91 results about "Ectopic expression" patented technology

Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, or low nutrient conditions.

Polynucleotides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The encoded polypeptides of the invention have been shown to confer at least one regulatory activity and confer greater size, greater organ size, greater biomass, greater yield, curlier leaves, darker coloration, greater tolerance to water deprivation, delayed flowering, delayed development, delayed senescence, greater tolerance to cold, and / or greater tolerance to hyperosmotic stress as compared to a control plant.

Disease-inducible promoter sequences have been identified that may be used to produce transgenic plants that are both more resistant to disease than control plants, and are wild-type or nearly wild type in appearance. Any of these disease-inducible promoters may be incorporated into expression vectors that each comprise a defense response protein operably linked to the promoter. The expression vectors can be introduced into plants and the defense response protein then ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, in some cases, to more than one type of pathogen, and yet are similar to wild type plants in their morphology and development.

The invention provides methods for generating induced pluripotent stem (iPS) cells from normal and mutant adult cells, as well as the iPS cells so generated from such methods. In some aspects, iPS cells are generated by ectopically expressing SOX2 and OCT4 nucleic acids in such adult cells. Other nucleic acids such as but not limited to MYC may also be ectopically expressed in such adult cells in the methods described herein.

Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, or low nutrient conditions.

The invention discloses a rice cadmium-tolerant gene OsGSTU37 and application thereof.Sequences of the rice cadmium-tolerant gene OsGSTU37 are shown as SEQ ID NO:1 in sequence tables, and amino acid sequences of glutathione S-transferase (GSTs) of an encoding protein of the rice cadmium-tolerant gene OsGSTU37 are shown as SEQ ID NO:2 in the sequence tables.As proved by ectopic expression of yeast, the rice cadmium-tolerant gene OsGSTU37 has cadmium toxicity tolerant characteristics.The rice cadmium-tolerant gene OsGSTU37 and the application have the advantages that knockout lines and over-expression lines of the rice cadmium-tolerant gene OsGSTU37 are constructed, as discovered in seed germination comparative experiments on the knockout lines, the over-expression lines and wild type rice, the knockout lines of the rice cadmium-tolerant gene OsGSTU37 are poor under cadmium stress, but the over-expression lines of the rice cadmium-tolerant gene OsGSTU37 and the wild type rice can germinate, and germination and growth of the over-expression lines of the rice cadmium-tolerant gene OsGSTU37are superior to germination and growth of the wild type rice; important effects of the rice cadmium-tolerant gene OsGSTU37 in cadmium-tolerant mechanisms can be equivalently described; over-expression vectors of the rice cadmium-tolerant gene OsGSTU37 are constructed, and plants are transformed by the aid of the over-expression vectors, so that cadmium-tolerant plant varieties can be obtained, the cadmium stress resistance of the plants can be improved, and theoretical bases can be provided for breeding novel cadmium-tolerant crop varieties.

The present invention provides polynucleotides encoding CCAAT-binding transcription factor polypeptides that modulate the onset of reproductive development in plants. Polynucleotides encoding functional CCAAT-binding transcription factors were incorporated into expression vectors, introduced into plants, and ectopically expressed. The encoded polypeptides of the invention significantly shortened the time to flower development in the transgenic plants, as compared to the flowering time of control plants.

The invention relates to a method for combating disorders affecting the mitochondrial oxidative phosphorylation system by allotopic expression of the cyanide-insensitive alternative oxidase (AOX) in human cells. The successful expression of AOX in human cells and in Drosophila has been shown to confer spectacular cyanide-resistance to mitochondrial substrate oxidation, alleviate oxidative stress, apoptosis susceptibility and metabolic acidosis. AOX is well tolerated when expressed ubiquitously in the whole organism. AOX expression is a valuable tool to limit the deleterious consequences of respiratory chain deficiency.

Methods for the identification of agents the bind to exo-sites of proteins are provided. Agents identified by the methods described herein and pharmaceutical compositions comprising the identified agents are also provided. Methods of using an identified agent for the treatment or prevention of a disease, disorder, or condition are also provided, including methods of treating or preventing a disease associated with reduced, elevated, or ectopic expression or aberrant activity of a protein comprising an exo-site.

The present invention provides a non-naturally occurring seed plant that is characterized by delayed seed dispersal due to ectopic expression of a nucleic acid molecule encoding an AGL8-like gene product. Further provided herein is a non-naturally occurring seed plant, such as an agl1 agl5 double mutant, that is characterized by delayed seed dispersal due to suppression of AGL1 and AGL5 expression in the seed plant. The invention also provides a substantially purified dehiscence zone-selective regulatory element, which includes a nucleotide sequence that confers selective expression upon an operatively linked nucleic acid molecule in the valve margin or dehiscence zone of a seed plant. Also provided by the invention are kits for producing a transgenic seed plant characterized by delayed seed dispersal, such kits containing a dehiscence zone-selective regulatory element.

Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, or low nutrient conditions.

To produce early flowering genotypes, plum (Prunus domestica) was transformed with the poplar (Populus trichocarpa) Flowering Locus T1 (PtFT1) gene. Ectopic expression of 35S::PtFT1 Induced early flowering in vitro from transgenic plantlets within two months of transformation. When the transgenic plum plants were rooted and transferred to soil and grown in posts in the growth chamber, a number of additional lines flowered. Normal flowering and fruiting were observed in the greenhouse within one year of transformation. While dormancy was not necessary for growth or fruiting, FT plums were still winter hardy and floral bud set and flowering responded normally to changes in temperature. By manipulating a single gene, temperate tree crops can be effectively engineered for cultivation in new growing areas and for entirely new modes of agricultural production that are continuous, sustainable, and adaptable to climate change.

The present invention provides nucleic acid constructs, including plasmids, expression vectors or expression cassettes comprising polynucleotides encoding CCAAT-binding transcription factor polypeptides that have the ability to increase a plant's tolerance to abiotic stress. Polynucleotides encoding functional CCAAT-binding transcription factors were incorporated into expression vectors, introduced into plants, and ectopically expressed. The encoded polypeptides of the invention significantly increased the cold and water deficit tolerance of the transgenic plants, as compared to tolerance to these stresses of control plants.

The present invention provides methods of selectively controlling lignin biosynthesis in plants such that lignification is reduced or enhanced, as desired. The invention provides, for example, a method of reducing lignification in a vascular plant by ectopically expressing a nucleic acid molecule encoding an AGL8-like gene product in the plant, whereby lignification is reduced due to ectopic expression of the nucleic acid molecule. An AGL8-like gene product useful in the invention can have, for example, substantially the amino acid sequence of an AGL8 ortholog such as Arabidopsis AGL8 (SEQ ID NO:2).