57 results about "Brassica cretica" patented technology

The invention discloses a method for evaluating resistance of brassica plants on club root, which belongs to the brassica plant disease resistance evaluation technical field, and is characterized in that: perlite after being soaked by acid water is mixed with crushed brassica plasmodiophora brassicae fungus root according to a ratio of 1L: 10 to 15g to form culture matrix, the culture matrix is filled inside grids of a frozen box, seeds of a specimen to be evaluated and infected specimen seeds infecting the club root are planted inside the grids of the frozen box, one seed is planted inside each grid, the frozen box is arranged inside a ceramic dish, and 1/2MS culture matrix and the crushed brassica plasmodiophora brassicae fungus root are used for being made into inoculant liquid with pH value of 5.5 and content of pathogenic bacteria spores of 1*108/ml; and the inoculant liquid is filled into the ceramic dish to be cultured inside an artificial weather box, and the resistance of the specimen to be evaluated on the club root is evaluated according to the investigated disease indexes. Due to the adoption of the method, mass evaluation of the resistance on the club root can be realized, a great amount of land can be saved, consistence of the evaluation conditions can be guaranteed, time and labor can be saved, the evaluation result is accurate and reliable, and simplicity and convenience in operation can be realized.

The invention discloses a core primer composition for Brassica SSR (simple sequence repeats) and belongs to the technical field of molecular inheritance. The core primer composition comprises 190 core SSR primer pairs for 19 chromosomes in Brassica A and C genomes; and 10 SSR primer pairs are uniformly distributed on each chromosome, which have high polymorphism levels, substantially consistent annealing temperature and clear amplification band patterns. The core SSR primer composition is applied to increasing the molecular marking efficiency by 3 to 5 times without increasing the working costs, and is particularly suitable for the identification, the genetic diversity analysis, the nullisome and abnormal chromosome individual identification and the seed purity determination of the Brassica germplasm resources. The core SSR primer composition has the advantages of simpleness in operation, high stability and good reproducibility.

The invention discloses application of inhibiting accumulation of xantheins while accumulating lycopene and anthocyanin in preparation of Brassica plants with red pedals. The application is specifically as follows: interfering with the gene expression of LCYB, LCYE, MYB12 and MYB111 and simultaneously performing ectopic expression of TT8 genes. The invention further discloses a preparation expression vector for interfering with the gene expression of LCYB, LCYE, MYB12 and MYB111 and simultaneously performing ectopic expression of the TT8 genes and a preparation method of the vector. By interfering with the gene expression of LCYB, LCYE, MYB12 and MYB111, the xantheins, namely carotenoid and flavonol are inhibited; and by performing the ectopic expression of the TT8 genes, the accumulation of lycopene and anthocyanin can be promoted, so that the pedals are red, the Brassica plants with the red pedals are successfully prepared, and the flower colors of the Brassica plants are enriched.

The invention discloses cabbage-type rape as well as parent species Chinese cabbage and cabbage TT8 gene families and applications thereof, wherein, the Chinese cabbage TT8 gene family comprises a BrTT8-1 gene (SEQ ID No.1-2) and a Br TT8-2 gene (SEQ ID No.3-4); the cabbage TT8 gene family comprises a BoTT8-1 gene (SEQ ID No.5-6) and a BoTT8-2 gene (SEQ ID No.7-8); the cabbage-type rape TT8 gene family comprises a BnTT8-1 gene (SEQ ID No.9-10) and a BnTT8-2 gene (SEQ ID No.11-12). The above gene families can be used for molecule breeding of the brassica crop seed properties.

The invention discloses a novel selective breeding method for a male sterile line of Sichuan preserved pickle cytoplasm brasica napus. According to the method, a sterile source of the Sichuan preserved pickle is transferred into the brasica napus through distant hybridization and continuous backcrossing between different inter-species in brassica, so as to obtain Sichuan preserved pickle cytoplasm brasica napus CMS '93-12A'. The Sichuan preserved pickle cytoplasm brasica napus CMS '93-12A' is improved by hybridization and continuous backcrossing, so as to obtain a novel, stable, and double low sterile line material 'Jia rapeseed 58A'; the identification of the restoring and maintaining relationship of the Sichuan preserved pickle cytoplasm CMS shows that the restoring and maintaining relationship of the 'Jia rapeseed 58A' is different from the restoring and maintaining relationships of Pol CMS and Shaan 2A CMS, and meanwhile, one part of restorer material is screened out, and is improved to obtain five parts of double haploid restorer materials; the PCR identification with a specific primer shows that sterile sources of the '93-12A', the 'Jia rapeseed 58A' and mustard CMS are the same; an identification result of hybridization mating group yield of the 'Jia rapeseed 58A' and different restorer materials shows that a part of the combinations have a good yield-increasing potential, and the 'Jia rapeseed 58A' has a good combining ability. The method provided by the invention can be applied to the selective breeding of rapeseed varieties and the planting of the rapeseed in production.

The invention provides a culture medium formulation for regenerated plantlets of Brassica isolated microspores, which relates to improvement of a culture medium formulation for regenerated plantlets of Brassica microspores as horticultural crops, and belongs to the field of bioengineering. The invention provides the culture medium formulation for regenerated plantlets of Brassica isolated microspores, which is high in induced embryo formation rate and differentiation rate. A culture medium of the invention comprises an embryoid induction culture medium, a differentiation culture medium and a rooting culture medium, wherein the embryoid induction culture medium, the differentiation culture medium and the rooting culture medium all comprise macroelements, trace elements and organic elements. The formulation has the advantages of providing a large number of new genotype pure lines for breeding and greatly enriching breeding resources of Brassica.

The invention relates to the technical field of brassica and raphanus plant breeding, self-incompatible Chinese cabbages and self-incompatible radishes are taken as examples, when the Chinese cabbagesand the radishes enter an initial flowering stage and bloom in quantity, a potassium iodide solution of 1-10mM mixed with 0.025% Tween20 (for increasing the adhesiveness of the solution) is sprayed by a sprayer at 8: 00-10: 00 in sunny morning; the solution is sprayed on a cross-shaped stigma which is just unfolded until the surface of the stigma is stained with water drops. Spraying is performedfor the second time and the third time at an interval of 15 minutes. After spraying for the third time and the fog drops on the stigmas are completely dried, pollinating with fresh pollen, pinching off redundant unexpanded inflorescences, and sleeving with a seed collecting bag. The method is suitable for self-incompatible lines of brassica and raphanus plants. The potassium iodide solution is sprayed on stigmas, which are just developed into cross flowers, of inflorescences of self-incompatible Chinese cabbages (Brassica rapa L. Sp. Pekinensis) and self-incompatible radishes (Raphanus sativus) so that the self-affinity index can be increased to different degrees.

The invention discloses gene families of cabbage type rape, parental species Chinese cabbage and cabbage AHA10 and applications thereof, wherein the Chinese cabbage AHA10 gene family comprises a BrAHA10-1 gene (SEQ ID No.1-2) and a BrAHA10-2 gene (SEQ ID No.3-4); the cabbage AHA10 gene family comprises a BoAHA10-1 gene (SEQ ID No.5-6) and a BoAHA10-2 gene (SEQ ID No.7-8); the cabbage type rape AHA10 gene family comprises a BnAHA10-1 gene (SEQ ID No.9-10) and a BnAHA10-2 gene (SEQ ID No.11-12); and the gene families can be applied to the molecular breeding of the seed properties of brassica plants.

The invention discloses a method for rapidly screening farm chemical residues in vegetables. The method comprises the following steps: step 1, sample pretreatment; step 2, chlorophyll removal; step 3, qualitative analysis; step 4, quantitative analysis. In the first step, firstly, vegetable leaves of leaf vegetables are cut into pieces and put into a homogenizer to be ground uniformly, and part of pulp with skins of solanaceous vegetables, bulbs, brassica vegetables, melons and legume vegetables is put into the homogenizer to be ground uniformly, so that a sample is prepared. The method is safe and reliable, the screening method is high in screening speed and convenient to use, chlorophyll can be conveniently removed before screening by adding the step of removing chlorophyll in vegetables, sensitivity is improved, screening is convenient, and by using the screening method, 83 farm chemicals with high toxicity, common use and high residual risk and metabolite parameters thereof can be conveniently screened out, the types of the farm chemicals capable of being screened out are more comprehensive, and the method is convenient to popularize and use.

The invention discloses a method for simultaneously increasing the sulforaphane content and the selenium content in the edible organs of brassica vegetables. According to the method, in the squaring stage of Brassica oleracea or the bolting stage of Brassica alboglabra, 1-4 mM of a sulfur fertilizer is subjected to root application, and 40-150 [mu]M of a selenium fertilizer is sprayed to leaf surfaces. According to the invention, by using the method, the sulforaphane content in the edible organs of brassica vegetables can be effectively increased by 13.5-59%, and the content of organic selenium can be effectively increased by 11.9-99.5 times.

The invention discloses a Chinese cabbage TT8 gene family comprising two members, namely a BrTT8-1 gene with a full-length cDNA sequence shown as SEQ ID No.2 and a BrTT8-2 gene with a full-length cDNA sequence shown as SEQ ID No.4. The gene family can be applied to the molecular breeding based on seed traits such as seed coat color, seed size and the like of a brassica plant.

The invention mainly relates to the technical field of foods, and discloses milk-fragrance Chinese cabbage juice. The milk-fragrance Chinese cabbage juice is made from the following raw materials of Chinese cabbages, milk, soybean oligosaccharides, table salt, konjak starch, lactic acid bacteria, complex enzymes, a yeast extract and a purple perilla extract. The milk-fragrance Chinese cabbage juice is rich in milk fragrance, moderate in sour and sweet degrees, balanced in nutrients, extensive in raw materials and low in price; the deep processing ways of the Chinese cabbages are increased, and economic returns are increased by 10.3%; the Chinese cabbages are subjected to enzymolysis through complex enzymes, so that the juice yield rate is increased by 8.7%, the utilization rate of the Chinese cabbages is increased, and production cost is saved; the raw materials are subjected to fermentation twice, so that the milk-fragrance Chinese cabbage juice is sour and sweet in mouth feel and rich in nutrition, the growth of infectious microbes is restrained, and the shelf life of the milk-fragrance Chinese cabbage juice is prolonged; the konjak starch is added, so that the nutrients of the milk-fragrance Chinese cabbage juice are rich, the functions of the stomach and the intestines are promoted, the product state is stable, and the milk-fragrance Chinese cabbage juice is not layered; and various nutrient extraction agents are added, so that nutrients are increased, absorption is promoted, the infectious microbes can also be restrained, health-care functions are improved, additives are not contained, and the milk-fragrance Chinese cabbage juice can enhance immunity, beautify faces, protect skin, reduce weight, enable bodies to be slim, prevent dental caries and resist and restrain cancers.

The invention relates to a method for utilizing stamen petalody to mark male sterility genes of Brassica campestris ssp.chinensis, belongs to the field of a biotechnology and is used for marker-assisted selection breeding of a Brassica campestris ssp.chinensis male sterility line. The method comprises the following step of: amplifying DNA (Deoxyribonucleic Acid) synthesized by RNA (Ribonucleic Acid) inverse transcription of a stamen petalody line and a maintainer line thereof by utilizing a mark primer AFLP17 (Amplified Fragment Length Polymorphism 17) or a mark primer SCAR33, wherein if a 300bp segment can be amplified, the male sterility genes of the Brassica campestris ssp.chinensis do not exist. The method can be used for carrying out cDNA-AFLP and SCAR molecular marker sieving on the DNA synthesized by the RNA inverse transcription of the Brassica campestris ssp.chinensis stamen petalody line W053 and the maintainer line W052 thereof, can effectively expand the marker-assisted selection breeding of the Brassica campestris ssp.chinensis male sterility line, and greatly improves the selection efficiency, so that the breeding progress is accelerated.

The invention provides an application method and application of a reagent for eliminating the content of calcium ions in stigmas in selective fertilization of cruciferous plants, and relates to the technical field of cruciferous plant breeding. The application or the reagent is suitable for a self-incompatible line or a male sterile line with self-incompatibility of brassica crops. The method is easy and convenient to operate, easy to popularize, small in damage to plants, remarkable in effect of overcoming self-incompatibility, wide in application range and capable of being applied to brassica crops such as Chinese cabbages and cabbages and various radish crops; and in addition, the method can be further used for overcoming self-incompatibility of other cruciferous crops, and has important value for breeding and seed production of the cruciferous crops.