The invention relates to a separation and purification method of endophytic fungi from
ginkgo biloba. The separation and purification method comprises the following operation steps of: (1) preparing
ginkgo biloba tissues: collecting roots, stems and leaves of the
ginkgo biloba and preserving under the condition that the temperature is 4 DEG C; (2) performing surface disinfection and sterile detection on the
ginkgo biloba tissues; and (3) performing separation culture on the endophytic fungi from the
ginkgo biloba tissues. The endophytic fungi from the
ginkgo biloba, described by the invention, is named as Fusariumsolani T-7 and currently collected in China General
Microbiological Culture Collection Center designated by State
Intellectual Property Office, the collection data is July 20, 2011, and the collection number is CGMCC No. 5089. The endophytic fungi from the ginkgo biloba, disclosed by the invention has the
microbiology position of the Fusariumsolani T-7 with broad-spectrum
antimicrobial activity, and the strain is simultaneously found to have an obvious effect of inhibiting the growth of mycelia against pathogenic fungi of six tested plants. According to the separation and purification method disclosed by the invention, resources of the
plant endophytic fungi are utilized to get a novel agricultural antibiotic type natural component for resisting diseases caused by the
plant pathogenic fungi.