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Angelica sinensis endophytic fungi Fusella sp DG09 with plant pathogenic bacteria resistant activity and fermentation solution and application thereof

A technology of plant pathogenic bacteria and endophytic fungi, applied in the direction of plant growth regulators, botany equipment and methods, applications, etc., to achieve the effects of reasonable process design, environmentally friendly preparation methods, and industrialized production

Inactive Publication Date: 2011-09-07
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the antibacterial activity of endophytic fungi of medicinal plants. The research on Angelica sinensis mainly focuses on the research on active ingredients and pharmacological activity. There are no relevant reports on the research on endophytic fungi of Angelica sinensis. On the basis of this method, making full use of the resources of Angelica sinensis, looking for endophytic fungi and their metabolites with antibacterial activity, and developing them into biopesticides for the prevention and treatment of plant diseases and insect pests has important application prospects

Method used

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  • Angelica sinensis endophytic fungi Fusella sp DG09 with plant pathogenic bacteria resistant activity and fermentation solution and application thereof
  • Angelica sinensis endophytic fungi Fusella sp DG09 with plant pathogenic bacteria resistant activity and fermentation solution and application thereof
  • Angelica sinensis endophytic fungi Fusella sp DG09 with plant pathogenic bacteria resistant activity and fermentation solution and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A fermented liquid of angelica endophyte Fusella sp DG09 with anti-phytopathogenic activity, which is prepared by the following method:

[0026] Get the slant strain of Angelica sinensis endophytic fungus Fusella sp DG09 and inoculate to seed culture medium (seed culture medium is made by the raw material of following parts by weight: 2% glucose, 0.3% peanut cake powder (get peanut cake powder, add appropriate amount distilled water, boiled for 30 minutes, filtered, and got the filtrate), 0.3% magnesium sulfate, 0.3% potassium dihydrogen phosphate, 0.3% ammonium chloride) in shake flasks, at 28°C, 200r / min shaking culture until the logarithmic growth phase, The cultured bacterial classification is inoculated into fermentation medium (fermentation medium is made from the raw material of following parts by weight by 10% inoculum size: 1% soluble starch, 1% glucose, 0.3% magnesium sulfate, 0.3% phosphoric acid Potassium dihydrogen, 0.3% ammonium sulfate, 2% bean cake powde...

Embodiment 2

[0028] A fermented liquid of angelica endophyte Fusella sp DG09 with anti-phytopathogenic activity, which is prepared by the following method:

[0029] Get the slant strain of Angelica sinensis endophytic fungus Fusella sp DG09 and inoculate to seed culture medium (seed culture medium is made by the raw material of following parts by weight: 2% glucose, 0.3% peanut cake powder (get peanut cake powder, add appropriate amount distilled water, boiled for 30 minutes, filtered, and got the filtrate), 0.3% magnesium sulfate, 0.3% potassium dihydrogen phosphate, 0.3% ammonium chloride) in a shaking flask, at 28°C, 200r / min shaking culture until the logarithmic growth phase, The cultured bacterial classification is inoculated into fermentation medium (fermentation medium is made from the raw material of following parts by weight by 10% inoculum size: 1% soluble starch, 1% glucose, 0.3% magnesium sulfate, 0.3% phosphoric acid Potassium dihydrogen, 0.3% ammonium sulfate, 2% bean cake po...

Embodiment 3

[0031] An active part of Angelica sinensis endophytic fungus Fusella sp DG09 fermentation broth with anti-phytopathogenic activity, which is prepared by the following method:

[0032] Get the slant strain of Angelica sinensis endophytic fungus Fusella sp DG09 and inoculate to seed medium (seed medium formula is: 2% glucose, 0.3% peanut cake powder (get peanut cake powder, add appropriate amount of distilled water, boil 30 minutes, filter , take the filtrate), 0.3% magnesium sulfate, 0.3% potassium dihydrogen phosphate, 0.3% ammonium chloride) shake flask, at 28 ℃, 200r / min shaking culture to the logarithmic growth phase, the cultured strains are pressed 10% of the inoculum size is inoculated into the fermented medium (the fermented medium formula is: 1% soluble starch, 1% glucose, 0.3% magnesium sulfate, 0.3% potassium dihydrogen phosphate, 0.3% ammonium sulfate, 2% bean cake powder ( Take bean cake powder, add appropriate amount of distilled water, boil for 30 minutes, filter...

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Abstract

The invention discloses angelica sinensis endophytic fungi Fusella sp DG09 with plant pathogenic bacteria resistant activity and fermentation solution and application thereof. The Fusella sp DG09 was collected in China General Microbiological Culture Collection Center on January 7th, 2011, and has the collection number of CGMCC No.4543. The fermentation solution of the Fusella sp DG09 is prepared by the following method of: inoculating slant strains of the Fusella sp DG09 to a seed culture medium, performing shaking culture till an exponential growth phase, inoculating 10 percent of the cultured strains to a fermentation medium, culturing the strains for 4 to 5 days, performing centrifugation after the fermentation is finished to obtain supernate, and concentrating the supernate to obtain the fermentation solution. By numerous experiments, the endophytic fungi Fusella sp DG09 with strong plant pathogenic bacteria resistant activity of resisting gibberella saubinetti, early tomato phytophthora, tomato grey mould, rice sheath blight and the like is screened from angelica sinensis; and the endophytic fungi Fusella sp DG09 has good application prospect in biological prevention and control of plant diseases, and has important guidance significance for culture, production and sustainable utilization of medicinal materials such as the angelica sinensis and the like.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to an Angelica sinensis endophytic fungus Fusella sp DG09 with anti-phytopathogenic activity, Fusella sp DG09 fermented liquid and its preparation method and application. Background technique [0002] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, a plant of the Umbelliferae family. It is sweet, pungent, and warm, and it returns to the liver, heart, and spleen meridians. Angelica medicinal materials are mainly produced in Minxian, Wudu, Chengxian and other places in Gansu, my country. It is a commonly used traditional Chinese medicinal material, and resources are relatively scarce. Roots and rhizomes such as angelica and ginseng are particularly susceptible to infection by harmful bacteria during the cultivation process. The annual yield reduction due to plant diseases reaches 10-30%, and in severe years it can reach more than 50%. decline. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 江曙段金廒钱大玮陶金华
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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