The present invention relates to a preparation method of antibacterial and antifungal, styrene-butadiene-rubber, foamed sheet. Using 62-70 % SBR as basic material, 14-20% SIS, 0.6-1.2% antibacterial agent, 0.5-1.0% antifungal agent, 5-8% naphthenic oil, 6-10% clay, 1.4-2.2% OBSH, 0.5-1.0% sodium butyl dithiocarbonate, 0.5-1.0% DCP, and 0.6-1.2% glyceryl monostearate are added, after passing through plasticization, twin-roll mixing, first moulding foaming, cooling, second moulding foaming, cooling pitching, and punching shear, the production is formed. The foamed sheet prepared by this method, has low density, high impact resistance, good compression reducibility, wear resistance and slip resistance, good damping effect, no poison and no smell, especially, has excellent antibacterial and antifungal ability, is suitable for the floor board of indoor fitness and exercise sites, and can effectively inhibit pathogenic bacteria and mildew's reproduction and living, thus improves the sanitarian condition of the fitness and exercise sites.