321 results about "Bacteriocin" patented technology

The present invention is generally related to a product and process to reduce the microbial contamination on organic matter, such as processed meat, fruits and vegetables, plant parts, inanimate surfaces such as textiles and stainless steel, and in the mouth or on dental products. In particular, the invention is related to a product and process to disinfect surfaces using an antimicrobial composition containing an antimicrobial lipid, an enhancer selected from the group consisting of bacteriocins, antimicrobial enzymes, sugars, sugar alcohols, iron-binding proteins and derivatives thereof, siderophores, and combinations thereof, and optionally a surfactant.

A nanoparticle includes a carbohydrate carrier and a bacteriocin. A method for prolonging efficacy of a bacteriocin against a food pathogen includes providing the bacteriocin in a delivery system, and inhibiting the food pathogen by the bacteriocin. A duration of efficacy of the bacteriocin against the food pathogen when the bacteriocin is provided in the delivery system exceeds a duration of efficacy of the bacteriocin when the bacteriocin is provided without the delivery system.

Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.

The present invention relates to probiotic Propionibacterium strains and their use in the preparation of probiotic supplements and foods. The invention relates to the provision of Vitamin B12, propionic acid, folacin and bacteriocins by probiotic strains, stimulation of bifidobacteria growth, production of favourable effects on the lipid metabolism and on the immune system of hosts through immunostimulation, immunomodulation or use of a probiotic strain as an adjuvant, reduction of homocysteine and β glucuronidase and the prevention, treatment or amelioration of conditions associated with a need for these activities. The probiotic bacteria of the invention can be used in humans or other animals. In at least some applications, the bacteria can be used dead and parts rather than whole cells may be used. The present invention also relates to the preparation of vaccines for use in protecting patients from infectious diseases, in particular tuberculosis.

The invention discloses a biological feed additive rich in bioactive peptides and probiotics as well as a preparation method of the additive. The biological feed additive prepared with the method has the peptide content higher than 20%, the active probiotic content up to 1.0*10<9> cfu / g and contains fermentation metabolites such as bacteriocin and the like; meanwhile, the additive has the advantages that the additive is purely natural, non-toxic, harmless and free of pollution and residues.

A stabilized cheese flavoring system which can be used to prepare very different cheeses having desired flavor profiles in which the flavoring system is stabilized against the growth of spoilage or pathogenic microorganisms therein, while flavor development can be accelerated in one more of its flavor components thereof. The stabilized flavoring systems are obtained by addition of a bacterocin source as part of fermentation process that accelerates the fermentation time needed for flavor development in one or more of its flavor components, and in at least a sulfur-cheddary component thereof in one embodiment. Therefore, production times can be significantly reduced for one or more of the flavor components of the flavoring system of the present invention without loss of flavor and while improving its microbial stability.

The invention provides bacillus subtilis LFB112 with the collection number of CGMCC No.2996. The bacterial strain has relatively high antibacterial activity and has antibacterial effect on various animal pathogenic bacteria and food pathogenic bacteria. The invention also provides bacteriocin produced by the bacterial strain, wherein the bacteriocin is relatively stable against heat and acid and has a broad-spectrum antibacterial effect without inhibiting beneficial bacteria. Experiments indicate that the bacterial strain LFB112 provided by the invention and the bacteriocin produced by the same can be used for improving the clinical symptoms of chicken and increasing the growth speed and feed price, and can be used in biological veterinary medicaments or feed additives.

The invention discloses an inactivated lactobacillus micro-ecological preparation and a preparation method thereof. The preparation comprises the following components in portion by mass: 10 to 60 portions of inactivated lactobacillus cell and lactobacillus fermentation product, and 30 to 50 portions of excipient, wherein the inactivated lactobacillus cell accounts for 10<8> to 10<9> cells per gram. The inactivated lactobacillus cell resists high temperature, gastric acid and bile, and has synergistic action with antibiotics; in addition, metabolic products produced in a lactobacillus fermentation process such as lactic acid, like bacteriocin and the like can effectively inhibit or kill enteric pathogenic bacteria of people or animals; and enzymes such as amylase, lactase and the like are helpful for digestion of the animals. The inactivated lactobacillus micro-ecological preparation effectively and economically solves the problem puzzling the prior viable bacteria micro-ecological preparation.

The present invention discloses a method for producing a fermented dairy product, preferably cheese, comprising inoculating milk with lactic acid bacteria capable of producing a Class IIa type bacteriocin; and lactic acid bacteria capable of producing a Class I type bacteriocin.

The invention provides a new Lactobacillus plantarum bacterial strain YJG which was preserved in the China General Microbiological Culture Collection Center (CGNCC) with the collection number of CGMCC No. 2994 on march, 30th, 2009. The Lactobacillus plantarum bacterial strain YJG can produce broad-spectrum bacteriostatic activity bacteriocin with high yield, and can express bacteriostasis for staphylococcus aureus, salmonella and E.coli of gram-negative bacterium, L. plantarum, L.brevis, Streptococcus and Bacillus of gram-positive bacterium, and the like. A virus attacking tests with high dosage proves that the Lactobacillus plantarum bacterial strain YJG is safe to animal bodies. The Lactobacillus plantarum bacterial strain YJG and a zymophyte liquid thereof can be used for preparing additives of green animal feeds, and the bacteriocin of the Lactobacillus plantarum bacterial strain YJG can be used for preparing green biological veterinary drugs, environmental-friendly biopreservatives, biocontrol drugs and the like.

A method for treating bacterial infections in a patient is provided. The method includes the step of administering a therapeutically effective amount of a single naturally-occurring or engineered bacteriocin, or combinations thereof, designed to have high specific activity against the bacterial infection to the patient so that a rate of resistance to the bacteriocin in the patient is decreased. The colicins and other bacteriocins or combinations of colicins and other bacteriocins target and kill specific bacterial pathogens in a manner that results in a high specific killing activity and decreased incidence of pathogen resistance. To this end, the characteristics of existing colicins and other bacteriocins are modified in order to enhance and amplify their therapeutic value.

The invention relates to a strain of lactobacillus plantarum and bacteriocins produced by the lactobacillus plantarum and capable of inhibiting Gram negative bacteria. The bacteriocins have the advantages of broad antibacterial spectrum, thermal stability and stable pH, degradability by protease, no residue in a human body and high safety. A lactobacillus plantarum strain is preserved on June 29, 2009 with a preservation number of CGMCC No.3151. A production strain is obtained by separating 'Jiaoke', a conventional dairy product in Inner Mongolia; on a MRS culture medium, colonies are ivory and round with a protruded center and orderly edges; the strain has two blunt round ends and a short and straight stem; and the size of the strain is 0.4 to 0.7 mu m *2 to 3 mu m. The strain is a non-spore Gram positive bacillus, and is identified as the lactobacillus plantarum through an API50CHL sugar alcohol fermentation test, a 16S rRNA sequence homology analysis test and a recA gene multiplex PCR method. The lactobacillus plantarum KLDS1.0391 is used as the production strain and is fermented and purified by using the improved MRS culture medium to obtain the bacteriocins of the lactobacillus plantarum. The bacteriocins are used in food preservatives.

The invention relates to a preparation method of (Lactobacillus planetarium subsp.plantarum)Zhang-LL and its listeria monocytogene-resistant bacteriocin. The preparation method is suitable for preservation and fresh-keeping of meat and meat products, milk and milk products, fruits and vegetable, and instant foods. The (Lactobacillus planetarium subsp.plantarum)Zhang-LL (CGMCC No.6936) is selected from bacon on the Fujian farmer's market. A bacteriocin production broth is obtained by fermentation of a Zhang-LL strain, and the Zhang-LL strain bacteriocin is extracted and purified by a pH-dependent adsorption-desorption method, a cation exchange chromatography and a reversed phase high-performance liquid chromatography so that titer is improved by 32 times and purity is improved by 36.65 times. The bacteriocin can inhibit a plurality of food-borne pathogenic bacteria such as listeria monocytogenes, has high bacteriostatic activity, good heat, acid and base stability, can be degraded by human protease and is a natural and safe biological preservative. The preparation method has the advantages of simple processes, stability, high efficiency, source convenience, low cost and industrial production feasibility.

The invention discloses a biological-selenium-enriched multi-probiotics fermented rice milk and a preparation method therefore, belonging to the field of food processing. Grains such as rice or broken rice, seeds of Job's tears, oat, and millet are used as raw materials, and the raw materials adopt a certain proportion of milk powder according to a principle of nutrition complementation. A modernprocess is used for researching and developing a multi-strain fermented rice milk product which has the particular flavor and active ingredients of yoghourt and also has the functional factors and flavor of the grains. The rice milk does not have any thickening agent, the gelatinization degree of a product is changed through a screw extrusion technology, and a suitable enzymolysis technology of different grains as the raw materials is optimized. A multi-strain co-fermentation mode is adopted to increase the enrichment of biological selenium and promote the metabolic quantity of bacteriocins, and the total strain content of the product in the quality guarantee period is increased.

The invention discloses bacillus licheniformis strain. Its preservation name is Bacillus licheniformis ZJU12. The preservation unit is Chinese typical culture preservation center. The data is July fourth in 2005 yare. The number is CCTCC M 205072. The invention also discloses the produced bacteriocin which has antagonism for gram-positive bacteria, plant pathogenic fungi, plant pathogeny gram-negative bacteria.

The invention relates to a bacillus amyloliquefaciens for inhibiting bird rape plasmodiophora brassicae and separation, application and purposes of active substances thereof. Bacillus amyloliquefaciens HB-26 is preserved in China Center for Type Culture Collection (CCTCC), the preserving number is CCTCCNO: M2011259. A culture method is as follows: inoculating biogas slurry separated bacterial strain in an LB liquid nutrient medium, preserving the biogas slurry separated bacterial strain at 30 DEG C for one night, pouring fermentation liquor at the root of Chinese cabbage and finding the bacterial strains with bacteriostatic activity on plasmodiophora brassicae. The separated active substance is characterized in that the HB-26 is inoculated in the LB liquid nutrient medium, the fermentation liquor is dried by freezing, the frozen strain powder is added with ethyl acetate for extraction, is vibrated, rotated and evaporated, is dissolved by methyl alcohol, and then subjected to HPLC (High Performance Liquid Chromatrography) separation, the components are collected, the mass spectrum identification is conducted, and the component of the active substance can be judged according to the condition of the generation of plasmodiophora brassicae disease of the Chinese cabbage. The molecular weight of the bacteriostatic activity substance BA20 is 1494.0, and the nuclear magnetic resonance hydrogen spectrum shows that the chemical nature of the bacteriostatic activity substance is carbohydrate bacteriocin. The active substance is used for preparing a microbial agent for preventing the plasmodiophora brassicae disease of the Chinese cabbage.

The invention relates to Bacillus amyloliquefaciens V4. The Bacillus amyloliquefaciens V4 is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC NO. 10149. The Bacillus amyloliquefaciens V4 strain and / or microbial agent can produce bacteriocin for antagonizing vibrio pathogens and pathogenic aeromonas at seedling growing and culture temperatures of common culture water, inhibit vibrio vulnificus, vibrio natriegen, vibrio harveyi, black sea vibrio, aeromonas hydrophila and aeromonas salmonicida growth and breeding, effectively control aquatic animal infectious disease generation and propagating caused by vibrio pathogens and pathogenic aeromonas, reduce aquatic animal mortality and a forage coefficient and promote aquatic animal weight gain.

A new kind of lactobacteria: pentose pediococcus YJL (BCRC910210) and pentose pediococcus YJS (BCRC 910211), the bacteriocin extracted from them for suppressing growth of other bacteria, the process for preparing the food from meat, fish and bean by use of them, and the resltant food with unique taste and long storage time are disclosed.

The invention relates to a preparation method of a bacteriocin-producing Lactobacillus plantarum subsp. Plantarum Zhang-LL active bacterial preparation, and belongs to the field of microbe application. In the preparation method, by using a bacteriocin-producing Lactobacillus plantarum subsp. Plantarum Zhang-LL CGMCC No. 6936 bacterial strain, the active bacterial preparation is prepared through activation, expanding culture, high-density fermentation, centrifugation, freeze-drying protective agent addition, a prefreezing process, a freeze-drying process and other processes. The preparation method comprises the following steps: firstly, rapeseed peptone is selected as a substituting nitrogen source, so that the culture condition of the lactobacillus plantarum is optimized while the cost of a culture medium is reduced; a 5L of full-automatic fermentation tank is used for performing the high-density fermentation, and under the optimized low-cost culture medium and the optimized culture condition, the viable count of fermented liquid can reach above 1010CFU / mL. In the preparation method, potato pulp is used as a matrix of a freeze-drying protective agent, and an optimal combined formula of the freeze-drying protective agent is determined, so that the survival rate of the Lactobacillus plantarum subsp. Plantarum Zhang-LL reaches above 60% and the viable content can reach above 1011CFU / g. The prepared active bacterial preparation is low in raw material price and relatively high in viable content, can provide a good foundation for developing a novel feed additive for poultry, and can provide a significant economic value.

The invention provides a new strain FM 603 of bacillus coagulans, and the preservation number of the strain is CGMCC NO.10221. The strain can generate bacteriocin antibacterial substances and achieves antibacterial activity for Gram-positive pathogenic bacteria such as Listeria monocytogenes, staphylococcus aureus, methicillin-resistant staphylococcus aureus, clostridium perfringens and clostridium difficile. The molecular weight of bacteriocin is 4276.45 Da, the partial amino acid sequence is Ala-Gly-His-Dhb-Phe-Val-Dhb-Gly-Pro, and the bacteriocin is stable under treatment of heat, acid, and pepsase or trypsin and can be degraded easily by pronase to be inactivated. The invention further provides an FM603 fermentation product which can be used as a microbiological feed additive. Animal test results show that the fermentation product can increase the laying rate of laying hens, reduce the feed-egg ratio and improve egg quality; the feed intake and daily gain of piglets are increased, and the feed conversion ratio is reduced; the daily gain and the content of lysozyme in serum of broiler chickens are increased, and the feed conversion ratio and the death rate are reduced.

The invention relates to a composite microbiological bacteriocin saline-alkali soil improver, and a preparation method and applications thereof. The composite microbiological bacteriocin saline-alkali soil improver comprises a microbial agent, an organic liquid and a matrix. The microbial agent specifically comprises bacillus subtilis and bacillus megaterium, and nutrient substances of protease, lipase, alfa-amylase, amino acid and the like can be genereated by fermentation of the microbial bacteriocins; and the microbial agent can be used to adjust soil acidity-alkalinity, reduce saline-alkali toxicity, adjust rice root system microbial environment, accelerate transition of trace elements comprising nitrogen, phosphorus and potassium, and accelerate root growth, root increase, seedling development and seedling strengthening, and helps to lay a good foundation for increase of rice production.

The invention relates to a composite microbiological bacteriocin saline-alkali soil improver, and a preparation method and applications thereof. The composite microbiological bacteriocin saline-alkali soil improver comprises a microbial agent, an organic liquid and a matrix. The microbial agent specifically comprises bacillus subtilis and bacillus megaterium, and nutrient substances of protease, lipase, alfa-amylase, amino acid and the like can be genereated by fermentation of the microbial bacteriocins; and the microbial agent can be used to adjust soil acidity-alkalinity, reduce saline-alkali toxicity, adjust rice root system microbial environment, accelerate transition of trace elements comprising nitrogen, phosphorus and potassium, and accelerate root growth, root increase, seedling development and seedling strengthening, and helps to lay a good foundation for increase of rice production.

Lysostaphin is a bacteriocin secreted by S. simulans to kill S. aureus, and has been shown to also be a potent antimicrobial for many antibiotic-resistant strains of S. aureus. By adding a ˜13 amino acid protein transduction domain (PTD) from the HIV-TAT protein to lysostaphin to form lysostaphin-PTD, both extracellular and intracellular forms of S. aureus and MRSA are killed in all (multiple) cell types examined.

The invention relates to a bifidobacterium BB2 that is separated from the alimentary canal of healthy broilers and can generate bacteriocin or bacteriostatic substances, and the collection number is CGMCC No. 3200. The strain has the advantages of strong stress resistance, rapid growth, high acidness of fermentation liquor, large biomass, bacteriostatic or antibacterial activity and the like. Theinvention also relates to a large-scale fermentative culture method of the strain, and bactericide with viable count of 12-15 billion / g can be obtained by centrifuging the fermentation liquor to recover bacterial sludge and adding protective agent after freeze drying. The bifidobacterium subspecies BB2 preparation can partially substitute for antibiotics, eliminate drug residues, improve the quality of animal products, animal productivity and immunity or disease resistance, reduce the mortality and elimination rate of animals and diarrhoea rate, and also purify the environment of animal houses. The strain, the method and the application have high social, economic and ecological benefits.

Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.

Novel Enterococcus and Streptococcus bacteriocins produced by novel Enterococcus and Streptococcus strains are used for at least reducing the levels of colonization by at least one target bacteria in animals, especially poultry.

Novel bacteriocins produced by novel bacterial strains are used for at least reducing the levels of colonization by at least one target bacteria in animals, especially poultry.

The invention discloses a dorking microorganism viable bacteria fermentation concentrated feed and a production method thereof. The dorking microorganism viable bacteria fermentation concentrated feed is prepared by mixing the following materials: 30-40% of biological decomposition bean pulp, 8-10% of polypeptide biological blood powder, 40-46% of mixed feed meal, 8-12% of feed additive and 1-5% of animal oil. In the feed, feed use ratio is improved by exogenous enzyme and microorganism fermentation; by the beneficial microorganism viable bacteria, flora in a dorking intestinal tract can be regulated to be balanced to improve the anti-stress capability of the dorking; and organic acid and bacteriocin generated by microorganism metabolism can inhibit and kill pathogenic bacteria in feed and the dorking body. The dorking microorganism viable bacteria fermentation concentrated feed does not need to add any antibiotic substance, can effectively inhibit undesirable bacteria growth, regulate the balance of egg and meat intestinal flora, and improve immunity; and chicken has abundant nutrition and does not contain any substance harmful to the human body.

Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.