The invention discloses a construction method of a
lung cancer radiation resistance
cell strain. The method includes the following steps of inoculating
lung cancer cells in a six-hole plate when the
lung cancer cells are conventionally bred to the logarithmic phase, placing the six-hole plate at the center of an
irradiation field, covering the bottom face of the six-hole plate with
tissue equivalent filler 1.5 cm thick, perpendicularly transmitting 6MV-X rays according to the
dose rate of 300 cGy / min and the source
skin distance of 100 cm, sequentially conducting
irradiation according to the
dose of 2 Gy, 4 Gy, 6 Gy, 8 Gy and 10 Gy each for two times till the
total dose reaches 60 Gy, continuing to conduct breeding and passage to five or more generations till the
cellular morphology and multiplication are stable, and conducting
cryopreservation for standby application. The construction method is small in
irradiation time number, short in construction time, small in
cell contamination probability and superior to a small-
dose equal-segmentation irradiation method; the
colony formation experimental result shows that the
radiation resistance of the
lung cancer radiation resistance
cell strain constructed according to the method is higher than that of
lung cancer radiation resistance cell strains constructed according to the sublethal dose irradiation method.