wheat capable of resisting the gibberellic disease to be enhanced in expression, and the expression level of the TaRLK-B is much higher than that in Alondra's one variety of the susceptible wheat. The gene is inserted in pAHC25 to obtain an overexpression vector to convert yang wheat 158 one variety of the wheat affected by the gibberellic disease. After identification of T0-generation gibberellic disease resistance, results show that overexpression of the TaRLK-B can improve resistance of varieties of wheat affected by the gibberellic disease on the gibberellic disease.

The invention discloses a plant disease resistance-related protein and a coding gene and application thereof. The protein is (a) or (b): (a) a protein consisting of an amino acid sequence shown as the sequence 1 in a sequence table, and (b) a protein which is derived from the sequence 1, related to the plant disease resistance and obtained by substituting and / or losing and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1. When the coding gene of the protein is transferred into a plant, a transgenic plant with obviously improved disease resistance can be obtained. The plant disease resistance-related protein and the coding gene and application thereof have great significance for the cultivation of disease-resistant plants, particularly sheath blight-resistant and gibberella-resistant plants.

The invention relates to a heterocycle substituted 1,3,4-oxadiazole compound and a preparation method and application thereof. The compound includes the following steps shown in the general formula (I) in the description. The compound has a good inhibitory effect on pathogenic bacteria and fungi, such as rice white leaf diseases, citrus canker diseases, pepper wilt pathogenic bacteria, blueberry phytophthora lateralis, wheat gibberella saubinetii, potato phytophthora infestans, oilseed rape sclerotinia sclerotiorum, dragon-fruit colletotrichum orbiculare and the like.

The invention relates to a preparation method for a carbazolylv isopropanolamine derivative with the chiral center and application. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwi fruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteria and the like.

The invention discloses a quinoline compound and a preparation method and application thereof to prevention of plant diseases. The test result shows that the compound has the excellent prevention effects on plant diseases caused by cotton oxysporum, gibberella saubinetii, sclerotinia scleotiorum, rhizoctonia solani and rice blast and pumpkin powdery mildew and cucumber powdery mildew. The quinoline compound is simple in preparation technology, the raw materials are cheap and easy to obtain, the product purity is high, and the quinoline compound can be hopefully developed as a new bactericide.

The invention discloses an organic leaf fertilizer and a preparation method of the organic leaf fertilizer. The organic leaf fertilizer is prepared from the following raw materials in terms of parts by weight: 44-46 parts of urine, 14-16 parts of rhizobium astragalus, 20-22 parts of rape pollen, 13-15 parts of maize seeds, 8-10 parts of withered flowers, 12-14 parts of bleeding sap, 5-7 parts of brown sugar, 13-15 parts of willow bark, 2-4 parts of gibberella, 11-13 parts of stonewort, 6-8 parts of calcium hydroxide, 12-14 parts of pistia stratiotes, 7-9 parts of tea seed cakes, 3-5 parts of collophanite and 1-3 parts of polyhalite. The organic leaf fertilizer has the advantages of quickness in fertilizer efficiency, good fertility, insect pest resistance and capability of significantly increasing crop yield.

The invention belongs to the field of biotechnology, and particularly relates to a method for converting 3-cyanopyridine into nicotinic acid by using gibberella intermedia CA3-1. The strain is collected at the China General Microbiological Culture Collection Center with a collection number of CGMCC No. 4903. The strain can be used for converting the3-cyanopyridine into the nicotinic acid under the condition of substrate concentration of 5.2 g / L (50 mM), the substrate conversion efficiency reaches up to 98 percent; under the condition of the optimum reaction temperature of 30 DEG C, the half life reaches up to 231.1 h; and the nitrilase of the strain CA3-1 has a broad substrate spectrum, and various single nitrile and double nitrile compounds can be converted.

The invention discloses a method for identifying and evaluating wheat scab expansion resistance. The method is mainly characterized in that after wheat ears, gibberella spore liquid is injected into internode stems below ears and not dropped into florets, and the wheat scab expansion resistance is comprehensively evaluated according to occurring time of a first disease spikelet and the rate of the disease spikelet in the 22-25th day after inoculation. The method has the greatest advantages that moisturizing measures are omitted after inoculation in a large field or greenhouse environment, and the method solves the problem that a traditional single flower dripping method needs the moisturizing measures such as bagging or spraying and water spraying for identification. Compared with the traditional single flower dripping method, the method is higher in inoculation and lower in cost, disease prevalence rate is decreased, phenotypic difference resisting and sensing capacity of varieties is maximized, and common technicians can more effectively judge the scab expansion resistance of wheat varieties.

The invention discloses a wheat scab control method. The method includes 1, preparing selenium solution; 2, on a sunny day, spraying the selenium solution with the certain concentration on wheat in flowering period for 1 to 3 times through a spray device, allowing the wheat to serve as a treatment group, and spraying water on wheat serving as a control group; 3, on a sunny day for 3 to 5 days after flowering, namely in the early filling period, spraying the selenium solution on the treatment group through the spray device for 1 to 3 times, and spraying water on the control group; 4, recording the gibberella expanding speed on panicle portions of the treatment group and control group; 5, recording the diseased panicle rates of the treatment group and control group after 21 to 25 days after flowering; 6, recording grain infection rate after harvesting. The gibberella expanding speed on the panicle portions and the grain infection rate can be decreased significantly, and the wheat nutritional value can be increased and human, animal and plant health can be promoted through proper application.

The invention discloses a gibberellin-containing hydroponic leaf vegetable organic nutrient solution and a preparation method of the nutrient solution. The nutrient solution comprises the following material components in percentage by weight: 20 percent of sword beans, 20 percent of rice bran, 20 percent of water hyacinth, 10 percent of bone meal, 10 percent of potato vines, 10 percent of cucumber stems and 10 percent of tomato leaves and consists of fermentative microflora I and fermentative microflora II. The preparation method is characterized by comprising the following steps: preparing biological gibberellin from tender pods of sword beans by utilizing a fermentation process, matching with nitrogen, phosphorus and potassium and other trace elements in the main materials, fermenting to form the nutrient solution needed by leaf vegetables, fermenting by virtue of the fermentative microflora, thereby obtaining an active enzyme. A gibberellin growth regulator gibberellic acid (GA3) contained in the nutrient solution disclosed by the invention belongs to an active substance enzyme, effective absorption of plants on the active gibberella is improved, the yield of the cultivation target is increased, the nutritional requirement on full growth of the leaf vegetables can be met by virtue of a nutrition formula, the development of the root system and leaf surface is promoted, and the piece weight and quality of finished plants are increased.

The invention relates to a sterilization composition containing metconazole and albendazole. The effective active ingredients of the sterilization composition comprise the metconazole and the albendazole. When the weight ratio of the metconazole and the albendazole is 40-1:1-40, the sterilization composition has the synergistic effect on inhibition of growth of gibberella saubinetii. The sterilization composition is wide in bacteriocidal spectrum. Preparations processed according to different proportions can be used for controlling wheat scab, powdery mildew, the rust disease, grey mould fruit rot of strawberries and the rice sheath blight disease, the control efficiency of the sterilization composition to different diseases can reach more than 75%-99%, and the sterilization composition has the advantages of having fast-acting property and being good in persistence, and can improve the wheat yield and the wheat quality. The sterilization composition is novel in action mechanism and multiple in acting site, and can govern pesticide resistance of the gibberellic disease to carbendazim and erysiphe cichoracearum to triadimefon and delay occurrence and development of pesticide resistance of pathogenic bacteria to triazole bactericides.

The invention relates to a sterilization composition which contains metconazole and JS399-19 (the chemical name of which is 2-cyan-3-amino-3-ethyl-phenylacrylate and the test number of which is 'JS399-19'), which serve as effective active components. When the weight ratio of the metconazole to the JS399-19 is (1-40):(40-1), a synergistic interaction effect can be achieved on suppression of the growth of gibberella saubinetii. The composition has a wide bacteriocidal spectrum and is mainly used for preventing and treating the wheat scab, the powdery mildew and the rust disease, and a preventing and treating effect is outstanding; the preventing effect is stabilized over 80 percent; the sterilization composition has the advantages of high fast-acting property and high persistence; furthermore, the yield of wheat can be increased, and the quality of the wheat can be improved. A working mechanism of the sterilization composition is novel; the number of working sites is large; the sterilization composition can treat the drug resistance of gibberella saubinetii to carbendazim as well as the powdery mildew to triadimefon; the generation and the development of the drug resistance of pathogenic bacteria to a triazole fungicide are delayed.

The invention discloses Fusarium fujikuroi GA-251 and applications thereof, wherein the Fusarium fujikuroi GA-251 is a Gibberella fujikuroia mutant strain and has high yield of gibberellin GA3. The Fusarium fujikuroi GA-251 is preserved in China Center for Type Culture Collection, wherein the address is Wuhan University, Wuhan, China, the postal code is 430072, the preservation number is CCTCC NO:M 2019201, the preservation date is March 25th, 2019. The strain is obtained by carrying out radiation composite mutagenesis on protoplasts through lithium chloride and cobalt 60, and gibberellin GA3production of the strain is improved by 20%. The method has the advantages of high titer and easy extraction of the final product, and overcomes the defects of low fermentation titer and high cost oforiginal processes.

The invention provides a polysubstituted pyrido (4, 3-d) pyrimidine compounds with bactericidal activity and a synthesis method thereof. The structural general formula of polysubstituted pyrido (4, 3-d) pyrimidine compounds of the invention is showed in general formula I which includes three compounds as showed in general formulas I-1, I-2 and I-3; the proposed structure of general formula I-1 is the development and complement of Patent CN 100335481C while no relevant coverage on the structure of the general formulas I-2 and I-3 is released currently. The experiments indicate that the compounds with the general formula I can obviously inhibit various pathogenic bacteria such as fusarium oxysporum vasinfectum, rhizoctonia solani, botrytis cinera pers, gibberella saubinetii, ring rot of apple, cotton anthracnose, cucumber timberrot, cucumber target leaf spot, scab of cucumber, pepper phytophthora blight, etc., and can be used as the active ingredient of bactericides.

The invention discloses angelica sinensis endophytic fungi Fusella sp DG09 with plant pathogenic bacteria resistant activity and fermentation solution and application thereof. The Fusella sp DG09 was collected in China General Microbiological Culture Collection Center on January 7th, 2011, and has the collection number of CGMCC No.4543. The fermentation solution of the Fusella sp DG09 is prepared by the following method of: inoculating slant strains of the Fusella sp DG09 to a seed culture medium, performing shaking culture till an exponential growth phase, inoculating 10 percent of the cultured strains to a fermentation medium, culturing the strains for 4 to 5 days, performing centrifugation after the fermentation is finished to obtain supernate, and concentrating the supernate to obtain the fermentation solution. By numerous experiments, the endophytic fungi Fusella sp DG09 with strong plant pathogenic bacteria resistant activity of resisting gibberella saubinetti, early tomato phytophthora, tomato grey mould, rice sheath blight and the like is screened from angelica sinensis; and the endophytic fungi Fusella sp DG09 has good application prospect in biological prevention and control of plant diseases, and has important guidance significance for culture, production and sustainable utilization of medicinal materials such as the angelica sinensis and the like.

The invention relates to a preparation method for carbazolyl isopropanolamine derivative and application thereof. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwifruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteriaand the like.

The invention discloses a hydroxyquinoline compound as well as a preparation method and application thereof in preventing and treating agricultural diseases. Testing results show that the compound hasremarkable activity upon corn leaf fulvia fulvum, cotton oxysporum, potato rhizoctonia solani kuhn, didymella bryoniae, fusarium oxysporum, wheat gibberella, rice blast, botrytis cinerea, lycium barbarum anthracnose, cotton anthracnose, sclerotinia sclerotiorum, pepper phytophthora blight, potato late blight, rhizoctonia solani, melon bacterial fruit plaque bacteria, grape meloidogynosis bacteria, Chinese cabbage erwinia carotovora, kiwi fruit anabrosis bacteria, pseudomonas lachrymans, ralstorinia solanacearum, rice bacterial leaf blight and rice bacterial stripe bacteria. The hydroxyquinoline compound is simple in preparation process, cheap and easy in raw material obtaining, high in product purity, good in bioactivity and wide in sterilization spectrum and has the potential of being developed as new fungicides.

The invention relates to the genetic engineering field, especially to a novel gibberella Gibberela.sp. F75 with a preservation number CGMCC No.2499, and an acidic alpha-galactosidase Aga-F75 of antiprotease from said strain, further a gene thereof and a recombinant vector containing said gene. The amino acid sequence of the alpha-galactosidase Aga-F75 is shown as SEQ ID NO.1. The enzyme has a proper action pH value, a stronger protease resistance and a better hydrolysis ability for various substrates and is used for the feed and food industries as a feed or food additive agent.

The invention belongs to the field of application of bioengineering technology, and particularly relates to a method for preparing nicotinic acid by immobilizing nitrilase-producing Gibberella CA3-1 (Gibberella intermedia, CGMCC No. 4903) with polyvinyl alcohol-chitosan and sodium alginate-gelatin composite materials and crosslinking respectively with glutaric dialdehyde and genipin. The genipin is used for preparing immobilized cells for the first time to enhance the mechanical properties of the immobilized cells. The method comprises the following steps: immobilizing Gibberella CA3-1, adding a proper amount of 3-cyanopyridine, and reacting under the optimal reaction conditions to obtain the product nicotinic acid. The research detects that the chitosan-polyvinyl alcohol immobilized cells crosslinked by the genipin have better activity retentivity and reutilization property than immobilized cells crosslinked by glutaric dialdehyde, and have higher activity than the sodium alginate-gelatin composite material; the chitosan-polyvinyl alcohol immobilized cells crosslinked by the genipin can be reutilized repeatedly, have higher substrate tolerance, and can enhance the production capacity of unit thalli. The method provides technical support for continuous production of nicotinic acid from 3-cyanopyridine by using the Gibberella CA3-1.

The invention discloses a desulfurization, denitrification and carbon reduction agent and a preparation method and an application thereof. The preparation method of the desulfurization, denitrification and carbon reduction agent comprises the following steps: carrying out reaction on bio-industrial waste residue for 2-4 days in water under the action of microbial bacteria for obtaining a mixed product, then filtering the mixed product, and taking filtrate, wherein the microbial bacteria comprise alcaligenes sp and one or more of the following microbial bacteria: penicillium sp, gibberella, colistin sulphate and microzyme; and the cell concentration of the microbial bacteria is 1.3 multiplied 102-8.9 multiplied by 103 / mL. Filter residue obtained after filtering the mixed product which is prepared by the raw material bio-industrial waste residue and / or the method is further reacted with water solution of alkali, thereby obtaining the solid desulfurization, denitrification and carbon reduction agent. The main raw materials of the desulfurization, denitrification and carbon reduction agent are waste materials in other fields, thereby having low cost, hardly increasing ash content in the preparation of gasification briquettes, further effectively realizing desulfurization, denitrification and carbon reduction, and having the same significant effects when being used for desulfurization, denitrification and carbon reduction of flue gas, formed coke or formed iron of electric power plants.

The present invention relates to modified filamentous fungal organisms having improved activity profiles with respect to the conversion of complex carbohydrates into simple sugars from cellulosic materials, including fungal organisms belonging to a genus selected from the group consisting of: Chrysosporium, Thielavia, Talaromyces, Thermomyces, Thermoascus, Neurospora, Aureobasidium, Filibasidium, Piromyces, Corynascus, Cryplococcus, Acremonium, Tolypocladium, Scytalidium, Schizophyllum, Sporotrichum, Penicillium, Gibberella, Myceliophthora, Mucor, Aspergillus, Fusarium, Humicola, Trichoderma, and Talaromyces, plus anamorphs and teleomorphs thereof. Filamentous fungal organisms having improved activity profiles are obtained by modifying genes encoding enzymes involved in the production of cellobionolactone, cellobionic acid, gluconolactone, gluconic acid, and related products, by a variety of mutagenic methods, resulting in nucleotide substitutions, insertions, and deletions, increasing the level of saccharification in enzyme mixtures obtained from the modified organisms.

The invention discloses a preparation method of tebuconazole suspension concentrates used for preventing crop rust, powdery mildew, net blotch, foul foot and gibberella zeaze petch of wheat and barley, groundnut early leaf spot, exobasidium vexans massee of tea plants, and bunt smut. The preparation method comprises the following steps of dispensing, emulsifying, sanding, mixing, secondary-dispensing, stirring, canning and the like, wherein all components are prepared at the first dispensing according to a certain weight. The invention prevents products from layering, degrading and agglomerating, and has the advantages of good product stability, good use effect, low production cost, high drug effect, and good economic benefit.

The present invention provides an alkaloid compound that is an inhibitor of mycotoxin biosynthesis. The alkaloid is an alkenyl piperidine amide wherein the alkenyl is a C18 alkenyl with one or more double bonds. The alkaloid inhibits transcription of the fungus genes nor-1, tri5, and ipnA. The present invention further provides a method for identifying compounds, which inhibit biosynthesis of aflatoxin in Aspergillus spp. and deoxynivalenol in Gibberella spp. without inhibiting growth of the fungus in vitro.

The invention belongs to the biotechnology field, and particularly relates to a method for converting a substrate 4-AD into 11alpha, 15alpha-diOH-4-AD through Gibberella intermedia CA3-1. The bacterial strain is preserved in the Common Microorganism Center of China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC No. 4903. The 4-AD can be converted into 11alpha, 15alpha-diOH-4-AD through the Gibberella intermedia CA3-1; when the substrate feed concentration is 4 g / L, the 4-AD conversion rate reaches up to 99.3%, and the yield of the 11alpha, 15alpha-diOH-4-AD is 68.7%. The synthesis of the 11alpha, 15alpha-diOH-4-AD provides a more diversified precursor for research of steroid drugs and intermediates thereof, and the certain research value and market significance are achieved.

The invention discloses a Yangbi walnut germin-like protein gene JsGLP1 and an application thereof. The nucleotide sequence of the JsGLP1 gene is as shown in SEQ ID NO:1, and is capable of encoding germin-like protein. Through functional genomics related technological research, the gene JsGLP1 is proved to have the function of enhancing plant fungal infection resistance; the antifungal gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for over expression; the transgenic tobacco plant is strong in the in-vitro antifungal activity; and JsGLP1 over-expression transgenic tobacco has a significant inhibitory effect on the growth of sclerotinia sclerotiorum, beaded gibberella, colletotrichum gloeosporioides and fusarium oxysporum.