Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of inhibiting accumulation of xantheins and accumulating lycopene and anthocyanin in preparation of Brassica plants with red pedals

A kind of Brassica, petal technology, applied in the field of genetic engineering

Inactive Publication Date: 2015-04-01
SOUTHWEST UNIVERSITY
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the entire Brassica genus including rapeseed, there is no report on the use of molecular breeding for metabolic engineering of rapeseed flower color.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of inhibiting accumulation of xantheins and accumulating lycopene and anthocyanin in preparation of Brassica plants with red pedals
  • Application of inhibiting accumulation of xantheins and accumulating lycopene and anthocyanin in preparation of Brassica plants with red pedals
  • Application of inhibiting accumulation of xantheins and accumulating lycopene and anthocyanin in preparation of Brassica plants with red pedals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Microscopic observation of chromogenic subcellular organelles in Brassica and radish petals

[0042] Take fresh Brassica napus yellow petals (BnY); Brassica napus milk petals (BnW); Brassica napus yellow petals (BjY); Brassica napus yellow petals (BrY); White petals (BoW) and radish red petals (RsR) were stored on ice and shipped back to the laboratory, then sliced ​​by hand, observed with a low-power lens for quick screening, and qualified slices were selected for careful observation and photography. The results are as follows figure 1 shown. The results showed that the yellow substances in the yellow petals of several species of Brassica appeared as numerous small granules in one cell, distributed unevenly in the cell, and were squeezed to the wall-adhering position by the vacuoles, indicating that the yellow petals The chromogenic organelles are chromoplasts, whose pigments are yellow carotenoids. There are also some cells in the milky petals of Brassica...

Embodiment 2

[0043] Embodiment 2, detect brassica and radish petal pigment

[0044] In the morning of the full flowering period, the newly opened Brassica napus yellow petals (BnY); Brassica napus milk petals (BnW); Brassica napus yellow petals (BjY); BoY), kale white petals (BoW) and radish red petals (RsR), immediately transported back to the laboratory in a cool and fresh place, dried at 50-60°C, ground into powder, passed through a 60-mesh sieve, and stored in a dry place away from light.

[0045] 1. Test results of petroleum ether, hydrochloric acid and ammonia water

[0046] Weigh 0.100g of preserved petal powder, put them into numbered stoppered test tubes, add about 10mL of petroleum ether, 10% hydrochloric acid, and 30% ammonia water, mix gently, filter, and observe the color change. The results are as follows :

[0047] (1) Petroleum ether reaction: Brassica napus, Chinese cabbage rape, and mustard rape all show bright yellow, indicating that the content of carotenoids is high;...

Embodiment 3

[0067] Example 3. Molecular Identification of Brassica and Radish Petal Pigment Biosynthetic Pathways

[0068] (1) Expression characteristics of flavonoid pathway genes in Brassica and radish petals

[0069] In order to study the molecular mechanism of the difference in petal pigments between Brassica and radish, RT-PCR detection primers for the flavonoid pathway genes of Brassica and radish were designed, and 5SrRNA was used as an internal reference, as shown in Table 1:

[0070] Table 1. Primers for RT-PCR detection of flavonoid pathway

[0071]

[0072]

[0073]In the morning of the full flowering period, the newly opened Brassica napus yellow petals (BnY); Brassica napus milk petals (BnW); Brassica napus yellow petals (BjY); BoY), kale with white petals (BoW) and red radish petals (RsR), transported frozen in liquid nitrogen, and stored in a -80°C refrigerator for later use. Then use the RNAprep Pure Plant Total RNA Extraction Kit to extract the total RNA, after el...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of inhibiting accumulation of xantheins while accumulating lycopene and anthocyanin in preparation of Brassica plants with red pedals. The application is specifically as follows: interfering with the gene expression of LCYB, LCYE, MYB12 and MYB111 and simultaneously performing ectopic expression of TT8 genes. The invention further discloses a preparation expression vector for interfering with the gene expression of LCYB, LCYE, MYB12 and MYB111 and simultaneously performing ectopic expression of the TT8 genes and a preparation method of the vector. By interfering with the gene expression of LCYB, LCYE, MYB12 and MYB111, the xantheins, namely carotenoid and flavonol are inhibited; and by performing the ectopic expression of the TT8 genes, the accumulation of lycopene and anthocyanin can be promoted, so that the pedals are red, the Brassica plants with the red pedals are successfully prepared, and the flower colors of the Brassica plants are enriched.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to the application of inhibiting yellow pigment accumulation and simultaneously accumulating lycopene and anthocyanin in preparing Brassica plants with red petals. Background technique [0002] Rapeseed is an important oil crop with a long history of cultivation, high economic value, wide application and strong adaptability. It is the largest oil crop in my country and an important oil crop worldwide. In terms of species taxonomy, rape is composed of three species of Brassica, Brassica napus (2n=38, AACC), Brassica rapa ssp.oleifera syn.B. campestris, 2n=20 , AA), Brassica juncea (2n=36, AABB). Brassica napus is a compound species evolved from natural interspecific hybridization of Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea, 2n=18, CC), although the history of cultivation is only a few hundred years , but because of its strong growth potential and h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/61C12N15/82C12N15/66C12N1/21A01H5/00C12R1/01
Inventor 柴友荣刘雪董博廖霏霏林呐殷家明周清源唐章林王倩
Owner SOUTHWEST UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com