The invention discloses bacillus velezensis ZF481 and application thereof. The strain number of the bacillus velezensis is ZF481, the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center on July 8, 2020, and the registration number of the bacillus velezensis is CGMCC No.20320. The bacillus velezensis ZF481 or / and the metabolite of the bacillus velezensis ZF481 can be used for preventing and / or treating the plant clubroot, or preparing a product for preventing and / or treating the plant clubroot. The strain has an inhibiting effect on the activity and germination of plasmodiophora brassicae resting spores, has a good growth promoting effect on brassicaceous crops such as Chinese cabbage and the like, and also has a good antibacterial effect on phytophthora capsici, stemphylium solani, fusarium oxysporum, alternaria solani, corynespora, Botryis cinerea, rhizoctonia solani and the like.