The invention relates to an artificial feed of a rice leaf folder and a preparation method of the artificial feed. The artificial feed belongs to the technical field of insect raising. The formula is prepared from the following components of water, rice leaf powder, wheat germ powder, corn meal, soybean meal, casein, granulated sugar, agar, yeast, mildew inhibitor, antioxidant, insect feed oil, antibiotic, choline chloride, wade-salt, cholesterol and vitamine. The mildew inhibitor used in the formula is composed of 2-3 of natamycin, sorbic acid, nipagin, tebuconazole and azoxystrobin; the antioxidant is mixture of ascorbic acid and phospholipid; the insect feed oil is the mixture of linseed oil, rice bran oil and germ oil, and the antibiotic may be streptomycin or chloramphenicol or aureomycin. Survival rates of larvas can be obviously improved by raising the rice leaf folder by the feed in the formula, and a foundation is created for an indoor research of the rice leaf folder.