58 results about "Brassica cretica" patented technology

The invention discloses a method for detecting cadmium pollution through variation of genetic expression of a brassica plant. The method comprises steps as follows: extracting total RNA (Ribose Nucleic Acid) from a cadmium-processed brassicajuncea root and a compared brassicajuncea root; hybridizing with a 29K arabidopsis chip, so as to obtain a cadmium induced up-regulated gene and a cadmium induced down-regulated gene under specific expression; designing primers in conserved regions of genes; carrying out a semiquantitative PCR (Polymerase Chain Reaction) method to sieve and determine six up-regulated genes and six down-regulated genes with the specific expression of brassicajuncea when cadmium concentration is more than 1.00mg / kg based on a standard of increasing or reducing 2.00 timesof expression quantity; utilizing variation of gene expression in the root of the brassica plant in primer detection environments of 12 genes; if luminance of a product of the brassica plant in the environment to be detected and amplified by the primers of 6 up-regulated genes is increased over 2.00 times that of contrast, and the luminance of the product of the brassica plant in the environment to be detected and amplified by the primers of 6 down-regulated genes is decreased over 2.00 times that of the contrast, determining that concentration of cadmium in the environment to be detected is more than 1.00mg / kg, thus determining that whether the brassica plant in the environment to be detected is subjected to the cadmium pollution. The detection method is high in sensitivity and high in specificity, and can be used for quickly detecting whether content of the cadmium in the environment of the brassica plant is suitable for agricultural production.

The present invention relates to a tomato plant (Solanum lycopersicum L.) which may comprise a genetic determinant that confers resistance to Pepino Mosaic Virus (PepMV), wherein the resistance is characterised by the presence of at least QTL1 and / or QTL2. The invention also relates to sources for obtaining said genetic determinant, representative seed of which were deposited with the NCIMB under accession numbers NCIMB 41927, NCIMB 41928, NCIMB 42068, and NCIMB 42069. The invention further relates to seeds and progeny of the plant and to its fruits and processed fruits. In addition the invention relates to molecular markers linked to PepMV resistance conferring QTLs and the use thereof.

The invention discloses a dominant nuclear male sterility gene MS5 in Brassica napus b The invention and its application relate to the fields of rapeseed genetic breeding and genetic engineering. Brassica napus dominant nuclear male sterility gene MS5 provided by the invention b The functional segment protein, its amino acid sequence is shown in SEQ ID NO:1; its coding sequence is shown in SEQ ID NO:3. The gene can be used for transfection of dominant cell male sterile lines; the rapeseed dominant cell male sterile lines can be directly created by transgenic method. The male sterile line of rapeseed prepared by the present invention has no abnormality in the vegetative growth period, and produces male sterility only in the meiosis abnormality in the reproductive growth period, and can be applied to the use of heterosis and population improvement in Brassica crops. Breeding of Sedge crops is of great significance.

The invention discloses an application of LCYB (lycopene-beta-cyclase) and LCYE (lycopene-epsilon-cyclase) expression interference and EGL3 excessive expression in preparation of brassica plants with red petals, and further discloses an expression vector for interfering LCYB and LCYE gene expression and simultaneously excessively expressing EGL3 genes and a preparation method of the expression vector. Yellow carotenoid is inhibited and red lycopene is accumulated through interference for LCYB and LCYE gene expression, accumulation of anthocyanin is promoted through excessive expression of EGL3 genes, so that petals are red, the brassica plants with the red petals are prepared successfully, and the flower colors of the brassica plants are enriched.