2599 results about "Antibacterial peptide" patented technology

Antimicrobial peptides enable an alternate approach to developing antimicrobial coatings due to their targeting of the membranes of the bacteria. High specific activity is achieved by orienting the peptides so that the antimicrobial ends of the peptides maximally contact the bacteria. In one embodiment, one end of the peptide is covalently attached directly to the substrate. In another embodiment, the peptides are immobilized on the substrate using a coupling agent or tether. Non-covalent methods include coating the peptide onto the substrate or physiochemically immobilizing the peptides on the substrate using highly specific interactions, such as the biotin / avidin or streptavidin system. The compositions are substantially non-leaching, antifouling, and non-hemolytic. The immobilized peptides retain sufficient flexibility and mobility to interact with and de endocytosed by the bacteria, viruses, and / or fungi upon exposure. Immobilizing the peptides to the substrate reduces concerns regarding toxicity of the peptides and the development of antimicrobial resistance, while presenting substantially all of the peptide at the site of action at the surface of the substrate.

The invention discloses a high-activity antibacterial peptide-containing matrix for garden seedling cultivation, as well as a preparation method and application thereof. The high-activity antibacterial peptide-containing matrix for garden seedling cultivation is prepared from the following components: Chinese medicament residues, organic additives, inorganic additives, microbial blends, housefly larva excrement, functional microbes and a water retention agent, wherein the functional microbes have the effects of activating matrix nutrition, exogenously fixing nitrogen, antagonizing pathogenic microorganism, reinforcing absorbing capability of seedling roots to nutrition and promoting seedling growth. Furthermore, the matrix for garden seedling cultivation has comprehensive nutrient elements, stable performance, strong buffer capability, and good water and fertilizer retaining performance; and the preparation method has simple production process, short production period, wide sources and low cost of raw materials, no environmental pollution and convenience in large-scale commercial production. The matrix for garden seedling cultivation can be used for raising and cultivating garden green seedlings, and can also be used for repairing and improving the ecological environment.

The present invention relates to a composition including an effective amount of at least one of an antimicrobial peptide and a substance having an antimicrobial peptide effect and an effective amount of a neurotrophin. The composition can also include an effective amount of at least one of a growth factor and a neuropeptide. The present invention also relates a method of treating an injury to a nervous system of an animal that includes the steps of identifying the injury to the nervous system and applying to the injury an effective amount of at least one of antimicrobial peptide and a substance having an antimicrobial peptide effect. The method can also include applying an effective amount of one or more trophic factors selected from the group consisting of a growth factor, a neurotrophin, and a neuropeptide to the injury.

A nanoparticle includes a carbohydrate carrier and a bacteriocin. A method for prolonging efficacy of a bacteriocin against a food pathogen includes providing the bacteriocin in a delivery system, and inhibiting the food pathogen by the bacteriocin. A duration of efficacy of the bacteriocin against the food pathogen when the bacteriocin is provided in the delivery system exceeds a duration of efficacy of the bacteriocin when the bacteriocin is provided without the delivery system.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg / g, the reducing sugar reaches 20-80mg / g, the GABA reaches 0.317-0.5mg / g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu / g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU / g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

The invention discloses cathelicidin-BF and a gene and application thereof, which belong to the field of biomedicine. The cathelicidin-BF is straight chain polypeptide and contains thirty amino acid residues, the molecular weight is 3,637.54Da, and the isoelectric point is 11.79. The complete sequence of the cathelicidin-BF is lysine-phenyl alanine-phenyl alanine-arginine-lysine-leucine- lysine-lysine-serine-valine-lysine-lysine-arginine-lactamine-lysine-glutamic acid-phenyl alanine- phenyl alanine-lysine-lysine-proline-arginine-valine-isoleucine-glycin-valine-serine-isoleucine- praline-phenyl alanine. The gene for encoding the cathelicidin-BF consists of 750 ribonucleotides, wherein 484th to 573rd ribonucleotides are used for encoding a mature peptide part. The cathelicidin-BF has small molecular weight, strong sterilization effect, and quick action time, and has quite strong killing function to a plurality of kinds of clinical drug-fast bacteria. In addition, the cathelicidin-BF also has the advantages of broad-spectrum antibiotics, salt independence and so on.

A process was developed for expressing antimicrobial peptides in a recombinant host cell that eliminates host cell toxicity and antimicrobial peptide degradation, as well as providing a process for rapid purification. Fusion proteins comprising a periplasmic targeting signal, cleavage site, and antimicrobial peptides provide the basis for this process which produces antimicrobial peptides that may be used in antimicrobial compositions and articles.

The invention provides a lactobacillus plantarum ZJ316 which is separated from excrement of infants and anti-bacterial peptide which is produced by the fermentation of the lactobacillus plantarum ZJ316, namely plantaricin ZJ316, and preparation and application thereof; the plantaricin ZJ316 is a novel lactobacillus anti-bacterial peptide with high efficiency and small molecular; the anti-bacterial peptide has bacteriostatic action of broad spectrum; due to small molecular weight, a lipid bilayer is hard to be crossed so as to form a complex body with small holes to be different from the action mode of nisin; but the anti-bacterial peptide also has sterilizing capacity with high efficiency, is a lantibiotic anti-bacterial peptide with superhigh application and development potential and can be applied to preparing the alternative medicine of feed additives, food preservatives and antibiotic, etc.

The invention concerns agents with anti-bacterial activity and methods and intermediates for their production. The present invention further concerns the use of such agents for the treatment of bacterial infections in animals, including man. The agents are derivatives of vancomycin-type antibiotics, of structure: V-L-W-X; wherein V is a glycopeptide moiety which inhibits peptidoglycan biosynthesis in bacteria; L is a linking group; W is a peptidic membrane-associating element such as an element based on naturally-occurring animal or bacterial peptide antibiotics; and X is hydrogen or a membrane-insertive element.

The invention discloses a premix feed for relieving astriction of sows, which is prepared by mixing 5 to 7 weight parts of premix feed and 93 to 95 weight parts of main ingredient. The premix feed comprises the following components in part by weight: 0.8 to 1 part of liquoric root, 0.8 to 1.0 part of Bacillus subtillis and lactic acid bacteria preparation, 0.3 to 0.6 parts of antibacterial peptide and 1.2 to 1.6 part of choline chloride. The premix feed can relieve astriction of sows by adding a raw material with high crude fiber content or adding mineral substance aperients, microecological preparation, functional oligosaccharide, dietary fibers, antibacterial peptides and various green additives, thereby improving the production performance of sows.

The invention discloses a fresh shrimp freezing and antistaling agent as well as a fresh shrimp freezing and antistaling method. The antistaling agent is a mixture containing tea polyphenol, chitosan, antibacterial peptide, nisin, glucose oxidase, sodium chloride, lactic acid and trehalose. The antistaling agent is used for freezing and fresh-keeping of fresh shrimps; the fresh shrimp freezing and antistaling agent has natural components, and can be used for better protecting freshness of aquatic products, maintaining original appearance and state, reducing loss of proteins and other nutrients, inhibiting generation of bacteria, and substantially prolonging shelf-life and storage life.

The invention discloses a method for producing millet wine by using lactobacillus fementation. The method comprises steps of primary fermentation and secondary fermentation, and the secondary fermentation is that lactic acid bacteria are added in wine liquid to ferment, adding proportion of the lactic acid bacteria is 105-109CFU / mL, and the lactic acid bacteria are compositions of any one or more than two of lactic acid bacteria of oenococcus, lactic acid bacteria of lactobacillus, lactic acid bacteria of leuconostoc and lactic acid bacteria of pediococcus. The lactic acid bacteria are externally planted in the method, lactic acid and antibacterial peptide produced by the lactic acid bacteria can restrain movement of other bacteria, so that the lactic acid and the antibacterial peptide have inbibitional effects on rancidity, problems of reduced wine yield and damaged end product wine flavor of caused by rancidity are avoided, flavor of the obtained millet wine is special, and the method is favorable for product stability in an after-ripening process of the millet wine.

The invention belongs to the field of product extraction technology and relates to a method for extracting chitin and antibacterial peptide from fly larvae. The method comprises the following steps of: firstly disinfecting and rinsing fresh fly larvae to be clean, then extruding the cleaned fly larvae to separate the fly larvae skin from visceral mass serous fluid; firstly soaking with hydrochloric acid solution, drying after washing, and then soaking with NaOH solution to remove protein; retaining residual liquid, drying after cleaning, adding the NaOH solution after crushing, carrying out water bath at the temperature of 98 DEG C, and heating for reaction; putting chitosan into acetic acid solution, stopping reaction after the chitosan is dissolved, filtering, cleaning, drying at the temperature of 50 DEG C, and obtaining the chitosan; and then adding acetic acid into the visceral mass serous fluid of the fly larvae, homogenizing, standing over the night at the temperature of 4 DEG C, filtering, adsorbing the filtrate with alginic acid, filtering, then eluting the antibacterial peptide adsorbed on the alginic acid with the hydrochloric acid solution, adjusting the pH value of the eluent to be 3.5, carrying out precipitation with NaCl, carrying out dialysis and desalting, drying and obtaining a crude antibacterial peptide product. The method has the advantages of reasonable technical route, low cost, high yield, large economic value, environmentally-friendly effect and effective utilization of the fly larvae resources for preparing natural product varieties.

The invention relates to a core-shell nanofiber wrapping lipidosome and a preparation method and application thereof. The core-shell nanofiber is characterized in that the shell layer of the nanofiber is made from biocompatible polymer, the core layer of the nanofiber is made from natural polyelectrolyte, the lipidosome is wrapped in the core layer of the fiber and is loaded with active substances, the diameter of the core-shell nanofiber is 100-800 nm, and the core-shell nanofiber can control the active substance in the lipidosome to be slowly released to be balanced within 8-14 days. The core-shell nanofiber material prepared by the method has good biocompability, can control the release of growth factors, antibacterial peptide and various active drugs, is stable in performance and easy to store, and has wide application prospect.

Methods for controlling the growth of bacteria in ethanol fermentation systems with antibiotic alternatives, which can be nonoxidizing biocides, stabilized oxidizers, or any combinations thereof, are described. As an option, a process or composition of the present invention can include one or more polycyclic antibacterial peptides. The methods can provide improvements, such as increased ethanol yields with minimal carryover of biocide into co-products of the processes.

The invention provides a complex bacterium for bean pulp fermentation. The complex bacterium is composed of bacillus subtilis, bacillus coagulans, aspergillus niger, aspergillus oryzae, lactobacillus plantarum and saccharomyces cerevisiae. The complex bacterium provided by the invention is obtained through repeated scientific experiment and reasonable combination based on physiological metabolism feature of the microorganism, the complex bacterium is vigorous in growth metabolism, is capable of secreting a plurality of enzymes, acids and other metabolites, and is capable of efficiently decomposing macromolecule protein, anti-nutritional factors and sensitization factors in the bean pulp, obviously increasing content of soluble protein, small peptide, organic acid, protease and vitamin and the number of probiotics and antibacterial peptide, improving the digestion utilization rate, inhibiting the growth of pathogenic bacteria and reducing the use of antibiotic; and meanwhile, the complex bacterium has acid fragrance of fermentation and is good in food attractant effect. The bacteria strains forming the complex bacterium are included in a feed additive variety catalogue (2013) issued by the department of agriculture, the complex bacterium is safe and reliable, and can be directly used for the industrial production for the bean pulp fermentation.

The invention discloses a preparing method of east Asia Tityus antibiotic peptide gene and appliance, which comprises the following steps: restructuring bacillus coli Escherichia coli DH5a / BmKAMP1, CCTCC NO: M207036; constructing east Asia Tityus ioterium cell cDNA library; choosing PCR method; sieving positive colony of scorpion antibiotic peptide gene from ioterium cDNA library; sequence-analyzing coding trait of antibiotic peptide gene; assuring amino acid sequence of the antibiotic peptide gene; adopting chemosynthesis antibiotic peptide; possessing inhibition of diverse density for Gram's bacterium. This antibiotic peptide possesses specificity and high active, which can be used as antibacterial drugs.

The invention discloses non-resistant dust-free piglet creep formula feed and a preparation method thereof. The feed is prepared from the following components: corns, fermented corns, soybean meal, fermented soybean meal, expanded soybean flour, expanded corn flour, imported fish meal, spray-dried plasma proteins, dried porcine solubles, powdered whey, soybean oil, sucrose, molasses, sodium glutamate, a feeding promoting agent, Yibao phospholipid, yeast autolysate, an acidifying agent, stone powder, calcium hydrogen phosphate, table salt, 98.5 percent of lysine, 98.5 percent of threonine, 10 percent of tryptophan, special compound enzyme for early-weaned piglets, phytase enzyme, a micro-ecological preparation, a Chinese herbal medicine heath preserver, antibacterial peptide, immunopotentiator, 50 percent of choline chloride and a premix. The preparation method comprises the following steps: firstly, separately preparing granulated feed products and powdery feed products; and then mixing the granulated feed products with the powdery feed products to obtain the non-resistant dust-free piglet creep formula feed. According to the feed disclosed by the invention, antibiotics are not added, a formula is scientific, the dust of the product is low, the palatability and the immunity are improved, and a diarrhea ratio of piglets is reduced.

The invention discloses a safe compound feed of juvenile prawn of penaeus vannamei boone, which consists of bean pulp, pea albumen powder, fish powder, blood cell albumen powder, cuttlefish powder, shrimp shell powder, double-low rapeseed meal, cotton pulp, monocalcium phosphate, No. 1 flour, gluten powder, 50% choline chloride, complex vitamin for shrimp, microelement additive for shrimp, bacillus licheniformis, decorticating element, lower polyxylose, fermented peptide for aquatic product, gene recombinant protein growth promoter, nutrition immunology aquatic product food attractant, phospholipids oil and fish oil. With the gene recombinant protein growth promoter, the nutrition immunology aquatic product food attractant, and the high antibacterial peptide-containing fermented peptide, the safe compound feed can effectively restrain various enteropathogenic bacteria, improves the digestibility of the nutrition constituent of the feed, promotes the growth of the penaeus vannamei boone, can improve the organism immunity of the penaeus vannamei boone, and can obviously improve the survival rate of the penaeus vannamei boone.

The invention relates to a production method of bacillus subtilis antimicrobial lipopeptide and application thereof in feeds, belonging to the fields of feed additives and the biotechnology. The production method of the bacillus subtilis antimicrobial lipopeptide comprises the following steps of carrying out amplification culturing on an acillus subtilis fmbj seed culture medium, inoculating by 2-5 percent at the temperature of 28-35 DEG C, deeply fermenting 5000L of fermentor liquid for 30-48h, then extracting, and carrying out spray drying after concentration or directly carrying out spray drying to produce an antibacterial peptide product. The antimicrobial lipopeptide is added into basic daily feeds for broilers by a certain titer, thereby enhancing the feed utilization ratio and having better effect on growing development and immunity strengthening of broilers. The bacillus subtilis is a safe microorganism, and an antibacterial product thereof has high safety. In addition, compared with flavomycin used in the traditional broiler feeds, the bacillus subtilis antimicrobial lipopeptide has better effect and has an important significance on replacing feed antibiotic addition and guaranteeing food safety.

The invention discloses a green mud crab antibacterial peptide Sphistin and application thereof, relates to the technical field of genetic engineering of crustaceans, and provides the green mud crab antibacterial peptide Sphistin and the application thereof. A molecular formula of the green mud crab antibacterial peptide Sphistin is C170H293N61O46S1 and molecular weight of the green mud crab antibacterial peptide Sphistin is 3,959.61 Dalton. The antibacterial peptide Sphistin consists of 38 amino acids, is derived from a part of sequences at end N of green mud crab histone 2A protein and is apolypeptide with an antibacterial function. By adopting the conventional solid-phase chemical synthesis method, the green mud crab antibacterial peptide Sphistin with purity of over 95 percent can beobtained. The green mud crab antibacterial peptide Sphistin has an obvious antibacterial effect on various gram negative bacteria and gram positive bacteria, has a broad antibacterial spectrum and high sterilization rate, shows extremely high medical value and is excellently applied to preparing antibacterial agents.

The invention discloses a screening method of 79 antibacterial agents in animal foods. The screening method comprises preparation of a standard working solution, analysis pretreatment on a sample to be tested, drawing of a standard curve, qualitative analysis and quantitative analysis. A high throughput screening and quantitative detection platform is built so that the detection of a single compound is developed into the simultaneous detection of a plurality of compounds in the field. Compared with the prior art, the screening method realizes a detection cost about 1 / 2 the original cost, shortens a sample detection period by 1 / 2 and greatly improves work efficiency. The screening method plays an effective role in the veterinary drug residue monitoring program, the pollution-free animal product testing program and the national animal product routine testing program implemented by the Ministry of Agriculture.

The invention discloses a coated fabric containing antibacterial peptides and the preparation method, the surface of the fabrics are coated with coating agents containing antibacterial peptides. The preparation method comprises as follows: firstly, the antibacterial peptides are dissolved or dispersed into the coating agents at normal temperature to form finishing agents, then the fabrics are coated by the conventional rolling-baking method. The coated fabric containing antibacterial peptides has the advantages of broad spectrum antibacterial and bactericidal effect because the surface of the fabrics are coated with coating agents containing antibacterial peptides; no obvious influence on the appearance of the fabrics due to the thin coating layer.

The invention relates to synthetic antibacterial peptide comprising polypeptide selected from SEQ ID NO:1-SEQ IDNO:13 amino acid sequences, and polypeptide of a functional equivalent obtained from the synthetic antibacterial peptide through cyclization, N-terminal and C-terminal modification, translation from L-type amino acid to D-type amino acid, and sequence terminal deletion. The synthetic antibacterial peptide is synthesized by a solid phase chemical method. The synthetic antibacterial peptide has broad-spectrum killing activity to gram negative bacteria, gram positive bacteria and epiphytes, has stronger bactericidal activity than natural antibacterial peptide, has no toxic action on animal and plant cells, and can be used for preparing medicaments for curing infectious diseases.

Disclosed herein are novel antimicrobial peptides with useful, improved, or superior properties such as antimicrobial activity, desirable levels of hemolytic activity, and therapeutic index against a broad range of microorganisms including gram-negative and gram-positive bacteria and other organisms having a cellular or structural component of a lipid bilayer membrane. Also provided are methods of making and using such peptides to control microbial growth and in pharmaceutical compositions for treatment or prevention of infections caused by such microorganisms. Certain peptides are disclosed utilizing a structure-based rational design relating to an antimicrobial peptide, V681, with single D- / L-amino acid substitutions or charged residue substitutions in or near the center of the peptide on the nonpolar or polar face. Also disclosed are peptides with one or more amino acids in the D configuration, including peptides with all amino acids in the D configuration. Modified peptide analogs herein can demonstrate one or more properties such as improved antimicrobial activity, specificity, and resistance to degradation. Compositions disclosed herein have useful clinical potential as antibiotics including broad spectrum antibiotics.

The invention discloses microbial fermentation feed as well as a production method and an application thereof. The production method of the microbial fermentation feed comprises the following steps: (1) lactic acid bacteria, Clostridium butyricum Cb-2, yeast, Bacillus subtilis, a fermentation base-material and water are uniformly mixed, and a fermentation raw material is obtained; 2) aerobic fermentation and anaerobic fermentation are carried out in order for the fermentation raw material obtained in the step (1), and after fermentation, the feed is obtained. The microbial fermentation feed contains microecological viable organisms, and the total amount of beneficial bacteria is more than 5 x 10<9> cfu / g; the amount of mould and the content of mould toxin are lower than national limit standards; the feed is rich in organic acid, the content of lactic acid reaches 2.5%, and pH value is lower than 4.5; the titer of antibacterial peptide is higher than 1000 u / g, the titer of neutral protease is 150 u / g, the titer of alpha-amylase 300 u / g, dry weight for above, and the titer of total protease is higher than 600 u / g; the whole fermentation process is controlled in the range of 8-24 hours, the fermentation time is saved, and the dissipating energy is controlled below 3%.

The invention relates to antibacterial peptide active organic fertilizer and a preparation method thereof and belongs to the technical field of agricultural fertilizer. The fertilizer consists of livestock and poultry excrement, silkworm excrement and a microbial fermenting agent. The preparation method for the fertilizer comprises the following steps: 1) weighing the livestock and poultry excrement, the silkworm excrement and the microbial fermenting agent according to the weight ratio, mixing, adjusting the water content of the mixture to 55 to 65 percent, adjusting the pH value to be 5.0 to 8.0, and adjusting the C / N value to be 20 to 30; 2) performing primary stacked fermentation; 3) performing secondary stacked fermentation; and 4) screening the fermented organic fertilizer obtained in the step 3) to remove impurities, pelleting, shaping, discharging, drying, cooling, removing dust, detecting and packaging to obtain the antibacterial peptide active organic fertilizer. Compared with control, the antibacterial peptide active organic fertilizer can increase the yield by 20.1 to 25.3 percent when applied in the fields of fruits, vegetables, grain and oil, flowers and trees, tea and the like.

The invention provides self-assembly antibacterial peptide, and belongs to the field of self-assembly polypeptide.The sequence of the antibacterial peptide is Nap-Phe-Phe-Lys-Pro-Leu-Gly-Leu-Ala-Arg-Lys (Nap represents a naphthyl group, Phe represents phenylalanine, Lys represents lysine, Pro represents proline, Leu represents leucine, Gly represents glycine, Ala represents alanine and Arg represents arginine), and the antibacterial peptide is cationic amphiphilic oligopeptide.The antibacterial peptide has good self-assembly capacity, and the self-assembly behaviors present enzyme responsiveness.The antibacterial peptide has no toxicity to Gram negative bacteria, human cells and animal cells within a specific concentration range (50-150 micrometers), has powerful selective killing capacity only for Gram positive bacteria and has good antibacterial activity and selectivity in other words.

The invention relates to lactococcus lactis subsp.lactis, antibacterial peptide produced by lactococcus lactis subsp.lactis and the application of antibacterial peptide. In the invention, the collection number of the lactococcus lactis subsp.lactis LLC518 in the General Microorganism Center of China Microorganism Culture Collection Management Committee is CGMCC No. 4584. In the invention, the antibacterial peptide produced by the lactococcus lactis subsp.lactis can restrain micrococcus tetragenus, Bacillus thuringiensis, lactococcus lactis and listeria innocua, particularly, has strong restraint function to L. monocytogenes which can cause serious food poisoning. Thus, the lactococcus lactis subsp.lactis and the antibacterial peptide thereof have active function in food fresh keeping.